Evaluation of different media for Isolation of dermatopytes

Dermatophytosis ( tinea or ringworm ) of the scalp , glabrous skin, and nails is caused by a closely related group of fungi known as dematophytes which have the ability to utilize keratin as a nutrient source , I . e they have a unique enzymatic capacity ( keratinase) . The disease process in dermatophytosis is unique for two reasons: Firstly , no living tissue is invaded the keratinized stratum corneum is simply colonised . however , the presence of the funguus and its metabolic products usually induces an allergic and inflammatory eczematous response in the host . the type and serverity of the host response is often related to the species and strain of dermatopyte causing the infection . Secondly , the dermatopytes are only fungi that have evolved a dependency on human or animal infection for the survival and dissemination of their species . The inicidence of dermatopytosis is on the increase every day. The disease is more common in poor countries and lower standards of hygiene . The aim of this work was to identify dermatopytes in different clinical forms using three types of selective media to evaluate the efficacy of eache on of them . 1- Sabouraud , s dextrose agar supplemented with cycloheximide and chloramphenicol. 2- Sabouraud – gentamicin – chloramphenicol containing ( SGC) 50 mg-ml gentamicin . 3- Sabouraud gentamicin – chloramphenicol containing ( SGC) 100 mg-ml gentamicin. This study involved fifty cases suffering from different forms of dermatophytosis . they selected from the el.enini hospital during the period from November 2008 to may 2009 . were subjected to clinical examination . The specimens were collected in petri dishes or piece of paper . Then direct microscopic was done using koh 10-20 % or lactophenol