Biochemical and Histopathological Studies on Antidiabetic and Radioprotective Effects of Green Tea in Gamma-Irradiated Albino Rats with Diabetes

Research Abstract

Diabetes Mellitus is a serious, chronic disease that represents one of the leading causes of mortality and morbidity worldwide. This work aimed to evaluate the role of green tea extract (GTE) at dose 200 mg/kg b.w to ameliorate the deleterious effects of ALX only and with gamma radiation on biochemical changes. Methods: Histopathological studies for liver, kidney and pancreas at the end of 21 days from administration with GTE were conducted. Injection of Alloxan (ALX) with dose 150 mg/kg B.W in male albino rats weighted 180-220 g that exposed to gamma radiation with fractionated separately dose of 3 and 5 Gy. Results: The results revealed the ability of GTE to reduce significantly the increment in blood glucose level in diabetics group and diabetics irradiated group with 3 and 5Gy by 53.3%, 42.3%, and 46.4% respectively. Albumin and liver enzymes ALT and AST levels were improved when diabetics group and diabetics irradiated groups were treated with GTE. Treatment with GTE showed improvement in urea level by 60.6%, 39.2%, and 45.3%, and in creatinine level by 54.8%, 38.5%, and 43.6% in case of the diabetic group and diabetic irradiated group with 3 and 5Gy. The histopathological findings were confirmed with that obtained by biochemical results for all experimental groups as compared to diabetic and diabetic irradiated groups. Conclusion: GTE has high potential to be used as hypoglycemic and radiaprotector agent after being administrated for 3 weeks in diabetic and diabetic irradiated rats.

Research Keywords

Diabetes, Green Tea Extract, LC/MS, Gamma radiation, Biochemical, Histopathological observations