Basic Informations
C.V
Curriculum
Vitae
Khalid Shaaban Hashem Mohamed
Lecturer
f biochemistry, chemistry of nutrition and molecular biology
Faculty of
veterinary medicine, Beni Suef University, Egypt.
Emails: Khalid_hashem@yahoo.com ,
kshashem@gmail.com
, khashem@vt.edu
Cell phone : 002/ 01226421228.
Personal information:
Full name: Khalid Shaaban Hashem Mohamed.
Date of birth: August, 4 1981.
Current
position: Lecturer of
biochemistry, chemistry of nutrition and molecular biology
Address: El Zahraa st, Beni Suef.
Education:
2008- 2011: Ph.D. Degree in vterinary
Science (Biochemistry, chemistry of nutrition and molecular biology) through a
channel system between Beni-Sueif University, Egypt and Prof. Dr. M. Renee
Prater, Ass. Professor of Pathology and immmuonolgy, School of osteopathic
meducine, University of Virginia Tech. Blacksburg, Virginia, USA. Thesis title,
“Biochemical effects of some antioxidants on induced liver toxicity”.
2005- 2007: Masters Degree in veterinary Sciences (Biochemistry, chemistry of nutrition and molecular
biology) Beni Suef University, Egypt. Thesis title, “Biochemical studies on
Catfish in Beni Suef governorate”
2004- 2005: Pre-requisite graduate courses towards the M.Sc.,
degree: (with general grade very good),
Faculty of vet. medicine, Beni Suef university, Academic year.
Pre-master Courses Include: (Biochemistry,
Physiology, Endocrinology, and Toxicology, Exophthalomolgy).
1998- 2003: Bachelor of veterinary science, Vet. College, Cairo University
(Beni Suef Branch), Egypt Grade (Very Good).
Career history:
23-8-2011 up till Now: Lecturer of Biochemistry and chemistry of
nutrition, Department of Biochemistry,
chemistry of nutrition, Faculty of veterinary medicine, Beni-Sueif University,
Egypt.
2011 up till now: Lecturer at Department of Laboratory assistants and
Technians, Medical Technicians Institute, Ministry of Health and Population.
Beni Suef Branch.
2012- 2014: Teaching
General biochemistry, Metabolism and clinical biochemistry at faculty of
pharmacy, Beni Suef University for regular students and clinical
program students.
23-5-2007 to 23-8-2011: Assistant lecturer of Biochemistry, Department of Biochemistry, chemistry of nutrition and molecular
biology, Faculty of veterinary medicine, Beni-Sueif University, Egypt.
11-1-2004 to 23-5-2007: Demonstrator
(equivalent to U.S graduate research assistant), Department of Biochemistry,
chemistry of nutrition and molecular biology, Faculty of veterinary medicine,
Beni-Sueif University,
Egypt.
Teaching experience:
? Teaching Practical biochemistry to 2nd year
undergraduate veterinary students.
? Responsibilities include preparing and giving some lectures of Chemistry
of nutrition and molecular biology.
? Acted as a liaison between 2nd year undergraduate
veterinary students and professors.
? Teaching Practical biochemistry to 2nd year
undergraduate veterinary students.
? Responsible for the practical exams of Pharmaceutical
Chemistry course.
?Teaching Practical courses to Postgraduate veterinary students
as (Clinical biochemistry, biochemistry of Hormons, Pharmacological
biochemistry,…….etc.)
? Teaching Medical biochemistry courses to 2nd
year undergraduate veterinary students.
? Responsible for the supervision on practical Biochemistry
course and exams.
? Teaching courses of Biochemistry to postgraduate students in our
faculty.
?Teaching
Courses of Hematology and Hematological disorders to 2nd year
students.
? Teaching
courses of clinical Biochemistry to 1st – 2nd year
students.
Technical experiences:
Some of the Techniques and Methods that I am familiar with
I- All techniques of the routine medical
analyses.
II- Highly expert in establishing new
medical laboratories and preparing all the stock chemical reagents and stains
that are used in them.
III- Research techniques:
PCR
Spectrophotometeric analysis of enzyme activities,
Immunocytochemistry
ELISA
AWARDS:
Scholarship from the Egyptian Government to perform the practical
part of my Ph.D under the supervision of
Dr. R. M. Prater Ass. Prof. of Pathology and Immunology, Faculty of
Osteopathic medicine, Virginia Tech. University, Blacksburg, Virginia, US.
Projects:
Funded project from Beni Suef university on (the possible
protective effect of the use of stem cell on nduced atherosclerosis)
Publications:
1- The
protective effects of cerium oxide nanoparticles against hepatic oxidative
damage induced by monocrotaline.
Khalid S Hashem,Kamal A Amin,
Mohamed S Hassan, El-Said T Awad,
International Journal of Nanomedicine 2011:6 143–149.
2- Deltamethrin-induced oxidative stress and biochemical changes in
tissues and blood of catfish (Clarias gariepinus): antioxidant defense and role
of
alpha-tocopherol
Kamal A Amin and Khalid S Hashem
BMC Veterinary Research 2012, 8:45
3-The prospective protective effect of selenium
nanoparticles against chromium-induced oxidative and cellular damage in rat
thyroid
Kamel MA Hassanin, Samraa H Abd El-Kawi, Khalid S Hashem
International Journal of Nanomedicine 2013:8
1713–1720.
4- Hepatoprotective
effects of vitamin C and micronized vitamin C against paracetamol induced
hepatotoxicity in rats: a comparative study
Kamel M. A. Hassanin, Khalid S. Hashem, Samraa H.
Abdel-Kawi. 2013. Int. J. Biochem.Biotechnol 2(7): 474-483.
5- A contribution towards milk enzymes, somatic cell count and
bacterial pathogens assosciated with subclinical mastitis cows milk.
Zeinhom, M. M. A, Abed, A.H. and Hashem,
K. S. 2013 Asuit vet. Med. J. (59): 138.
6- The effect of selenium on induced
ischemia / reperfusion injury in ovaries of female albino rat: a histological
and biochemical study. Dalia, H. abdel Aziz and Khalid S. Hashem. 2013. The Egyptian
journal of histology (36): 931.
7- Histological and biochemical studies on
the effect of vitamin E on sodium fluoride induced lung toxicity in adult
albino rats: Dalia, H.
Abdel Aziz, Samraa H. abdel kawi and Khalid S. Hashem. 2013: The
Egyptian journal of histology (36): 899.
8- Oxidative
hepatotoxicity effects of monocrotaline and its amelioration by lipoic acid,
S-adenosyl methionine and vitamin E. Kamal
Adel Amin, Khalid S. Hashem, Hessa M.
Al-muzafar and Eman M. Taha. Journal of Complementary and Integrative Medicine.
2014
(11)1: 35–41.
9- Selenium
nanoparticles increase the testicular antioxidant activity and spermatogenesis
in male rats as compared to ordinary selenium. Saber Abd-Allah and Khalid S. Hashem. International Journal of Advanced Research (2015).3 (1):
792-802.
10- Biochemical study on the impact of Nigella
sativa and virgin olive oils on cadmium-induced nephrotoxicity and
neurotoxicity in rats. Eman Taha Mohammed, Khalid
Shaban Hashem, Mahmoud Ramadan Abdel
Rheim. Journal of Investigational Biochemistry .(2013) 3 (2):70-77.
11- Cerium
oxide nanoparticles alleviate oxidative stress and decreases Nrf-2/HO-1 in
D-GALN/LPS induced hepatotoxicity. Reem M. Hashem a, Laila A. Rashd b, Khalid S.
Hashem, Hatem M. Soliman.
Biomedicine & Pharmacotherapy (2015). 73 : 80–86.
12- The
effect of aspirin nanoemulsion on TNFα and iNOSin gastric tissue in comparison
with conventional aspirin. Fatma Abd Elhalim Mahmoud, Khalid S. Hashem, Asmaa Mohammed M Hussein Elkelawy. International Journal of
Nanomedicine 2015:10.
13- The effect of high dietary fructose on the kidney of adult
albino rats and the role of curcumin supplementation. A biochemical and
histological study. Samraa H. Abdel-Kawi, Kamel
M.A. Hassanin, Khalid S. Hashem.
Beni-Suef University Journal
of applied sciences. 2015. http://dx.doi.org/ 10.1016/j.bjbas.2015.12.002
14- Mechanism of diethylhexylphthalate (DEHP)
induced testicular damage and of grape seed extract-induced protection in the
rat. Samraa H. Abdel-Kawi,
Khalid S. Hashem, Saber Abd-Allah (2016): Journal of food and chemical
toxicology. 90 (2016) 64-75.
Software:
- Microsoft office.
- Graphpad statistical programme.
References:
Pro. Dr. Mohamed A. Kandeil
Prof. Of Biochemistry and chemistry of
nutrition, Faculty of veterinary medicine, Beni-Suef Universirt, Beni-Suef,
Egypt.
Kandeil57@yahoo.com
Prof. Dr. Mohammed Samir Hassan
Prof. of Internal medicine, Feculty of vet.
Med.,
Beni Suef University, Benki Suef , Egypt.
Mohamedsamirhassan@yahoo.com
Dr. Kamel M abdallah Hassanin
Biochemistry department, Feculty of vet.
Med.,
Beni Suef University, Benki Suef , Egypt.
kamelmagha@hotmail.com
Master Title
Effects of some pollutant on some biochemical parameters in catfish obtained from Beni- Suef Governorate
Master Abstract
In the present investigation, eighty immature channel catfish with average weight 100 g ±5 were used. The fish were kept in glass aquaria one week before the beginning of the experiment for acclimation period. The water temperature about 22-23°c and pH level kept at 7.6±0.4. De-chlorinated tap water was supplied with oxygen by air pump. The fish were fed by commercial pelleted ration at 3% of body weight. The fish were divided into 7 groups. The first group reserved as a control. The second and third groups were exposed to Deltamethrin in a concentration 0.5µg /L and0.75 µg/ L for one week. The fourth group was exposed to mercuric chloride in a concentration of 140 µg/ L for one week. The fifth group was exposed to both Deltamethrin and mercuric chloride in a concentration 0.75 and 140 µg/ L respectively. The results revealed that ALT, AST, urea, creatinine and blood glucose were significantly increased while total protein, albumin were significantly decreased, GSH concentration and catalase activity of (liver, Kidneys and gills) also were significantly decreased in groups exposed to Deltamethrin and / or mercuric chloride when compared with control group while ALT, AST, urea, creatinine and blood glucose were significantly decreased, total protein, albumin concentration were significantly increased, GSH concentration and catalase activity of (liver, Kidneys and gills) also were significantly increased in groups exposed to vitamin E or group exposed to copper when compared with mixed polluted group and Deltamethrin group respectively.
PHD Title
Biochemical effects of some antioxidants on induced liver toxicity
PHD Abstract
Our work has been designed in two experiments as follows:
The first experiment was conducted to estimate the hepatic oxidative stress induced by injection of Monocrotaline (10 mg/kg body weight in 0.5 mL PBS, IP) and to determine the protective effects of cerium oxide nanoparticles (CeO2) (0.01µg/kg; 0.5 mL in PBS, IP) on days 1 and 3, a single dose of Mono on day 2 as described above, lipoic acid 30 mg/kg IP (days 1,3,5 and 7, 0.5 ml PBS) and a single dose of Mono on day 4 as described above, S- adenosyl methionine 20 mg/kg in four doses IP (days 1,2,4 and 5, 05 ml PBS) and a single dose of Mono day 3 as described above.
Our results show that, administration of these antioxidant substances (CeO2), lipoic acid, SAM and vitamin E caused a significant increase of hepatic reduced glutathione level (GSH), glutathione reductase and glutathione peroxidase and glutathione S- transferase activities when compared with the rat group received moncrotaline only. In addition, these substances also caused a significant decrease in liver catalase and super oxide dismutase activities as compared with monocrotaline injected group.
That's to say; administration of cerium oxide anonoparticles, lipoic acid, SAM and vitamin E can induce a sufficient protection against monocrotaline induced hepatic oxidative damage.
The second experiment was designed to estimate hepatic toxic effects of parcetamol (APAP), (600 mg/kg body weight every 24 hours for two doses) and to evaluate the protective effects of selenium nanoparticles injection in two different sizes (3- 5 nm and 10- 20 nm) as follow 0.5 mg Kg-1 I.P. body weight in two doses every 24 hours before and after APAP administration. Rats were scarified 24 hours after the last dose.
Our results show that, Se nanoparticles administration caused a significant decrease of serum liver enzymes (ALT, AST and ALP) when compared to paracetamol group. Moreover, Se nanoparticles modulated the decrease in serum albumin and total protein caused by APAP injection. Added to that, Se nanoparticles injection caused a significant increase in hepatic GSH contents, glutathione reductase activity as compare to APAP group. These effects were accompanied by a marked reduction in DNA fragmentation, lipid peroxidation (MDA), catalase and super oxide dismutase activities when compared to APAP injected group.
Finally, our biochemical results and oxidative biomarkers were confirmed by histopathological examination and electron microscope scanning which came in agreement with our biochemical results and inveterate the intracellular even distribution of the nanoparticles.