Botanical features and genetic profiling of Bauhinia retusa Roxb. growing in Egypt

Research Abstract

Bauhinia retusa Roxb. (Caesalpinioideae) is a large semi-deciduous tree cultivated in Egypt. In this work, for the first time, botanical features including micro- and macro-morphological studies of different organs of B.retusa Roxb. As well as, DNA fingerprinting were brought to light. This study presents an examination of the botanical features of the leaves, petioles, stems and flower buds through microscopical observation of the prepared entire, transverse sections and isolated elements of these organs using light microscope. Also, Extraction of the Deoxyribonucleic acid (DNA) of B. retusa Roxb. from leaf samples and Random Amplification of Polymorphic DNA (RAPD) analysis was done using ten primers of arbitrary sequences. Resulting in 22 fragments of amplified product, which were generated by ten primers; the primer Operon C-19 produced the highest number of rapid amplified polymorphic (RAPD) fragments (4 fragments), while the least number of fragments was produced by Operon A-18, Operon B-2 and Operon B-9 (1 fragment). Micro- and macro-morphological characters in contribution to DNA fingerprinting can be considered as the identifying parameters to authenticate and recognize B. retusa Roxb.at the anatomical and genetic level.

Research Keywords

Botany; DNA fingerprinting; Fabaceae; Plant anatomy; RAPD