Basic Informations

C.V

 

Cytology and Histology Department

Faculty of veterinary medicine

Beni-Suef University

Curriculum Vitae

========

- Name of Applicant :  Taghreed Mohamed Nabil Moustafa

- Current Academic Rank : Lecturer of Cytology and Histology

- Current University : Beni-Suef University

- Current Faculty : Faculty of Veterinary Medicine

- Current Department : Cytology and Histology Department

- Specialization :  Cytology and Histology.

                                                          PERSONAL DATA

- Date of Birth: 1/10 /1977                                             

 - Place of Birth: Beni-Suef Province

- Nationality: Egyptian

- Marital status: Married

- Address:  Beni-Suef, Egypt.

- Phone: 082 2214854   

            - Email: tagh_mhm@yahoo.com

- Work Address: Cytology and Histology Department, Faculty of Veterinary Medicine,          Beni-Suef, Egypt.

            Phone: 082 327982 / 082 322066                  Fax: 082 327982

- Marital Status:   Married                    

ACADEMIC QUALIFICATIONS

- Bachelor Degree of Veterinary Medical Sciences:

- Date of degree awarded:  May, 1999

- Faculty of Veterinary Medicine, Cairo University (Beni-Suef).

- Specialization: Veterinary Medical Sciences

- Master Degree of Veterinary Medical Sciences:

            - Date of degree awarded:    2/6/2004 

- Faculty of Veterinary Medicine, Cairo University (Beni-Suef).

- Specialization: Cytology and Histology

- Title of Thesis: pre and postnatal histological studies of the thyroid gland in rabbits.

- Ph. D degree of Veterinary Medical Sciences:

            - Date of degree awarded: 30/3/2009

- Faculty of Veterinary Medicine, Beni-Suef University.

- Specialization: Cytology and Histology

- Title of Thesis: Histological studies of the ovary and the uterus in relation to the     

  pituitary gland in rats.

COMPUTER USE

The applicant is highly aware by the following software:

DOS, WINWORD, EXCELL, INTERNET, POWER POINT

EMPLOYMENT HISTORY

- 19 February 2000 till 2 June 2004:

Demonstrator of Cytology and Histology, Cytology and Histology Department, Faculty of Veterinary Medicine (Beni-Suef), Cairo University.

- 2 June 2004 till 30 March 2009:

Assistant Lecturer of Cytology and Histology, Cytology and Histology Department, Faculty of Veterinary Medicine (Beni-Suef), Cairo University.

- 30 March 2009 till now:

- Lecturer of Cytology and Histology, Cytology and Histology Department, Faculty of Veterinary Medicine, Beni Suef University.

UNIVERSITY TEACHING

(I) Courses taught by applicant at university level:

- Cytology                                 - Respiratory system

- Epithelial tissue                       - Digestive system

- Connective tissue.                  - Urinary system.                  

- Blood                                      - Female genital system

- Muscular tissue                       - Male genital system.                                  

- Nervous tissue                        - Endocrine system

- Nervous system.                     - Integumentary system.

- Lymphatic system                   - Fish histology

- Cardiovascular system          - Avian histology    

  

RESEARCH AND CREATIVE ACTIVITIES

(I) Research articles that have been published in refereed journals:

 

1- Title: Role of Ginger Against the Reproductive Toxicity of Aluminium Chloride in Albino Male Rats

- Source: Reprod. Dom. Anim. 47: 335–343, (2012).

- Authors: Walaa, A. Moselhy¹, Nermeen, A. Helmy², B.R. Abdel-Halim³, Taghreed, M. Nabil⁴ and Marwa, I. Abdel-Hamid⁵

Foren. Med. Toxicol. Depart.¹, Physiol. Depart.²,  Theriogenol. Depart.³, Cytol. and Histol. Depart.⁴, Fac. Vet. Med., Beni-Suef Univ. and Biochem. Depart., Fac. Vet. Med., Cairo Univ.⁵

2- Title: Pre and Post-hatching Development of the Thymus Gland of Chicken

- Source:  Assiut Vet. Med. J. 60 (141): 200-208 (2014).

- Authors: Soliman,S.M., Mazher,K.M., Nabil,T.M., and Abdel Razik, A.H.

Cytology and Histology Depart., Faculty of Veterinary Medicine, Beni-Suef University

3- Title: Histological Changes of the Ampullary Epithelium of Cyclic Albino Rat Oviduct:   

  Light and Ultrastructure Examination

 -  Source: Global Veterinaria 14 (6): 903-912 (2015).

 -  Authors:     Taghreed M. Nabil

Department of Histology and Cytology, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.

4- Title: Prevention of Methotrexate-Induced Nephrotoxicity by Concomitant Administration of Garlic Aqueous Extract in Rat.

- Source: Turkish Journal of Medical Sciences 45: 507-516 (2015).

- Authors: Walaa AHMED¹, Amr ZAKI ², Taghred NABIL³

Clinical Pathology Depart., Faculty of Veterinary Medicine¹, Forensic Medicine and Clinical Toxicology Depart., Faculty of Medicine², Cytology and Histology Depart., Faculty of Veterinary Medicine³, Beni-Suef University, Egypt.

5- Title: Bisphenol A Toxicity in Adult Male Rats: Hematological, Biochemical and Histopathological Approach

 - Source: Global Veterinaria 14 (2): 228-238 (2015).

 -  Authors: Walaa M.S. Ahmed¹, Walaa A. Moselhy² and T.M. Nabil³

Clinical Pathology Depart¹, Toxicology & Forensic Medicine Depart.², Cytology & Histology Depart.³, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.

6- Title: Clinico-pathological Studies on the Regulatory Effect of Cinnamon on Metabolic Disorders in High Fructose-Fed Mice

 - Source: Egyptian journal of comparative pathology and clinical pathology 28 (1): 103-116 (2015).

 - Authors: Walaa M.S. Ahmed¹, Nermeen A. Helmy² and Taghreed M.Nabil³

Clinical Pathology Depart., ¹ Physiology Depart.,² Cytology and Histology Depart.,³, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.

7- Title: Cytomorphological and Morphometric Studies of Somatotrophs in the Pituitary Pars Distalis of Postnatal Female Albino Rats

- Source: Assiut Vet. Med. J 62 (148):123-131 (2016)

- Authors: Taghreed M. Nabil

Cytology and Histology Depart. , Faculty of Veterinary Medicine, Beni-Suef University

8 -Title: Effect of Atrazine and Glyphosate on the Reproductive System of Female Rats: Histological and Immunohistochemical Studies.

- Source: Assiut Vet. Med. J 62 (148):101-111 (2016)

- Authors: Walaa A Moselhy¹, Taghreed M Nabil², BR Abdel-Halim³ and Nermeen A Helmy⁴

Forensic Medicine and Toxicology Depart.¹ Cytology and Histology Depart.² Theriogenology Depart.³, Physiology Depart.⁴, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.

9- Title: Histological, Histochemical and Immunohistochemical Studies on the Paraventricular and Supraoptic nuclei of the Hypothalamus in the Adult New Zealand rabbits.

Source: Assiut Vet. Med. J. 61 (147): 179-190 (2015)

- Authors: SOLIMAN, SH. M.M.; MAZHER, KH.M; TAGHREED, M.N.; MOAWAD, U.K. and HASSAN, R.M.

Cytology and Histology Depart. , Faculty of Veterinary Medicine, Beni-Suef University.

10- Title: Histogenesis and Fate of the Fabricius bursa in chicken: Light and Electron study.

- Source: Presented in the 37^th Scientific Conference of the Egyptian Society of Histology & Cytology Faculty of Medicine, Alexandria University 12 /12/ 2013

- Authors: Soliman, S.M. Mazher, K.M, Nabil, T.M and Abdel-Razik, A.H.

Department of Cytology and Histology, Faculty of Veterinary Medicine, Beni-Suef University, Egypt

(III) Books authored by applicant (with others)

- Cytology & General Histology (Part I)

- Special Histology (Part II)

(IV) Supervision of Theses:

1- Title of Thesis: Identification and characterization of neurosecretory cells in rabbits during postnatal life.

- Degree: Ph. D degree (Histology)

- University Conferring the Degree: Faculty of Veterinary Medicine, Beni-Suef University.

- Date registration: 19- 12-2010 (approved)

2- Title of Thesis: Developmental stages and fate of some lymphatic organs in chickens

- Degree: Ph. D degree (Histology)

- University Conferring the Degree: Faculty of Veterinary Medicine, Beni-Suef University.

- Date registration: 23-5-2010 (approved)

3- Title of Thesis: Histogenesis of salivary glands in rabbits.

- Degree: Master degree (Histology)

- University Conferring the Degree: Faculty of Veterinary Medicine, Beni-Suef University.

- Date registration: 18- 12- 2011.

OTHER UNIVERSITY ACTIVITIES

1- Share in the 38^th   Scientific Conference of Egyptian Society of Histology and Cytology, Faculty of Medicine, Ain Shams University 25/12/2014.

2- Share in the 37^th   Scientific Conference of Egyptian Society of Histology and Cytology, Faculty of Medicine, Alexandria University 12/12/2013.

3- Share in the international Conference on Molecular Biology and Stem cells from 22-23 May 2013

4- Share in the 5^th International Congress of Egyptian Society for Environmental toxicology 2009.

5- Share in the training course of Egyptian society of Environmental Toxicology from 21- 22 October 2012

6- Share in the training course of Biostatistics and Data Analysis on April 2015.

       Head of department                                                                  Dean of Faculty

    

Prof. Dr. / Khaled Mohamed Mazher                                        Prof. Dr. / Magdy Elkady

Master Title

pre and postnatal histological studies of the thyroid gland in rabbits

Master Abstract

SUMMARY The results presented in this investigation elucidate the pre- and postnatal development of the thyroid gland of New Zealand white rabbit using 28 embryos and fetuses at different ages ranging from 10 – 30 days of prenatal life and 28 rabbits ranging between 3 days up till 5 years old. The collected specimens were serially and step-serially sectioned at 4-6 micrometers thick, and then stained with different histological stains for light and an electron microscopic examination. The bilateral thyroid primordium was recognized at 12 days old rabbit embryos, it appeared as bilateral masses of endodermal cells lying on either side of the tracheal anlage. The endodermal cells were arranged in the form of clumps or branching and anastomosing cords. By the development of the embryo, the cell cords grow due to active proliferation of the endodermal cells, during the growth, the cords were fragmented into shorter portions or clumps. From these clumps, the primitive follicles were developed at 14 days old rabbit embryo. These follicles appeared as small vesicle with narrow empty lumina. As the development proceeded the size of the lobe increased and the gland assumed a bilobed form connected by a narrow cellular isthmus at the 18th day of gestation. The connective tissue stroma begins as undifferentiated mesenchymal cells surrounding the thyroid primordium at 12 days old rabbit embryo. Then it differentiated into newly formed fibroblasts and reticular fibers as well as collagenic fibers at 14 days of pregnancy. The capsule increased in thickness with increasing of age of the fetus and was mainly formed of collagen, reticular fibers and connective tissue cells with some blood vessels and sinsoids. True follicles were seen at 20-22 days, while colloid material was seen in their lumina at 24 days old fetuses. Some new follicles were originated from the parent follicles by budding as well as from the ultimobranchial cyst. The follicles increased in size with aging and took a characteristic distribution within the thyroid lobule; the small follicles were centrally located which were more active than the large follicles which located peripherally. In very active follicles, the colloid was uptaken and re-entered the follicular cells by the process of micropinocytosis and appeared in the follicular cytoplasm as colloid droplets. Degenerative changes appeared in the follicular cells of some thyroid follicles especially in senile ages. The first appearance of the colloid material in the lumen of some follicles specially the central ones at the 24th day of gestation but the majority of the follicles still devoid of colloid till the late stage of pregnancy and increased gradually as well as with vacuolations after birth (at the postnatal life) and reached the peak in the adult rabbits but the colloid and the vacuolations decreased gradually with proceeding age till all the follicular lumens lose their colloid and appeared empty in case of highly senile rabbits. The follicular colloid was PAS positive and fuchsinophilic or light green (+ve) or showed double staining reaction as well as giving positive reaction with touilidine blue stain but negative reaction with Alcian blue stain. The primordia of the ultimobranchial body was observed beside the thyroid lobe at 20 days of gestation as a compact cell mass and then became embedded into it at 22 day of gestation and then transformed into cystic structures which shared in the formation of the thyroid follicles by budding at the late stage of pregnancy and after birth. They were lined by simple cuboidal or columnar epithelium and sometimes appeared stratified. Some large cells with light staining cytoplasm were observed in the cyst wall. The lumina of some cysts contained colloid-like material. The ultimobranchial cysts persisted in the postnatal life up to senile age but some of them showed degenerative changes and cell debris as well as macrophages appear in their colloid like material. Few light cells or parafollicular cells were observed firstly at 12 days old embryos scattered between the cell cords and clumps. They were easily observed at 25 days old fetus either singly within the follicular epithelium or in small clusters in the interfollicular spaces as well as between the undifferentiated cell cords and clumps. They were large spherical, oval or polyhedral with lightly stained cytoplasm and large nuclei. Mitotic cell divisions were seen in light cells especially in prenatal life. Some of C-cells migrated as a collection of light cells and directed towards the follicles through the interfollicular connective tissue. The number of C-cell will be decreased in senile ages and some of them showed degenerative changes. With electron microscopical examination in the prenatal stages the follicular cells appeared in hypoactive state which indicated by the ill-developed cytoplasmic organelles except scattered numbers of RER with dilated cisternae as well as rounded mitochondria. But, with increasing the fetal age till the late stage of pregnancy, some of follicular cells were somewhat active due to presence of euchromatic nucleus as well as most of cytoplasmic organelles were well developed and distinct. The maximum rate of activity of the follicular cells appeared after birth especially in adult rabbits because of all cytoplasmic organelles which responsible for glycoprotein synthesis and secretion were numerous and well-developed as RER, Mitochondria and Golgi apparatus as well as free ribosomes and lysosomes which were seen fused with the secretory vesicles. The apical parts of the adjacent follicular cells sealed by tight junction, while the lateral borders showed desmosomes The apical plasma membrane bordering the follicular lumen exhibited numerous short finger-like projections or microvilli which extending into light-electron dense colloid. In senile rabbits, the follicular cells appeared in hypoactive state and most of cytoplasmic organelles and the nucleus showed degenerative changes. In addition, the parafollicular cells were examined ultrastructurally which appeared in hypoactive state in the early prenatal stages, but their activity began at the late stage of pregnancy and increased after birth which showed well-developed RER, numerous free ribosomes as well as considerable amount of electron-dense bodies. In senile rabbits many degenerative changes were observed in the nucleus and the cytoplasmic organelles of the parafollicular cells. No clear sex difference was observed.

PHD Title

Histological studies of the ovary and the uterus in relation to the pituitary gland in rats.

PHD Abstract

SUMMARY The fundamental aim of this investigation is to elucidate the histological and the electron microscopical structure of four types of hypophyseal cells including folliculotrophs, luteotrophs, lactotrophs and thyrotrophs and their relation to the histomorphological and electron microscopical changes in the corresponding ovaries and endometrium. Our study has been carried out on seventy eight female albino rats (Rattus norvegcus albinos) classified into three ages; immature, mature and senile ages. The mature rats subdivided into five groups according to different phases of estrous cycle that is to say early proestrus, late proestrus, estrus, metestrus and diestrus. The collected specimens (pars distalis, ovaries & uteri) were embedded in paraffin, serially and stepserially sectioned at 4-6 micrometers thick, then stained with different histological and histochemical dyes to be prepared for light microscopical examination. Some specimens were, also, treated to be examined by electron microscope. The pars distalis was surrounded by a thin delicate fibrous capsule and contained fine interstitial tissue in immature ages. They were increased in thickness and density with advancing ages. Folliculotrophs couldn’t be differentiated from luteotrophs up to 2nd week of neonatal rats. They were stained similarly. The cells were located peripherally in a small- sized PAS +ve area (sex zone) which became enlarged with advanced age. The differentiation was noticed at 22-days-old rat. As the folliculotrophs were polymorphic in shape and contained numerous polymorphic secretory granules stained purplish – violet, the luteotrophs were PAS+ ve oval or irregular cells containing uniform secretory granules. Lactotrophs were easily to be identified at 22-day -old rat. They appeared in different forms with an orangeophilic cytoplasm containing few, coarse and polymorphic secretory granules. The few pale bluish thyrotrophs were noticed at 7-day-old rats .They were increased with advanced ages and appeared angular in shape with a deeply bluish cytoplasm. The ovary at 7- day - old rats were covered by simple cuboidal epithelium with stratifications in some areas and housing naked- oocytes. With advanced ages, the surface epithelium was changed to simple squamous and the parenchyma was composed of numerous primordial polyovular follicles. With advanced ages, numerous primary and secondary follicles were located more centrally. No coropora lutea were seen till the 3rd week. The ovarian stroma appeared as epithelioid cells at 22-days- old rat containing lipid droplets ,mitochondria and RER. At the first week, the uterine lumen was regular, smooth and lined with simple columnar epithelium showing some stratification. While at the 2nd week of life, epithelial invaginations as well as small –sized tubular were the fore-runners of endometrial glands. Further growth was recorded at the third week in the form of increase in thickness and the glands became highly folded and numerous. During early proestrus, folliculotrophs were numerous and sparsely- granulated or degranulated with dilated cisternea of RER and filamentous mitochondria. While at late proestrus, they appeared in exhausted phase with clear cytoplasm. Strongly PAS+ve luteotrophs were present in early proestrus containing numerous electron – dense granules. While at late proestrus, some of luteotrophs were sparsely- granulated or degranulated. Most of lactotrophs in early proestrus were granulated and contained few organelles while they became sparsely granulated and degranulated with well- developed organelles in late proestrus The ovary during early proestrus showed different developmental stages of ovarian follicles. Mitotic divisions were seen in the granulosa cells. Also, regressed corpora lutea of previous cycle were seen. At late proestrus, mature ovarian follicles with large antra were present. Their granulosa cells contained lipid droplets and well- developed organelles. The uterine surface epithelium in early proestrus was formed of high columnar cells showing mitotic activity. The endometrial glands were lined with low columnar cells containing PAS+ve granules. At late proestrus, progressive increase in the height of the columnar cells of both surface and glandular epithelium occurred as well as PAS+ve secretory material appeared in their lumina.The endometrial stroma was loose, highly oedematous and showed congested blood vessels. During estrus phase, folliculotrophs were regranulated and few well-developed organelles were present. Most of luteotrophs were sparsely- granulated and their cytoplasm had few secretory granules, dilated cisternea of RER and intra cisternal granules. Some of lactotrophs were granulated and others sparsely- granulated. Thyrotrophs were active and sparsely- granulated in both pro-andestrous phases. Recently formed corpora lutea with central lumina containing clotted blood were present in the ovary during the estrus phase beside many antral atretic follicles and old regressed coropora lutea. During the estrus phase, marked decrease in the height of the surface and glandular epithelia of the uteri were seen. In addition, acidophilic secretory material mixed with desquamated and inflammatory cells were present in their lumina. In luteal phase, folliculotrophs were heavily granulated and most of cytoplasmic organelles were ill- observed. On the other hand, luteotrophs were hypertrophied, sparsely- granulated and had well- developed organelles. No histological changes were observed in lactotrophs. Thyrotrophs became few in number and regranulated. The ovary in luteal phase showed well- developed corpora lutea. Their granulosa luteocytes contained lipid droplets and well- developed organelles. The columnar cells of the uterine epithelium carried few, short microvilli and their cytoplasm contained lipid droplets which increased in diestrus. In addition, further deep down growths of endometrial glands were seen. They became highly coiled and branched in diestrus. In senile ages, most of secretory cells of the pars distalis became further fewer, smaller and showed degenerative changes. The ovary of 1.5 years- old rat were decreased in size and the interstitial tissue was increased on the expense of the ovarian follicles. While at 2 years - old many atretic follicles and ovarian cysts were present with disappearance of corpora lutea. The uterine lumen in senile rats became slightly regular and smooth. Few, tubular, small- sized endometrial glands were scattered in the highly fibrous stroma.