Basic Informations

C.V

Name:  Ahlam Hashem Owis Elwekeel

Title: Assistant lecturer in Pharmacognosy Department ,

Faculty of Pharmacy, Beni-Suef University, Egypt

E-mail: Ahlam_hasham2000@yahoo.com

Master Title

Tissue Culture and Biological Studies on Silybum marianum

Master Abstract

Callus and cell suspension cultures of Silybum marianum (L.) Gaertn. (Asteraceae) were established from in vitro germinated sterile plantlets. The cultures grew in Murashige and Skoog medium containing 1 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg l-1kinetin. A reversed phase-high performance liquid chromatography method for determination of flavanolignans in plant material was developed using an isocratic solvent system comprising acetonitrile and water containing 0.5% (v/v) phosphoric acid. Silychristin was the major flavanolignan produced by the cultured cells followed by silydianin. Elicitation of cultured S. marianum cells with 100 lg ml-1 yeast extract increased silychristin production from 0.11 to 0.23 mg g-1 fresh weight. Free radical scavenging activity was tested for the cultured cells using 1,1-diphenyl picryl hydrazyl (DPPH) radical. Extract prepared from the cultured cells of S. marianum showed 48% inhibition compared to 55% inhibition of the extract prepared from the fruits. Cytotoxic activity was tested using liver carcinoma cell line (HEPG2). Cultured cells and fruit extracts showed a significant cytotoxic activity of IC50 = 1.01 and 0.47 lg, respectively. Extract of S. marianum cultured cells ameliorated the adverse effects of carbon tetrachloride-induced hepatic injury in rats and

PHD Title

Phytochemical and Biological Studies on Some Plants Belonging to Family Crassulaceae Cultivated in Egypt

PHD Abstract

Crassulaceae with approximately 34 genera and 1400 species is one of the most important groups of succulent plants that are widely cultivated as ornamental because their leaves are aggregated into colorful rosettes. Crassula capitella and Crassula arborescens are two plants cultivated in Egypt and belonging to family crassulaceace were used for this study. This study include phytochemical investigation of the extracts of both plants which resulted in isolation and identification of 21 compounds belonging to different classes, all the compounds identified for the first time from the genus except Compound 8 [11-O-(4'-O-methyl galloyl ) bergenin] which previously isolated. Anti-arthritic activity of the total alcoholic extract of C. capitella and 11-O-(4'-O-methyl galloyl ) bergenin] were evaluated in complete Freund's adjuvant rats and the results showed that 20 mg/ kg of 11-O-(4'-O-methyl galloyl) bergenin showed potent anti-arthritic activity through lowering serum level of CRP, RF, anti-CCP, TNF-a, IL-1ß, IL-6, NO, COX-2, MPO, MDA when compared to methotrexate (2mg/kg). Anti-oxidant, antimicrobial and in- xvi vitro cyclooxygenase inhibitory activity of some of the isolated compounds of both plants were evaluated and results showed that quercetin and gallic acid were the potent anti-oxidant compared to ascorbic acid standard. Bergenin, isorhamentin, procatechuic, vanillic and 11-O-galloyl bergenin showed antimicrobial activity against some of the tested micro-organisms with MIC ranging from 12.5 to 100 µg/ ml. a, ß-amyrin mixture showed potent cyclooxygenase inhibitory activity with IC50 4.06 and 0.64 µM for COX-1 and COX-2 respectively. Also this study contain part deals with quantative determination of total phenolics and total flavonoids in both plants using Folin ciocalteau reagent and AlCl3 reagent respectively and results showed that C. capitella contain the higher percentage of phenolics and flavonoids while quantitation of different compounds in the methanol extract revealed that 11-O-(4'-O-methyl galloyl ) bergenin the major compound detected in C. capitella extract while quercetin was the major detected in C.arborescens extract. Key words: C. capitella, C. arborescens, 11-O-(4'-O-methyl galloyl) bergenin, anti-oxidant, anti-rheumatic, anti-microbial