Basic Informations

C.V

Personal information

Name: Maimana Ahmed Magdy Abdelaziz

Nationality: Egyptian
Birth date: 19/5/1985. Gender: Female.
Address: Beni-suef,Egypt

Email: maimanamagdy@yahoo.com Mobile: 002 01006773868

Education and Professional Qualifications

University Degree            : Bachelor in pharmaceutical sciences.
University                        : Beni-Suef University.
Grade                              : Excellent with honor.
Graduation Year                    : 2007.
After Graduation studies:   * Premaster study in analytical chemistry.

* Presentation Skills course.

Scientific Publication :

1) drug i nventio n today 5 ( 2013 ) 139el47 " Stability indicating spectrophotometric methods

for determination of bumadizone in the presence of its alkaline degradation product"

2) Biomed. Chromatogr. 2012; 26: 1143–1149 " Simultaneous HPTLC and RP-HPLC methods for

determination of bumadizone in the presence of its alkaline-induced degradation product

3) J.Chem.Soc.Pak., Vol. 36, No. 6, 2014 "Novel Spectrophotometric Methods for Determination o of Salicylamide and Ascorbic acid in their binary mixture.

4) Pharm Anal Acta Volume 2 • Issue 9 • 1000140 "Simultaneous Determination of Paracetamol and Diphenhydramine Hydrochloride in Presence of Paracetamol Degradation Product.

5) Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 132 (2014) 655–662 "Kinetic study and mechanism of Niclosamide degradation

Training & Work Experience

Training Experience :4 years training & working in 2 community pharmacies.

Work Experience : experience as:
*Demonstrator of Pharmaceutical Analytical Chemistry (Preparing labs, Chemical
testing and preparing practical exam) in Faculty of Pharmacy, Beni-Suef University.

*Assistant lecturer of Pharmaceutical Analytical Chemistry (Preparing labs, Chemical
testing and preparing practical exam) in Faculty of Pharmacy, Beni-Suef University.

*Researcher as Analytical Chemist for analysis & Q.C for pure drugs and
Pharmaceutical Formulations.

Skills

Computer skills

WINDOWS 8
MS. OFFICE (WORD, EXCEL&POWERPOINT)

Language skills

Arabic : Mother Tongue. English: Good command.

Interests

Reading & Internet.
Help others.
Human Development science.

C.V PDF

Master Title

Analysis of some amide group and/or ester group containing pharmaceutical drugs

Master Abstract

Abstract "Analysis of some amide group and/or ester group containing pharmaceutical drugs" This thesis consists of three parts, each part includes an introduction, literature review and descriptive experimental work for the studied drugs; in addition to refernces and a summary in Arabic. Part I: STABILITY INDICATING METHODS FOR DETERMINATION OF BUMADIZONE IN PRESENCE OF ITS DEGRADATION PRODUCT This part includes: Introduction and Literature Review This introduction describes the pharmacological action of Bumadizone, its chemical structure, physical properties and review of the published methods developed for its analysis. Section (A): Stability Indicating First Derivative and First Derivative of Ratio Spectra Spectrophotometric Methods for Determination of Bumadizone In this section, a first derivative and first derivative of ratio spectra spectrophotometric methods were applied to determine intact Bumadizone in presence of its degradation product in methanol at 245.5 nm for 1D method and at 242.6, 260 and 274 nm for 1DD method. A linear correlation was obtained in the range of 6-26 µg mL-1 of Bumadizone with mean percentage recovery of 100.43 ± 0.88 for 1D method while 1DD method showed good linearity at 242.6, 260 and 274 nm in the range of 6-20 µg mL-1 with the mean percentage recoveries 100.13±1.21 , 100.18±1.29 and 99.62±0.63 at 242,6, 260 and 274 nm respectively. The proposed methods retained their accuracy in the presence of up to 70 % of the degradation product at 245.5 nm, while up to 60% of the degradation product at 242.6 and 274 nm, and up to 40% of the degradation product at 260 nm. The proposed methods were applied for the determination of Bumadizone in its pharmaceutical formulation where the validity was further assessed by applying the standard addition technique. Section (B): Stability Indicating Isoabsorptive Spectrophotometric Method for Determination of Bumadizone In this section Isoabsorptive spectrophotometric method was proposed for determination of Bumadizone in presence of its degradation product, while the degradation product can be measured at its ? max 320 nm and Bumadizone can be measured at ? 242.2 nm (Aiso) using 0.1 M HCl as a solvent. The suggested method was applied for determination of Bumadizone in its pharmaceutical formulation and the validity of the method was further assessed by applying the standard addition technique. Section (C): Stability Indicating Ratio-Subtraction Spectrophotometric Method for Determination of Bumadizone In this section the suggested Ratio-Subtraction spectrophotometric method was applied for determination of Bumadizone in presence of its degradation product, while the abosorbance was measured at 236.6 nm using 10 µg mL-1 of the degradation product as a divisor and methanol as a solvent. The suggested method was applied for determination of Bumadizone in its pharmaceutical formulation and the validity of the method was further assessed by applying the standard addition technique. Section (D): Stability Indicating Multivariate Calibration Method for Determination of Bumadizone Two chemometric techniques; principal component regression (PCR) and partial least squares (PLS) were used for the determination of Bumadizone in presence of its degradation product Training set consisted of fifteen mixtures containing different ratios of Bumadizone and its degradation product was used for construction of the models. The selectivity of the proposed method was checked using laboratory prepared mixtures (validation set consisted of nine mixtures). Satisfactory results were obtained upon applying the proposed methods for the analysis of Bumadizone in its pharmaceutical formulation. Applying standard addition technique assessed the validity of the suggested methods. Section (E): Stability Indicating HPTLC and RP-HPLC Methods for Determination of Bumadizone In this section two chromatographic methods were used for separation of the drug from its degradation product. HPTLC-densitometric method was applied by using hexane :ethylacetate :glacial acetic acid (8:2:0.2, by volume) as a developing system. The bands measured quantitatively at 240 nm, while RP-HPLC was achieved by using methanol :water : acetonitrile (20:30:50, by volume) as a mobile phase, adjusted pH of the solution to 3.5 using phosphoric acid at a flow rate 2 mL min-1.and UV detection at 235 nm. The suitability of the proposed chromatographic methods was ascertained by the determination of system suitability parameters of the separated components.The suggested methods could be considered as a stability-indicating method as they could determine the drug in presence of upto 70% of its degradation product for HPTLC method and upto 90% of its degradation product for RP-HPLC method. The suggested methods were successfully applied for the determination of Bumadizone in its pharmaceutical formulation. Part II: DETERMINATION OF PARACETAMOL AND DIPHENHYDRAMINE HYDROCHLORIDE IN BINARY MIXTURE AND IN PRESENCE OF PARACETAMOL DEGRADATION PRODUCT This part includes: Introduction and Literature Review This introduction comprises a brief idea about the structure, properties, stability and different methods for the analysis of Paracetamol and Diphenhydramine Hydrochloride either alone or in their binary mixture. Section (A): First Derivative and First Derivative of Ratio Spectra Spectrophotometric Methods for Determination of Paracetamol and Diphenhydramine Hydrochloride in presence of Paracetamol degradation product In this section, 1D and 1DD spectrophotometric methods were applied to determine Paracetamol and Diphenhydramine Hydrochloride in presence of Paracetamol degradation product, where Paracetamol was determined at 264.5 nm using 1D method and methanol as solvent, while 1DD method used for determination of Diphenhydramine Hydrochloride using 10 µg mL-1 of Paracetamol degradation product as a divisor and methanol as a solvent. The linearity range of Paracetamol and Diphenhydramine Hydrochloride were 2-12 µg mL-1 and 5-18 µg mL-1 respectively. Paracetamol and Diphenhydramine Hydrochloride were determined successfully in laboratory prepared mixtures using the proposed method. The validity of the suggested methods was checked by the analysis of Paracetamol and Diphenhydramine Hydrochloride in their pharmaceutical formulation and the standard addition technique assessed this validity. Section (B): Multivariate Calibration Technique for Determination of Paracetamol and Diphenhydramine Hydrochloride in presence of Paracetamol degradation product Two chemometric techniques; principal component regression (PCR) and partial least squares (PLS) were used for simultaneous determination of Paracetamol and Diphenhydramine Hydrochloride in presence of Paracetamol degradation product. Training set consisted of thirteen mixtures of Paracetamol and Diphenhydramine Hydrochloride in presence of Paracetamol degradation product containing different ratios, was used for construction of the models in the spectral region 220-340 nm. Eeight mixtures were used as validation set; the proposed method was successfully applied for determination of the binary mixture in their pharmaceutical formulation. Results obtained by the proposed method were statistically compared with that obtained by the official method indicating no significant difference between them. Section (C): HPTLC Method for Determination of Paracetamol and Diphenhydramine Hydrochloride in presence of Paracetamol degradation product In this section, a simple and accurate TLC-densitometric method was suggested for simultaneous determination of Paracetamol and Diphenhydramine Hydrochloride in presence of Paracetamol degradation product in bulk powder and in pharmaceutical formulation. The method is based on the difference in Rf values of the cited drugs. Satisfactory separation was obtained by using chloroform : ethyl acetate : ammonia solution (4:6:0.2 by volume) as a developing system. The Rf values are 0.13, 0.5 and 0.3 for Paracetamol, Diphenhydramine Hydrochloride and P-aminophenol respectively. The bands were scanned at 220 nm giving maximum sensitivity for the drugs. The proposed method was successfully applied for determination of the binary mixture in their pharmaceutical formulation and validity was assessed by applying standard addition technique. Part III: SPECTROFLUORIMETRIC AND SPECTROPHOTOMETRIC DETERMINATION OF GLIQUIDONE This part includes: Introduction and Literature Review This introduction describes the pharmacological action of Gliquidone, its chemical structure, physical properties and review of the published methods developed for its analysis. Section (A): Spectrofluorimetric Determination of Gliquidone In this section the native fluorescence of Gliquidone was measured at 404 nm upon excitation at 311 nm. The linearity range was 0.05-0.45 µg mL-1 with mean percentage recovery 100.00±0.92. The high sensitivity attained by the proposed method allows the determination of Gliquidone in spiked human plasma. The suggested method was used for determination of Gliquidone in its pharmaceutical formulation, where satisfactory results were obtained. The validity of the proposed method was further assessed by applying standard addition technique. Statistical analysis of the results proved no siginifican difference compared with the reported method. Section (B): Spectrophotometric Determination of Gliquidone Using 7,7,8,8,-Tetracyanoquinodimethane (TCNQ) This method based on formation of charge transfer complex between the Gliquidone and the reagent, where the drug acted as an electron-donor and TCNQ acted as electron-acceptor. The obtained complex has an absorption maximum at 745.5 nm and the pure drug was determined in the linearity range 0.2-2 mg mL-1. Factors affecting the reaction condition and drug sensitivity were investigated. The suggested method was successfully applied for determination of Gliquidone in pure form and in pharmaceutical formulation and the results obtained were statistically compared with the reported one.

Master Abstract PDF

PHD Title

Analytical Study of Some Pharmaceutical Compounds Containing Carbonyl Group

PHD Abstract

Abstract This thesis consists of three parts, each part includes an introduction, literature review and descriptive experimental work for the studied drugs; in addition to references and a summary in Arabic. Part I: STABILITY INDICATING METHODS FOR DETRMINATION OF NICLOSAMIDE IN PRESENCE OF ITS DEGRADATION PRODUCTS This part includes: Introduction and Literature Review This introduction describes the pharmacological action of Niclosamide, its chemical structure, physical properties and review of the published methods developed for its analysis. Section (A): Stability Indicating Double Divisor-Ratio Spectra Spectrophotometric Method for Determination of Niclosamide With Kinetic Study of its Alkaline Degradation In this section, double divisor ratio spectra spectrophotometric method was applied to determine intact Niclosamide in presence of its degradation products without prior separation. The absorption spectra of the solutions prepared of different concentrations of pure Niclosamide and the ternary mixture were recorded and divided by the absorption spectrum of the mixed solution of CNA and CSA with equal concentration as a double divisor. First derivative of these ratio spectra were obtained and then the peak amplitudes values at 243 nm were plotted against the corresponding concentrations of Niclosamide to obtain the calibration curve with the corresponding regression equation. The double divisor ratio spectra spectrophotometric method was used to determine the order of the alkaline degradation rate of Niclosamide by following the decrease in the concentration of the drug within 6 hours at one hour time interval. The kinetic of Niclosamide alkaline degradation was found to follow pseudo-first order reaction. Section (B): Stability Indicating Modified Ratio Difference Spectrophotometric Method for Determination of Niclosamide In this section modified ratio difference spectrophotometric method was proposed for determination of Niclosamide in presence of its degradation products, Different concentrations of Niclosamide have been divided by standard spectrum of 20 µg/mL CNA (as a divisor) to obtain division spectra, then the difference in the amplitude values at 244 and 283 nm were measured (at which CNA was constant and CSA has equal amplitudes). The suggested method was applied for determination of Niclosamide in its pharmaceutical formulations and the validity of the method was Furfuralther assessed by applying the standard addition technique. Section (C): Stability Indicating Mean Centering of Ratio Spectra Spectrophotometric Method for Determination of Niclosamide In this method the mean centered ratio spectra amplitudes at 234 nm was used for quantitation of Niclosamide in presence of its degradation products. The suggested method was applied for determination of Niclosamide in its pure form and pharmaceutical formulations. The validity of the method was Furfuralther assessed by applying the standard addition technique. Section (D): Stability Indicating Multivariate Calibration Method for Determination of Niclosamide and Its Degradation Products (Related Substances) Two chemometric techniques; principal component regression (PCR) and partial least squares (PLS) were used for the determination of Niclosamide in presence of its degradation products. Training set consisted of 25 mixtures containing different ratios of Niclosamide and its degradation products was used for construction of the models. The selectivity of the proposed method was checked using laboratory prepared mixtures (validation set consisted of eight mixtures). Satisfactory results were obtained upon applying the proposed methods for the analysis of Niclosamide in its pharmaceutical formulations. Section (E): Stability Indicating TLC-Densitometric Method for Determination of Niclosamide and Its Degradation Products (Related Substances) In this section TLC-densitometric method was used for separation of the drug from its degradation products. The proposed method was applied by using benzene: ethyl acetate: methanol: triethylamine (9:1:1:0.1, by volume) as a developing system. The bands measured quantitatively at 230 nm. The suitability of the proposed chromatographic method was Ascorbic Acidertained by the determination of system suitability parameters of the separated components. The suggested methods was successfully applied for the determination of Niclosamide in its pharmaceutical formulations. Section (F): Stability Indicating and RP-HPLC-DAD Method for Determination of Niclosamide and Its Degradation Products (Related Substances) In this section RP-HPLC-DAD method was used for determination of Niclosamide and its degradation products. RP-HPLC was achieved by using water (adjusted to pH 3.5 using glacial acetic acid) : acetonitrile (30:70, v/v) as a mobile phase, at a flow rate 1 mL/min, and DAD detection at 230 nm. The suitability of the proposed chromatographic method was Ascorbic Acidertained by the determination of system suitability parameters of the separated components. The suggested method was successfully applied for the determination of Niclosamide in its pharmaceutical formulations. Part II: DETERMINATION OF HYDROXYZINE HYDROCHLORIDE, EPHEDRINE HYDROCHLORIDE AND THEOPHYLLINE This part includes: Introduction and Literature Review This introduction comprises a brief idea about the structure, properties and different methods for the analysis of Theophylline either alone or in its ternary mixture with Hydroxyzine Hydrochloride and Ephedrine Hydrochloride. Section (A): Zero Order Combined with Manipulating Ratio Spectrophotometric Methods for Simultaneous Determination of Hydroxyzine Hydrochloride, Ephedrine Hydrochloride and Theophylline In this work a newly developed spectrophotometric method has been established utilizing ratio-subtraction and 3D derivative spectrophotometry to resolve the overlap between the studied components. Theophylline can be determined directly at its ? max 272 nm, (without any interference from Ephedrine Hydrochloride and Hydroxyzine Hydrochloride). For determination of Hydroxyzine Hydrochloride, different concentration of Hydroxyzine Hydrochloride were divided by the standard spectrum of 22 µg/mL Theophylline, then the peak amplitude of the obtained spectra were measured at 234.2 nm, while for determination of Ephedrine Hydrochoride, different concentrations of Ephedrine Hydrochloride were divided by the standard spectrum of 22 µg/mL Theophylline, then derivatized using ??=4 and scaling factor 100 to obtain 3D spectra and measuring the peak amplitudes at 222 nm for 3D spectra of Ephedrine Hydrochloride. The proposed method was successfully applied for determination of Hydroxyzine Hydrochloride, Ephedrine Hydrochloride and Theophylline in their pharmaceutical formulation (Bronchaline® tablets). Section (B): Multivariate Calibration Method for Simultaneous Determination of Hydroxyzine Hydrochloride, Ephedrine Hydrochloride and Theophylline Two chemometric techniques; principal component regression (PCR) and partial least squares (PLS) were used for simultaneous determination of Theophylline, Hydroxyzine Hydrochloride and Ephedrine Hydrochloride. Training set consisted of 25 mixtures of Theophylline, Hydroxyzine Hydrochloride and Ephedrine Hydrochloride, containing different ratios was used for construction of the models in the spectral region 210-230 nm. Seven mixtures were used as validation set; the proposed method was successfully applied for determination of the ternary mixture in their pharmaceutical formulation. Results obtained by the proposed method were statistically compared with that obtained by the reported method indicating no significant difference between them. Section (C): TLC-Densitometric Method for Simultaneous Determination of Hydroxyzine Hydrochloride, Ephedrine Hydrochloride and Theophylline In this section, a simple and accurate TLC-densitometric method was suggested for simultaneous determination of Theophylline, Hydroxyzine Hydrochloride and Ephedrine Hydrochloride in bulk powder and in pharmaceutical formulation. The method is based on the difference in Rf values of the cited drugs. Satisfactory separation was obtained by using chloroform: ammonium acetate buffer (9.5: 0.5, v/v) adjusting to pH 6.5 using ammonia solution as a developing system. The bands were scanned at 220 nm giving maximum sensitivity for the drugs. The proposed method was successfully applied for determination of the ternary mixture in their pharmaceutical formulation and validity was assessed by applying standard addition technique. Part III: DETERMINATION OF SALICYLIC ACIDICYLAMIDE AND ASCORBIC ACIDORBIC ACID IN THEIR BINARY MIXTURE AND IN PRESENCE OF THEIR IMPURITIES This part includes: Introduction and Literature Review This introduction describes the pharmacological action of Salicylic Acidicylamide, its chemical structure, physical properties and review of the published methods developed for it either alone or in presence of its binary mixture with Ascorbic Acidorbic acid. Section (A): Area Under Curve and Dual Wavelength Spectrophotometric Methods for Simultaneous Determination of Salicylic Acidicylamide and Ascorbic Acidorbic acid in Their Binary Mixture In this section, the Area under curve and dual wavelength spectrophotometric methods were developed for determination of Salicylic Acidicylamide and Asocrbic Acid in their binary mixture. In the simultaneous equations using AUC method, the absorptivity (Y) values of each of the two drugs were determined at the selected wavelength ranges, 225-245 nm (?1-?2) and 265-285 nm (?3-?4). By applying Cramer's rule, concentrations of Salicylic Acidicylamide and Ascorbic Acidorbic Acid can be obtained. While The dual wavelength spectrophotometric method, the absorbance values of Ascorbic Acidorbic Acid are the same at 240.4 and 286.4 nm therefore these two wavelengths were selected for determination of Salicylic Acidicylamide. The same for the two wavelengths 249.8 and 285.8 nm, the absorbance values of Salicylic Acidicylamide are the same, hence those two wavelengths were selected for determination of Ascorbic Acidorbic Acid. The suggested methods were used for determination of Salicylic Acidicylamide and Ascorbic Acidorbic acid in their pharmaceutical formulation, where satisfactory results were obtained. Section (B): Derivative Spectrophotometric Method Combined With Isoabsorptive Point or Ratio Subtraction Spectrophotometric Methods for Determination of Salicylamide and Ascorbic Acid in Their Binary mixture In this section Salicylamide can be measured by 1D spectra at which (Ascorbic Acid showed zero-crossing) at 315.4 nm, using ??= 4 nm and scaling factor=10. While determination of Asocrbic Acid could be achieved either by isoabsorptive point or by ratio-subtraction spectrophotometric method. The isoabsorptive point spectrophotometric method was applied by measuring the absorbance value at the chosen isoabsorptive point, the total concentration of the mixture could be calculated. By applying the suggested procedure the absorbance at 246.4 nm (Aiso1) and 287 nm (Aiso2) for Ascorbic Acid was obtained over different concentrations. Then the concentration of Ascorbic Acid could be calculated by subtraction of Salicylamide concentration from total mixture concentration. The ratio subtraction spectrophotometric method, the spectra of mixtures containing different concentration of Salicylamide and Ascorbic Acid were divided by the spectrum of 10 µg/mL of Salicylamide as a divisor. The amplitude value in the plateau region at ? above 304 nm was subtracted from the spectra of the divided mixtures; the obtained spectra were then multiplied by the spectrum of the divisor. Finally, Ascorbic Acid concentrations in laboratory prepared mixtures were measured from the last spectra obtained at its ?max= 265.4 nm. The suggested methods were successfully applied for determination of Salicylamide and Ascorbic Acid in their pure forms and in pharmaceutical formulation and the results obtained were statistically compared with the reported one. Section (C): Double Divisor-Ratio Spectra Spectrophotometric Method for Determination of Salicylamide and Ascorbic Acid in presence of Their Impurities In this section, double divisor ratio spectra spectrophotometric method has been successfully applied for determination of Salicylamide and Ascorbic Acid in presence of their potential impurities Salicylic Acid and Furfural, respectively. 1D spectra were obtained using absorption spectrum of a mixture containing 5 µg/mL of each of Salicylic Acid and Furfural as a divisor. Salicylamide and Ascorbic Acid were quantitatively determined at 248 and 242.8 nm, respectively. The proposed method was successfully applied for determination of the suggested drugs in their pharmaceutical formulation; satisfactory results were obtained. The results obtained by the proposed method were compared to the reported HPLC method; they showed no significant difference regarding accuracy and precision. Section (D): Multivariate Calibration Method for Determination of Salicylamide and Ascorbic Acid in presence of Their Impurities Multivariate calibration models, such as PCR and PLS have been applied for determination of Salicylamide, Ascorbic Acid, Salicylic Acid and Furfural. Training set of 25 mixtures containing different ratios of the four proposed components was used for construction of the two models, Six mixtures were used as validation set. Satisfactory results were obtained on applying the proposed methods for the analysis of Salicylamide and Ascorbic Acid in their pharmaceutical formulation. Section (E): RP-HPLC-DAD Method for Determination of Salicylamide and Ascorbic Acid in Presence of Their Impurities A precise, accurate and specific RP- HPLC-DAD method was developed in this section. The separation was carried out on C18 column using 0.1% sodium lauryl sulfate aqueous solution (adjusted to pH 4 using orthophosphoric acid): methanol (45:55, v/v), at a flow rate 1 mL/min. The eluted compounds were monitored at 240 nm. The method is comparable with the reported HPLC method.

PHD Abstract PDF