Fedawy HS, Salama SS, Ali A, Rabie NS, Elkady MF. Phenotypic and genotypic Characterization of Avibacterium paragallinarum isolated from layer chicken Flocks in Egypt yearling 2013-2015. American Journal of Research Communication 2016, 4(12): 23-34

Research Abstract

Aim: This study was carried out for isolation of Av. paragallinarum from suspected clinical samples and to be identified and serotyped by the molecular technique using Multiplex PCR as an alternative serotyping method. Material and methods: One hundred and twenty field samples were collected from different poultry farms and bacteriologically examined and morphologically characterized. The suspected isolates which have a phenotypic characters as Av.Paragallinarum were examined to the serotype serovare level using PCR through two steps. The first used universal primer to check for Av. Paragallinarum with specific PCR primers yielding a 500 bp PCR product. The positive reactors with the primary PCR were proceeded to the second step which is multiplex PCR using primer sets specific for serotypes A, B, and C and the obtained PCR products were 0.8, 1.1 and 1.6 kbp fragments for serovars A, B and C, respectively. Result: Nine Av. paragallinarum isolates were identified, 4 isolates were serovar A, 3 isolates were serovar C and 2 isolates were serovar B. Conclusion: This study concluded to, the co-circulation of the Av. paragallinarum serotypes A, B and C in Egypt. Also the reliability of using multiplex PCR (hypervariable region of HMTp210) as a useful tool for fast serotyping of Av. Paragallinarum isolates.

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