Basic Informations
C.V
Sex Male | Date of birth 15/03/1985 | Nationality Egyptian
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POSITION
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Assistant Lecturer at Department of Microbiology and Immunology
Faculty of Pharmacy, Beni-Suef University (Egypt).
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September 2014 – June 2016 Vice Director of Innovation, Patency and Research excellence Support
Office
Beni-Suef University (Egypt).
January 2014 – Present Assistant Lecturer at Department of Microbiology and Immunology
Faculty of Pharmacy, Beni-Suef University (Egypt).
January 2011 – July 2011 Vice Director of Computer Center
Faculty of Pharmacy, Beni-Suef University (Egypt).
March 2009 – January 2014 Demonstrator at Department of Microbiology and Immunology
Faculty of Pharmacy, Beni-Suef University (Egypt).
December 2007 – November 2008 Military Pharmacist
Highstep Military Hospital, Armed Forces (Egypt)
Cairo (Egypt).
April 2014 – Present PhD study (Biotechnology)
Thesis Title: Investigation for the production of biologically active
secondary metabolites recovered from some microbial samples.
Faculty of Pharmacy, Beni-Suef University (Egypt)
Techniques included:
Isolation of microorganisms, Detection of Antimicrobial Activity,
Fermintation, Optimization of Natural Products Production and Extraction,
Natural Products Purification and Characterization, HPLC, LC mass,
16S rRNA, Bioinformatics, PCR and Gel Electrophoresis.
July 2010 – December 2013 M.Sc. (Microbiology)
Thesis Title: 'Distribution of genes encoding biofilm formation amongst
clinical isolates of Pseudomonas aeruginosa'.
Faculty of Pharmacy, Beni-Suef University (Egypt)
Techniques included:
Isolation of microorganisms, Antimicrobial Susceptibility Testing,
Biofilm Detection, Bioinformatics, PCR and Gel Electrophoresis.
May 2009 – March 2010 Premaster study (Microbiology & Immunology)
Faculty of Pharmacy, Beni-Suef University (Egypt)
Courses studied:
(Microbial sterilization and Biological and Quality Control), (Immunological
techniques and products and its Applications), (Genetics of Micro-
organisms and Genetic Engineering) and (Antimicrobial Sterilization and
Microbial Resistance), (Computer and its Application), (Literature Search),
(Scientific English Language) and (Basic statistics).
September 2002 – May 2007 Bachelor of Pharmaceutical Sciences
Faculty of Pharmacy, Beni-Suef University (Egypt)
Subjects studied:
Microbiology and Immunology, Pharmaceutics, Pharmaceutical Chemistry,
Organic Chemistry, Analytical Chemistry, Biotechnology, Biochemistry,
Pharmacognosy, Toxicology and Pharmacology.
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Publications
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'Detection of biofilm genes among clinical Isolates of Pseudomonas aeruginosa recovered from some Egyptian Hospitals.' N. Egypt. J. Microbiol., 36, (2013).
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Seminars
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-"Culturing the unculturable bacteria, January 2016"
Faculty of Pharmacy, Beni-Suef University (Egypt).
-"Investigation for the production of biologically active secondary metabolites from microbial samples, February 2014"
Faculty of Pharmacy, Beni-Suef University (Egypt).
-" Use of PCR and Gel electrophoresis, January 2012"
Faculty of Pharmacy, Beni-Suef University (Egypt).
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Conferences
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-Biotechnology for the emerging economies Conference at Beni-Suef University, Egypt, April 2015
Beni-Suef University (Egypt).
-Egypt invents Conference at Beni-Suef University, Egypt, March 2015"
Beni-Suef University (Egypt).
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Courses and Workshops
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-Workshop on how to submit a patent, November 2014
Academy of Scientific Research (Egypt).
-In-country Training Workshop on Science, Technology and Innovation Indicators, September 2014
Guesthouse at Ain-Shams University in Cairo with NEPAD/ASTII resource persons (Egypt).
-Training course of Faculty and Learning Development including different lectures and workshops on (how to publish international paper, how to organize conference and how to write a project proposal) , December 2013
Faculty and Learning Development Center (FLDC) at Beni-Suef University (Egypt).
-Workshop on how to use Endnote to write bibliography, July 2012
Faculty of Pharmacy, Beni-Suef University (Egypt).
- Molecular Biology Course, July 2011
Medical Technology Center at Alexandria University (Egypt)
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Funding
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Annual call for projects of Beni-Suef University
High throughput investigation for novel antimicrobial compounds
recovered from different wild types microbiota designed for pharmaceutical applications, 50,000 L.E, November 2015.
Beni-Suef University (Egypt).
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Mother tongue(s)
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Arabic
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Other language(s)
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UNDERSTANDING
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SPEAKING
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WRITING
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Listening
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Reading
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Spoken interaction
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Spoken production
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English
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B2
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B2
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B2
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B2
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B2
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IELTS Academic Exam (Band 6.5)
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Communication skills
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- Team work: I have worked in various types of teams from research teams to volunteering work.
- Presentation skills: I had presented many contents and materials during my work in both Faculty of Pharmacy and Beni-Suef University.
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Organizational skills
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- Organizer person in the organization committee of “Towards registration of 100 patent from Beni-Suef University Info Day, Egypt, September 2015”.
- Organizer person in the organization committee of " Biotechnology for the emerging economies Conference at Beni-Suef University, Egypt, April 2015".
-Organizer person in the organization committee of "Egypt invent Conference at Beni-Suef University, Egypt, March 2015".
- Organizer person in the organization committee of “How to register patent from Beni-Suef University Info Day, Egypt, September 2014”.
-Organizer person in the Scientific group at Beni-Suef Pharmacists Syndicate (July 2012 - February 2013)
Activities: Participating in the organization team of Professional pharmacist course, Pharmacovigillance course and Virus C course.
-Organizer person in the Social committee at Beni-Suef Pharmacists Syndicate ( August 2011 - July 2012 )
Activities: Participating in the organization team of Pharmacists day Conference and Social Ramadan Breakfasting day for the pharmacists.
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Computer skills
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-Very good in Microsoft Office programs (Word, Excel and PowerPoint) and Photoshop.
-Good in BioEdit, CLC Sequence analyzer and Endnote.
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Master Title
Distribution of genes encoding biofilm formation amongst clinical isolates of Pseudomonas aeruginosa'.
Master Abstract
Biofilm formation in Pseudomonas aeruginosa (P. aeruginosa) is controlled by about 1% of its chromosomal genes; from which four genes were selected for prospective work. The aim of this study was to determine the biofilm formation in P. aeruginosa clinical isolates and to evaluate the role of the selected genes in biofilm formation. A total of fifty isolates were recovered from different clinical samples isolated from Egypt by isolation on cetrimide agar media. These isolates were then identified by gram stain, culture characters and biochemical reactions. All the fifty isolates were identified as P. aeruginosa. The antimicrobial resistance pattern of the planktonic cells of all isolates was then determined. The antibiogram of isolates showed that amikacin was the most potent antibiotic against all isolates followed by ceftazidime and imipenem. Biofilm quantification (matrix, live and dead cells) of isolates was done by the microtiter plate method using crystal violet (CV) assay. According to the optical density (OD) readings, isolates were classified into the following categories: strong, moderate or weak biofilm producers. Result of CV assay showed that 42 isolates (84%) were strong biofilm producers; 4 isolates (8%) were moderate biofilm producers and the last 4 isolates (8%) were weak biofilm producers. Quantification of the metabolic activity of cells within biofilms (live cells only) was done by the microtiter plate method using XTT reduction assay. Then data analysis and comparison between results of both CV assay and XTT reduction assay was done. Data analysis revealed that there was a significant difference between the results of both assays. Finally, studying the effect of four selected genes (RhlI, PilA, PilT, PelB) on biofilm formation in 13 isolates (9 strong biofilm producer isolates and 4 weak biofilm producer isolates) was done using PCR technique. Screening of these genes revealed their presence in all screened isolates including both strong and weak biofilm producer isolates. These final results suggested the importance of these genes in biofilm formation and suggested the presence of other factors which may contribute in determining the degree of biofilm formation in P. aeruginosa.
PHD Title
Investigation for the production of biologically active secondary metabolites recovered from some microbial samples
PHD Abstract