Basic Informations

C.V

Teaching Experience:     

 

Under-Graduate students:

2007-2011: Pharmacognosy department, school of pharmacy, BeniSuef University:

 Medicinal plants, Pharmacognosy 1, 2 and 3, phytochmesirty I and II, phytomedicine and   herbal medicine, quality control of natural products

2008-2011: Pharmacognosy department, school of pharmacy, NUB University:

Medicinal plants, Pharmacognosy 1, 2 and 3, phytochmesirty I and II

2011-present: Phytochemistry I and II, quality control of natural products

Graduate students:

2007-present: chromatographic techniques advanced chromatographic techniques, 1D and 2D spectral elucidation of Natural products.

MAIN RESEARCH:

Natural Product Chemistry, with special concentration on the chemistry of macro and micro marine organisms.

 

Publications:

1997:

Master in Pharmaceutical Science: Thesis Title (Phytochemical Screening of Aberia caffra Planted in Egypt)

2006

PhD in Pharmacognosy: Dissertation Title (Bioactive Metabolites from Stylissa and Plakortis Marine Sponges)

Master Title

Phytochemical study of Aberia caffra plant

Master Abstract

Alkaloids from the Leaves of Aberia caffra Article in Indian Journal of Chemistry Section B 39(3):215-219 · March 2000 Abstract Two new alkaloids have been isolated from the leaves of Aberia caffra, and characterised as aberiamine N1 pentyl-10-(1,3-dimethylhexahydro-2-pyrimidinyl)-1-dacanamine and aberiamide N-1[{E)-1-butenyl 17-dimethylcarboxamidoheptadecyl)amino}methyl]-N-1-methylacetamide. In addition to a- ,ß-amyrin, ß-sitosterol and its glucoside, three flavonoids, have also been isolated. Identification of these compounds has been done by spectral analysis and comparison with authentic samples.

PHD Title

Phytochemical study of some marine sponges

PHD Abstract

ABSTRACT Marine invertebrates and in particular sponges continue to be a plentiful source of bioactive secondary metabolites. One especially promising class of compounds continues to be the modified peptides. Structurally this class includes linear peptides, peptide lactones, cyclic peptides and depsipeptides. Cyclic peptides are common metabolites from sponges, and a number of these peptides have been isolated and identified from different species of the genus Phakellia and Axinella sponges, and were reported to have cytotoxic activity against a number of cancer cell lines. An investigation of active sponge extracts yielded two new cyclic heptapeptides, stylisin 1 and 2 and phakellistatin 13 in addition to the alkaloids, oroidin, stevensine, and sceptrin from the sponge Stylissa caribica. Phakellistatin 13 was previously isolated from the sponge Phakellia fusa Thiele, and reported to have potent cytotoxic activity against the human hepatoma BEL-7404 cell line with an ED50 < 0.01 µg/mL. However, phakellistatin 13 was isolated from Stylissa caribica sponge, was elucidated independently, and was proven to be non-cytotoxic in the NCI tumor cell panel and against Vero cells. The new structures were assigned based on the 1D and 2D NMR spectroscopic data, as well as chemical methods for the elucidation of the absolute configuration of the amino acids. The new peptides, phakellistatin 13 and the alkaloids have been evaluated for their antimicrobial, antiprotozoal, antiHIV-1, antiMtb, antitumor and anti-inflammatory activities, and were not active. The sponges of the genus Plakortis from the marine environment have been known to produce a variety of bioactive natural products, especially cyclic peroxide containing vii metabolites. These metabolites have been found to exhibit a diverse range of bioactivities including anticancer, antibacterial, antifungal and Ca2+ uptake by the cardiac sarcoplasmic reticulum. The new cyclic peroxide, plakortisinic acid, a new ketone derivative, an a,ß- unsaturated ester, plakortide F and its free acid, plakortone D, plakortide N, and a furan containing metabolite, were isolated from a Jamaican Plakortis sponge. The structures were elucidated by interpretation of the 1D, 2D NMR, MS data and by comparison with data from the literature values. The free acid of plakortide F was shown to have activity against a number of human diseases such as Leishmania donovani with an IC50 of 2.8 µg/mL, C. albicans with an IC50 of 1.5 µg/mL, and was reported to have cytotoxic activity against P388 murine leukemia with an IC50 of 0.5 µg/mL. Chemical transformations of this metabolite have been accomplished in order to optimize the activity and evaluate the SAR. Thirty-five semisynthetic analogs were prepared, purified and the structure of each was confirmed with 1H, 13C-NMR and HRMS spectroscopy. The natural products as well as the synthetic analogs have been assayed against several human diseases. This is the first report of the a,ß-unsaturated ester and plakortone D potently inhibited HIF-1 factor with 95% inhibition at a concentration of 5ppm. Several analogs were more active than the parent compound as antimalarial, antiMtb and antiHIV-1 agents. The volatile oil 2-decanone was successfully isolated in high yield from another common Caribbean Plakortis sponge. It has been determined that 2-decanone is the metabolite responsible for the fragrant odor and control of biofouling for this sponge. Results from our biofouling studies using zebra mussels (Dreissena polymorpha) have shown that 2- viii decanone has significant antifouling properties when coated on the surface of an easily fouled substrate.