spectrophotometeric methods for analysis of different dosage form contaning pyridoxine hydrochloride

Research Abstract

In this section, modified area under the curve (MAUC) and mean centering of ratio spectra spectrophotometric (MCR) methods were used for selective determination of PYH, CYH and MEH. In the first method, PYH was determined by measuring the amplitude value of the plateau at 283 nm obtained after division on standard spectrum of 20 µg mL-1 of PYH. Where the areas under the curve in the ranges of 215-228 nm for CYH and 230-243 nm for MEH in the subtracted ratio spectra were used for CYH and MEH determination. In the developed mean centered of ratio spectra spectrophotometric method (MCR), the amplitudes of the second mean centered ratio spectra at 228.8, 260.2 and 270.8 nm for PYH, CYH and MEH, respectively were measured. Selectivity of the methods was tested by application to different synthetic mixture where good results were obtained. On the other hand, accuracy was verified by application of standard addition technique on Emetrex® and Dizerest B6® tablets where no interference from additives was found.

Research Keywords

cyclizine hydrochloride