Basic Informations
C.V
Curriculum Vitae
Name: Ibrahim Ahmed Naguib
Nationality: Egyptian Date of birth: Nov 9th, 1979
Current position: Associate Professor of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.
Home address: Flat 6, 2nd floor, El-Sirag Palace, Hatem Roshdy st., Beni-Suef, Egypt.
Office address: Faculty of Pharmacy, Beni-Suef University, Egypt.
Cellular phone: +2 01116813202
E-mail address: ibrahimnaguib1979@yahoo.com; ibrahim.yaseeen@pharm.bsu.edu.eg
University website: www.bsu.edu.eg; http://www.bsu.edu.eg/profresFeild.aspx?profid=322; http://www.pharm.bsu.edu.eg/
ORCID ID: 0000-0002-5923-1466
Scopus Author ID: 13610504900
Google scholar account: https://scholar.google.com/citations?view_op=list_works&hl=en&gmla=AJsN-F5E7Z-1Dl3C0EodHffikc9ytKPLSkSRgZlY4pN6KjlnmWFtCbfrV-l5sz9lIEiKSPXsfj2huC9nin5ddA9dzH2obG6QfPfOtgerJFQLaRcM0bAOtuA&user=DVgL8jwAAAAJ
Research gate account:
https://www.researchgate.net/profile/Ibrahim_Naguib?ev=hdr_xprf
Linkedin account:
https://eg.linkedin.com/in/ibrahim-naguib-058688173?trk=people-guest_profile-result-card_result-card_full-click
Educational history:
- PhD degree of pharmaceutical sciences (Analytical Chemistry), Faculty of Pharmacy, Beni-Suef University, December 2009 (with 2 years study experience in Bristol, UK).
- Master degree of pharmaceutical sciences (Analytical Chemistry), Faculty of Pharmacy, Cairo University, January 2007.
- Bachelor of pharmaceutical sciences (Excellent with honor), Faculty of Pharmacy, Cairo University, Beni-Suef Branch, May 2001.
Teaching experience:
Demonstrator of Analytical Chemistry (March 2003 –December 2006), associate lecturer of Analytical Chemistry (January 2007-December 2009), Assistant Professor of Analytical Chemistry (January 2010 – march 2015) and finally Associate Professor of Pharmaceutical Analytical Chemistry (April 2015 till now). Teaching for both undergraduate and postgraduate students, supervising several master and PhD students, and working as academic advisor as well.
Scientific courses and lectures of Specialty:
- General chemistry
- Qualitative analysis (analysis of cations and anions)
- Volumetric analysis (acid-base titrimetry, precipitmetry, complexometry, redox analysis)
- Instrumental analysis (principles and applications of different instrumental techniques in pharmaceutical analysis e.g. Spectroscopic methods (spectrophotometry, Spectrofluorimetry, Atomic absorption…etc), chromatographic methods (HPLC, GC, TLC…etc), electrochemical methods (conductometry, potentiometry…etc)).
- Chemometric courses (Experimental design, multivariate calibration, pattern recognition and Multivariate statistical process control for pharmaceutical industry)
- Quality control and quality assurance in pharmaceutical industry.
Research Interest:
Pharmaceutical Analytical Chemistry:
- Chromatographic methods (including HPLC and HPTLC),
- Spectrophotometric methods and spectrofluorimetric methods
- Specialist in Chemometrics and Data Analysis (2 years experience 2007-2009 in the Center for Chemometrics with professor Richard Brereton (r.g.brereton@bristol.ac.uk), University of Bristol, UK, through a joint scholarship scheme funded by the Egyptian govt.).
- The main research applications are focused on analysis of APIs in raw materials, pharmaceutical products and biological fluids, whether in mixtures or in presence of impurities and degradation products.
Publications in analytical chemistry:
- B.H. Anwar, N.S. Abdelhamid, M.A. Magdy and I.A. Naguib, Linear Support Vector Regression and Partial Least-Squares for Determination of Dapoxetine Hydrochloride and Tadalafil in Binary Pharmaceutical Mixtures, Journal of AOAC International, DOI:10.5740/jaoacint.19-0069, 2019
- B.H. Anwar, N.S. Abdelhamid, M.A. Magdy and I.A. Naguib, A Comparative Chemometric Study for Quantitative Determination of Duloxetine Hydrochloride in presence of its Toxic Impurity 1-Naphthol, Current Pharmaceutical Analysis, DOI : 10.2174/1573412915666190709093612, 2019
- F.F. Abdallah, E.A. Abdelaleem, A.A. Emam and I.A. Naguib , Determination of Pyridostigmine Bromide in presence of its related Impurities by Four Modified Classical Least Square Based Models: A Comparative Study, Current Pharmaceutical Analysis, DOI : 10.2174/1573412915666190715094347, 2019
- I.A. Naguib , E.A. Abdelaleem , A.A. Emam and F.F. Abdallah, Green Simultaneous Chromatographic Separation of Pyridostigmine Bromide and Its Related Substances in Pure Form, Tablets and Spiked Human Plasma., Journal of Chromatographic Science, doi: 10.1093/chromsci/bmz043, 2019
- I.A. Naguib, N. Abo Elyazid, F.A. Elroby and M.R. Elghobashy, Stability indicating spectrophotometric methods for quantitative determination of carbamazepine and its degradation product, iminostilbene, in pure form and pharmaceutical formulations, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 214, 21-31, 2019, DOI: 10.1016/j.saa.2019.01.080
- E.A. Abdelaleem, A.A. Emam, I.A. Naguib, F.F. Abdallah, Novel Manipulations of Ratio Spectra as Powerful Tools for Resolution and Quantitative Determination of Pyridostigmine Bromide and Its' Related Substances; A comparative study, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, https://doi.org/10.1016/j.saa.2018.11.011, 210, 66-75, 2019
- I.A. Naguib, E.A. Abdelaleem, H.E. Zaazaa, E.A. Hussein and I. Alsalahat , Development and validation of spectrophotometric methods for the determination of amoxicillin trihydrate and dicloxacillin sodium in their binary mixture, Analytical Chemistry Letters, 8(6), 844-861, 2018
- I.A. Naguib, E.A. Abdelaleem, E.S. Hassan & N.W. Ali, HPTLC method for Simultaneous Determination of Norfloxacin and Tinidazole in presence of Tinidazole Impurity, Journal of Chromatographic Science, doi: 10.1093/chromsci/bmy085, 57(1), 81-86, 2018
- A.S. Saad, I.A. Naguib, M.E. Draz, H.E. Zaazaa and A.S. Lashien, Studying the Effect of Membrane Thickness on the Performance of Green ISE-Potentiometric Sensors: Application to Ritodrine HCl and Its Active Impurity, Tyramine, Journal of The Electrochemical Society, 165(11): H764-H769, 2018
10. H.W. Darwish, I.A. Naguib, I.A. Darwish, Five modified classical least squares based models for stability indicating analysis of cyclobenzaprine HCl with its major degradation products: A comparative study, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2018, https://doi.org/10.1016/j.saa.2018.06.101, 204, 598-602, 2018
11. E.A. Abdelaleem, I.A. Naguib, S.A. Farag, and H.E. Zaazaa, Reversed phase high performance liquid chromatography and high performance thin layer liquid chromatography methods for simultaneous determination of Theophylline, Guaifenesin and Guaifenesin impurity (Guaiacol) in their bulk powders and in dosage form, Journal of Chromatographic Science, 56(9), 846-852, 2018, doi: 10.1093/chromsci/bmy062
12. I.A. Naguib, E.A. Abdelaleem, A.A. Emam, N.W. Ali and F.F. Abdallah, Development and validation of HPTLC and Green HPLC methods for Determination of Furosemide, Spironolactone and Canrenone, in Pure forms, Tablets and Spiked Human Plasma, Biomedical Chromatography, 32(10), e4304, 2018, DOI: 10.1002/bmc.4304
13. N.A. Ali, M.M. Abdelrahman, I.A. Naguib and M.R. El Ghobashy, Stability indicating HPLC and HPTLC methods for determination of agomelatine and its degradation products, Journal of Chromatographic Science, doi: 10.1093/chromsci/bmx114, 56(4), 317-326, 2018
14. M.M Abdelrahman, I.A Naguib, M.A. Elsayed, H.A Zaazaa, Chromatographic Methods for Quantitative Determination of Ampicillin, Dicloxacillin and Their Impurity 6-Aminopenicillanic Acid, Journal of Chromatographic Science, https://doi.org/10.1093/chromsci/bmx101, 56(3), 209-215, 2018
15. A.A. Emam, E.A. Abdelaleem, I.A. Naguib, F.F. Abdallah, N. Ali, Successive ratio subtraction as a novel manipulation of ratio spectra for quantitative determination of a mixture of furosemide, spironolactone and canrenone, Spectrochimica Acta Part A: Mollecular and Biomolecular Spectroscopy, 192, 427–436, 2018
16. M.M. Abdelrahman, I.A. Naguib, H.M. Nagieb, H.E. Zaazaa, Different spectrophotometric methods for determination of miconazole nitrate and hydrocortisone in bulk and in topical pharmaceutical preparation without prior separation, Chemistry Research Journal, 2(2), 56-65, 2017
17. I.A. Naguib, Improved predictions of nonlinear support vector regression and artificial neural network models via preprocessing of data with orthogonal projection to latent structures: A case study, Bulletin of Faculty of Pharmacy, Cairo University, 55. 287–291, 2017
18. H.Fahim, W.El Rouby, A. El-Gendy, A.Khairalla, I.A. Naguib, and A. Farghali, Enhancement of the productivity of the potent bacteriocin avicin A and improvement of its stability using nanotechnology approaches, Scientific Reports, DOI: 10.1038/s41598-017-10157-9, 7(1):10604, 2017
19. A.S. Saad, I.A. Naguib, M.E. Draz, H.E. Zaazaa, A.S. Lashin, Validated Analytical Methods for the Determination of Drugs Used in the Treatment of Hyperemesis Gravidarum in Multiple Formulations, Journal of AOAC International,. 101(2), 427-436, 2017
20. I.A. Naguib, E.A. Abdelaleem, S.A. Farag & H.E. Zaazaa, Simultaneous determination of Guaifenesin, Salbutamol Sulfate or Dextromethorphan HBr and Guaifenesin impurity (Guaiacol) by HPTLC Method, Analytical Chemistry Letters, http://dx.doi.org/10.1080/22297928.2017.1325778, 7(2),142-152, 2017
21. M.M. Elkhoudary, I.A. Naguib, R.A. Abdel Salam and G.M. Hadad, Comparison between two linear supervised learning machines' methods with principle component based methods for the spectrofluorimetric determination of Agomelatine and its degradants, Journal of Fluorescence, DOI 10.1007/s10895-017-2050-1, 27(3), 1149-1160, 2017
22. M.E. Abou Kull and I.A. Naguib, Simultaneous Determination of Hydrochlorothiazide and its Impurities (Chlorothiazide and Salamide) in a Quaternary Mixture with Candesartan Cilexetil by HPTLC Method, Current Pharmaceutical Analysis, 13(2), 188-194, DOI: 10.2174/1573412911666151020003509, 2017
23. N.F. Farid, I.A. Naguib, R.S. Moatamed,and M.R. El-Ghobashy, TLC-densitometric and RP-HPLC methods for simultaneous determination of Dexamethasone and Chlorpheniramine maleate in presence of methyl and propyl paraben, Journal of AOAC International, 100(1), 51-58, 2017
24. M.M. Abdelrahman, I.A. Naguib, M.A. Elsayed and H.A. Zaazaa, Spectrophotometric Methods for Quantitative Determination of Chlorhexidine Gluconate and its Major Impurity, Metabolite and Degradation Product: Para-chloro-aniline, Analytical Chemistry letters, 6(3), 232-248, 2016
25. N.F. Farid, I.A. Naguib, R.S. Moatamed,and M.R. El-Ghobashy, Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity, European Journal of Chemistry, 7 (2), 201-205, 2016
26. I.A. Naguib, E.A. Abdelaleem, H.E. Zaazaa and E.A. Hussein, Partial Least Squares and Linear Support Vector Regression Chemometric models for Simultaneous Determination of Amoxicillin Trihydrate and Dicloxacillin Sodium in Presence of Their Common Impurity, Journal of AOAC international, 99(4), 1-8, 2016.
27. I.A. Naguib and H.W. Darwish, Determination of cefoperazone sodium in presence of related impurities by improved classical least squares chemometric methods: a comparative study, Journal of Chemistry, Volume 2016, Article ID 7570643, 8 pages, 2016.
28. N.A. Ali, M.M. Abdelrahman, I.A. Naguib and M.R. El Ghobashy, Least-Squares Regression and Spectral Residual Augmented Classical Least-Squares Chemometric Models for Stability-Indicating Analysis of Agomelatine and Its Degradation Products: A Comparative Study, Journal of AOAC International, DOI: http://dx.doi.org/10.5740/jaoacint.15-0286, 99(2), 386-395, 2016
29. E.A. Abdelaleem, I.A. Naguib, E.S. Hassan & N.W. Ali, Development and Validation of Three Spectrophotometric Methods for Simultaneous Determination of Paracetamol and Pamabrom in Bulk and Pharmaceutical Formulation, Analytical Chemistry Letters, DOI: http://dx.doi.org/10.1080/22297928.2016.1138882, 6 (1), 13 – 23, 2016
30. I.A. Naguib, E A. Abdelaleem, F.F. Abdallah & N.W. Ali, Development and Validation of Three Spectrophotometric Methods for Determination of Cyclobenzaprine Hcl in The Presence of its Two Major Degradation Products, Analytical Chemistry Letters, DOI: http://dx.doi.org/10.1080/22297928.2016.1153432, 6 (1), 24 – 34, 2016
31. E.A. Abdelaleem, I.A. Naguib, H.E. Zaazaa, and E.A. Hussein, Development and Validation of HPLC and HPTLC Methods for Determination of Cefoperazone and Its Related Impurities, Journal of Chromatographic Science, doi: 10.1093/chromsci/bmv125, 54(2):179-86. 2016
32. H.W. Darwish, A.H. Bakheit, and I.A. Naguib, Comparative Study of Novel Ratio Spectra and Isoabsorptive Point Based Spectrophotometric Methods: Application on a Binary Mixture of Ascorbic Acid and Rutin, Journal of Analytical Methods in Chemistry, Article ID 2828647, 12 pages, http://dx.doi.org/10.1155/2016/2828647, Volume 2016
33. H.W. Darwish, I.A. Naguib and A.H. Bakheit. Stability Indicating Spectrofluorimetric Analysis of Metopimazine by Signal Enhanced - Partial Least Squares Chemometric Models: a Comparative Study, Current Pharmaceutical Analysis, DOI: 10.2174/1573412912666151207185931, 12(3), 234-243, 2015
34. M.M. Abdelrahman, I.A. Naguib, M.A. Elsayed and H.A. Zaazaa, Three Spectrophotometric Methods for Simultaneous Determination of Ampicillin and Dicloxacillin in Presence of Their Major Impurity 6-Aminopenicillanic Acid, Austin Journal of Analytical and Pharmaceutical Chemistry, 2(5): 1050, 2015
35. I.A. Naguib, E.A. Abdelaleem, H.E. Zaazaa, and E.A. Hussein, Determination of Cefoperazone Sodium in Presence of Related Impurities by Linear Support Vector Regression and Partial Least Squares Chemometric Models, Journal of Analytical Methods in Chemistry, , Article ID 593892, 8 pages, http://dx.doi.org/10.1155 /2015/593892, Volume 2015
36. E.A. Abdelaleem , I.A. Naguib, F.F. Abdallah and N.W. Ali, Development and validation of three spectrophotometric methods for determination of pyridostigmine bromide in the presence of its alkaline-induced degradation product, European Journal of Chemistry, 6 (3), 350-356, 2015
37. I.A. Naguib, E.A. Abdelaleem, M.E. Draz and H.E. Zaazaa, Development and Validation of RP-HPLC Method for Determination of Hydrochlorothiazide, Amiloride Hydrochloride and Related Impurities in Bulk and Pharmaceutical Dosage Forms, Analytical Chemistry Letters, 5 (2), 85 – 93, 2015
38. N.W. Ali, E.A. Abdelaleem, I.A. Naguib, and F.F. Abdallah, Development and Validation of a Stability-Indicating High-Performance Thin-Layer Chromatographic Method for Determination of Pyridostigmine Bromide in the Presence of Its Alkaline-Induced Degradation Product, Journal of Planar Chromatography, 28 (4), 316–322, 2015
39. E.A. Abdelaleem, I.A. Naguib, E.S. Hassan and N.W. Ali, HPTLC and RP-HPLC methods for simultaneous determination of Paracetamol and Pamabrom in presence of their potential impurities, Journal of Pharmaceutical and Biomedical Analysis, 114, 22-27, 2015
40. N.A. Ali, M.M. Abdelrahman, I.A. Naguib and M.R. El Ghobashy, Development of Membrane Electrode for the Selective determination of Bromazepam in Tablets and Plasma, Analytical and Bioanalytical Electrochemistry, 7, 242-253, 2015
41. I.A. Naguib, M. M. Abdelrahman, M. R. El Ghobashy and N. A. Ali, HPTLC Method for Quantitative Determination of Zopiclone and Its Impurity, Journal of Chromatographic Science,1–5, doi:10.1093/chromsci/bmv015, 53(8), 1395-1399, 2015
42. M. M. Abdelrahman, I. A. Naguib, M. R. El Ghobashy and N. A. Ali, Quantitative determination of Zopiclone and its impurity by four different Spectrophotometric methods, Spectrochimica Acta Part A: Mollecular and Biomolecular Spectroscopy, 137, 617-624, 2015
43. I.A. Naguib, E. A. Abdelaleem, M. E. Draz, H. E. Zaazaa, Linear support vector regression and partial least squares chemometric models for determination of Hydrochlorothiazide and Benazepril hydrochloride in presence of related impurities: A comparative study, Spectrochimica Acta Part A: Mollecular and Biomolecular Spectroscopy, 130, 350–356, 2014
44. E. A. Abdelaleem, I. A. Naguib, H. E. Zaazaa and M. E. Draz, Spectrophotometric Methods for Quantitative Determination of Binary Mixture of Hydrochlorothiazide and Amiloride Hydrochloride without Prior Separation, Asian J. of Biomed. & Pharm. Sci., 4 (34); 27-33, 2014
45. I. A. Naguib, M. M. Abdelrahman, Stability indicating HPTLC method for determination of Metopimazine in pharmaceutical formulation and human plasma, Beni-Suef University Journal of Basic and Applied Sciences, 3 (1), 52–62, 2014
46. I. A. Naguib, E. A. Abdelaleem, H. E. Zaazaa and M. E. Draz, Simultaneous Determination of Hydrochlorothiazide and Benazepril Hydrochloride or Amiloride Hydrochloride in Presence of Hydrochlorothiazide Impurities: Chlorothiazide and Salamide by HPTLC Method, Journal of Chromatographic Science, doi:10.1093/chromsci/bmu016, 53(1), 183-188, 2015
47. I.A. Naguib, E.A. Abdelaleem, H.E. Zaazaa and E.A. Hussein, "Development and validation of stability indicating spectrophotometric and HPTLC methods for determination of acemetacin", European Journal of Chemistry, 5 (2), 219-226, 2014
48. E.A. AbdelAleem, I.A. Naguib, H.E. Zaazaa, M.E. Draz, “Simultaneous determination of some antihypertension drugs in their binary mixtures by simple spectrophotometric methods”, Asian Journal. of Biomededical & Pharmaceutical Sciences, 03 (25); 5-12, 2013.
49. I. A. Naguib and H.W. Darwish, Support vector regression and artificial neural network models for stability indicating analysis of mebeverine hydrochloride and sulpiride mixtures in pharmaceutical preparation: A comparative study, Spectrochimica Acta Part A: Mollecular and Biomolecular Spectroscopy, 86, 515-526, 2012.
50. H.W. Darwish and I.A. Naguib, Improved partial least squares models for stability-indicating analysis of mebeverine and sulpiride mixtures in pharmaceutical preparation: A comparative study, Drug Testing and Analysis, 5(5), 325-333, 2011.
51. I.A. Naguib, Stability indicating analysis of bisacodyl by partial least squares regression, spectral residual augmented classical least squares and support vector regression chemometric models: A comparative study, Bulletin of Faculty of Pharmacy Cairo University.,49, 91-100, 2011.
52. I.A. Naguib and M. Abdelkawy, Development and validation of stability indicating HPLC and HPTLC methods for determination of sulpiride and mebeverine hydrochloride in combination, European Journal of Medicinal Chemistry.,45, 3719-3725, 2010.
53. F. H. Metwally, M. Abdelkawy, I.A. Naguib, Development and validation of three stability-indicating methods for determination of bisacodyl in pure form and Pharmaceutical Preparations, Journal of AOAC International, 90, 113-127, 2007.
54. F. H. Metwally, M. Abdelkawy, I.A. Naguib, Determination of nifuroxazide and drotaverine hydrochloride in pharmaceutical preparations by three independent analytical methods, Journal of AOAC International, 89, 78-87, 2006.
55. F. H. Metwally, M. Abdelkawy, I.A. Naguib, Development and validation of three stability indicating analytical methods for determination of metopimazine in pharmaceutical preparation, Bulletin of Faculty of Pharmacy Cairo University.,44, 1-15, 2006.
Conferences:
- International Conference on Chromatographic Data Visualization (2008, Winchester, UK).
- 1st International conference of Advanced Basic and Applied Sciences (ABAS, 2012, Hurghada, Egypt) N.B. Best oral presentation reward and organizer http://www.uv.edu.eg/en/bsu/item/22626-the-1st-international-conference-on-advanced-basic-applied-sciences-abas)
- 4th FUE international conference of pharmaceutical sciences (4th FUE ICPS), 31 Jan – 2 Feb 2017 (New cairo, Dusit Thani hotel, Egypt), Poster presentation: Improved predictions of nonlinear support vector regression and artificial neural network models via preprocessing of data with orthogonal projection to latent structures: A case study.
- First International Conference of Pharmaceutical and Medical Sciences of Suez Canal University 1-2 April 2017 (Cairo, Egypt), Poster presentation: Development and validation of Liquid Chromatography/ Mass Spectrometric method for simultaneous determination of Amoxicillin and Dicloxacillin in binary mixture in presence of their common impurity.
- 2nd Ain Shams University international conference for pharmaceutical sciences ICPASU November 13th-15th, 2018, Novotel Cairo Airport, poster presentation: Development and validation of Liquid Chromatography/ Mass Spectrometric method for simultaneous determination of Amoxicillin and Dicloxacillin in binary mixture in presence of their common impurity.
- 5th FUE international conference of pharmaceutical sciences (5th FUE ICPS), 28-30 Jan 2019 (Royal maxime Kempeniski hotel), invited speaker: Cheomometrics and pharmaceutical analysis (29th)
Research funds
1- Utility of HPTLC as analytical method for analysis of Candesartan cilexetil and hydrochlorothiazide in presence of its impurities, Tabuk University, deanship of scientific research, project ID: 0096-1436-s. (Co-PI)
2- Development of advanced analytical chemometric methods for analysis of cefoperazone in presence of its impurities Tabuk University, deanship of scientific research, project ID: 0054-1436-s. (PI)
General certificates:
- Toefl (iBT score 103/120).
- International Computer driving License (ICDL).
- Certified Trainer and Human Resources developer (CT & HRD) certified by IBCT http://www.ibct-global.com/Directory.aspx ) 2011.
- Certified Entrepreneurship trainer by ILO 2011.
- UNILEAD training program certificate (DAAD, Germany) 2013-2014.
- Program and course specifications design (NAQAAE) 2017.
Main managerial responsibilities:
- Director of computer center in faculty of pharmacy, Beni-Suef University 2010.
- Director of International Relations Office (IRO) at Beni-Suef University (Oct 2010 – Feb 2012).
- Director of Faculty and Leadership FLDC training centre in Beni-Suef University (Jan 2012 till Oct 2013 http://www.bsu.edu.eg/TeachingStaff.aspx).
- General coordinator of “Pathways to higher education” student’s training project in Beni-Suef University (Jan 2012 till Oct 2013 http://www.bsu.edu.eg/New_Details.aspx?Id=4729).
- Deputy Dean of academic affairs in faculty of pharmacy, Tabuk University (UT), Tabuk, Kingdom of Saudi Arabia (2014-2015).
Membership:
- Member in the Egyptian General Syndicate of Pharmacists
- Steering committee member in Beni-Suef Syndicate of Pharmacists, Beni-Suef, Egypt (2011-2013).
- Internationally Certified Trainer & HRD in the NCFLD (Egypt) and IBCT (Netherlands).
Honorary:
- Silver Medal – General Syndicate of Pharmacists, Cairo, Egypt.
- Who's Who honored scientist in Medicine and Healthcare 2011-2012 (8th Edition).
Professional career history:
- Training in private pharmacies in Egypt for 3 years (1997-2000)
- Training in a pharmaceutical company in Egypt for 10 days (EIPICO pharmaceuticals: quality control, quality assurance, manufacturing), 1999
- Bachelor in pharmaceutical sciences (Cairo university, Beni-Suef Branch, 2001)
- Working in private pharmacies in Egypt for 6 months (2001)
- Master in pharmaceutical analytical chemistry (Cairo university, 2006)
- PhD in pharmaceutical analytical chemistry (Beni-Suef University, 2009). Through my 3 years PhD work, I joined Professor Richard Brereton’s center for chemometrics for 2 years (University of Bristol, UK).
- Working partly for 5 months with GSK pharmaceutical company in UK in joint collaboration with center for chemometrics (University of Bristol) during my PhD studies. The research point was related to online monitoring of drug impurities during manufacturing of one of the active pharmaceutical ingredients. The results are presented in one of the PhD thesis chapters.
- Coordinator of advanced instrumental analysis diploma in the faculty of post-graduate studies, Beni-Suef University.
- Supervisor of stability and bioequivalence studies' unit in the faculty of post-graduate studies, Beni-Suef University.
- Working as pharmaceutical product registration consultant for two toll pharmaceutical manufacturing companies in Egypt (International Pharma Group Co. IPG, and Egyptian-Canadian for Drugs Co.)
- Certified Trainer and Human Resources developer (CT & HRD) certified by International Board of Certified Trainers IBCT http://www.ibct-global.com/Directory.aspx) with several experiences in TOT training programs and HRD project management allover Egypt (Mansoura, Zagazig, Aexandria, Kafrelsheikh, Ismailia, Cairo and Beni-Suef).
- Certified local Entrepreneurship trainer by International Labor Organization ILO.
- DAAD Alumni: granted scholarship in Germany for University leaders (UNILEAD training project; which comprises project management module, financial resources module and human resources management module), 2013-2014 http://www.scholarshipstimes.com/2013/03/20/2013-2014-unilead-programme-at-university-of-oldenburg-germany/
- Several activities as a trainer and HRD consultant for NCFLD and my University for 3 years in the following programs:
- TOT
- Credit hours system program
- International publication
- How to compete for a research fund
- Code of Ethics
- Strategic planning.
- Negotiation skills.
- Communication skills
- Presentation skills
- Analytical thinking
- Entrepreneurship
- Time and meeting management
- Effective University Management
- Capability to plan and manage HRD training projects. I designed the “Research moderators” training project for my University in Egypt.
- Several societal activities through my work as a board member in the syndicate of pharmacists in my governorate (Beni-Suef), where we could plan and offer continuous learning programs for pharmacists, societal awareness campaigns against hepatitis C virus and others.
- Assistant professor in pharmaceutical chemistry department in faculty of pharmacy, University of Tabuk (UT), Tabuk, Kingdom of Saudi Arabia (2014-2016).
- Associate professor of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Beni-Suef University, April 2015 – Jan 2019.
- Supervisor of quality assurance committees in faculty of pharmacy, Beni-Suef Unievrsity for quality management and for academic programs and standards 2017-2018
- Departmental seminar coordinator for pharmaceutical analytical chemistry department 2018.
Master Title
Analytical Study on Some Drugs used for Treatment of the Gastrointestinal Tract
Master Abstract
Master Thesis Abstract
By: Ibrahim Ahmed Naguib Yassen
'' Analytical Study on Some Drugs used for Treatment of the Gastrointestinal Tract ''
The thesis comprises four parts:
Part I: General Introduction
This part comprises a brief idea about gastrointestinal disorders and GIT drugs' classification and mechanism of action.
Part II: Simultaneous Determination of Nifuroxazide and Drotaverine hydrochloride in Pure Forms and in Pharmaceutical Preparation
This part includes a general introduction about the chemistry and mode of action of nifuroxazide and drotaverine hydrochloride, followed by a presentation of the reported methods used for their quantitative determination. Experimental, results and discussion are also given.
This part comprises three sections:
Section (A): Simultaneous Determination of Nifuroxazide and Drotaverine hydrochloride by the Spectrophotometric Method
In this section, nifuroxazide could be determined in presence of drotaverine hydrochloride using a zero order spectrum with an analytically useful maximum at 364.5 nm. The absorbance obeyed Beer's law over a concentration range 2 – 10 µg.ml-1 with mean percentage recovery 100.08 ± 0.606 %. Determination of drotaverine hydrochloride in presence of nifuroxazide was obtained by second derivative D2 spectrophotometry at 243.6 nm. The peak height response obeyed Beer's law over a concentration range of 2 – 10 µg.ml-1 with mean percentage recovery 99.82 ± 1.461 %.
The selectivity of the proposed method was checked using laboratory prepared mixtures and it was successfully applied to the analysis of the pharmaceutical formulation containing the above drugs with no interference from other dosage form additives.
The validity of the suggested procedure was further assessed by applying the standard addition technique.
The results of the proposed method were statistically compared with the reference method adopted by the manufacturer company. The t and F values were found to be less than the tabulated ones indicating no significant difference with respect to accuracy and precision.
Section (B): Simultaneous Determination of Nifuroxazide and Drotaverine hydrochloride by the Spectrodensitometric Method
In this section, both drugs are separated on a silica gel plate using chloroform: acetone: methanol: glacial acetic acid (6: 3: 0.9: 0.1 v/v/v/v) as a mobile phase and UV detection of both bands at 365 nm over a concentration range of 0.2 – 1 µg.band-1 for both drugs, with mean percentage recoveries 99.99 ± 0.148 % and 100.00 ± 0.335 % for nifuroxazide and drotaverine hydrochloride respectively.
The selectivity of the proposed method was checked using laboratory prepared mixtures. It was successfully applied to the analysis of the pharmaceutical formulation containing the above drugs with no interference from other dosage form additives.
The validity of the suggested procedure was further assessed by applying the standard addition technique.
The results of the proposed method were statistically compared with the reference method adopted by the manufacturer company. The t and F values were found to be less than the tabulated figures indicating no significant difference with respect to accuracy and precision.
Section (C): Simultaneous Determination of Nifuroxazide and Drotaverine hydrochloride by the RP-HPLC Method
In this section, a RP-HPLC method was applied which utilizes acetonitrile: water (40/60 v/v, adjusted to pH 2.55 by orthophosphoric acid) as a mobile phase and pentoxifylline as internal standard. The flow rate is 1 ml.min-1 and the effluent is detected at 285 nm at ambient temperature over a concentration range 2 – 10 µg.ml-1 for both drugs with mean percentage recoveries 100.24 ± 1.511 % and 100.08 ± 0.778 % for nifuroxazide and drotaverine hydrochloride respectively.
The selectivity of the proposed method was checked using laboratory prepared mixtures and it was successfully applied to the analysis of the pharmaceutical formulation containing the above drugs with no interference from other dosage form additives.
The validity of the suggested procedure was further assessed by applying the standard addition technique.
The results of the proposed method were statistically compared with the reference method adopted by the manufacturer company. The t and F values were found to be less than the tabulated figures indicating no significant difference with respect to accuracy and precision.
Part III: Determination of Bisacodyl in Pure Form and Pharmaceutical Preparations by Three Stability Indicating Methods
This part includes a general introduction about the chemistry and mode of action of bisacodyl, followed by a presentation of the reported methods used for its quantitative determination. Experimental, results and discussion were also given.
This part comprises three sections:
Section (A): Spectrodensitometric Determination of Bisacodyl in Presence of its Degradation Products
In this section, bisacodyl was determined via separation from its degradation products on silica gel plates using chloroform: acetone (9: 1 v/v) as a mobile phase with UV detection of the separated bands at 223 nm over a concentration range of 0.2 – 1.4 µg.band-1 with mean percentage recovery 100.35 ± 1.923 %.
The selectivity of the proposed method was checked using laboratory prepared mixtures and it was successfully applied to the analysis of the pharmaceutical formulations containing bisacodyl with no interference from other dosage form additives.
The validity of the suggested procedure was further assessed by applying the standard addition technique.
The results of the proposed method were statistically compared with the HPLC reference method. The t and F values were found to be less than the tabulated figures indicating no significant difference with respect to accuracy and precision.
Section (B): Spectrophotometric Determination of Bisacodyl in Presence of its Degradation Products
In this section, a fourth derivative D4 spectrophotometric method was applied, which allows determination of bisacodyl in presence of its degradation products in raw material at 223 nm using acetonitrile as solvent with obedience to Beer's law over a concentration range 2 – 18 µg.ml-1 with mean percentage recovery 99.77 ± 1.056 %.
The selectivity of the proposed method was checked using laboratory prepared mixtures.
The results obtained for the analysis of pure samples of bisacodyl were statistically compared with those obtained by applying the HPLC reference method and no significant difference between the results was obtained.
Section (C): Determination of Bisacodyl in presence of its Degradation Products by Three Spectrophotometric Multivariate Methods
In this section, the spectrophotometric data of bisacodyl and its degradation products using absolute ethanol as solvent were processed by three chemometric techniques namely classical least squares (CLS), principal component regression (PCR) and partial least squares (PLS). A training set consisting of 15 mixtures containing different ratios of Bisacodyl and its degradation products was used for construction of the three models. A validation set consisting of 6 mixtures was used to validate the prediction ability of the suggested models. The three mentioned chemometric methods were applicable over a concentration range between 2 - 14 µg.ml-1 with mean percentage recovery 99.97 ± 0.865, 100.01 ± 0.749 and 99.97 ± 0.616 for the three mentioned models respectively.
The selectivity of the proposed method was checked using laboratory prepared mixtures (validation set) and it was successfully applied to the analysis of the pharmaceutical formulation containing bisacodyl with no interference from other dosage form additives.
The validity of the suggested procedure was further assessed by applying the standard addition technique.
The results of the proposed method were statistically compared with the HPLC reference method. The t and F values were found to be less than the tabulated figures indicating no significant difference with respect to accuracy and precision.
Part IV: Stability Indicating Methods for Determination of Metopimazine in Presence of its Oxidative Degradation Product
This part includes a general introduction about the chemistry and mode of action of metopimazine, followed by a presentation of the reported methods used for its quantitative determination. Experimental, results and discussion were also given.
This part comprises three sections:
Section (A): Spectrofluorimetric Determination of Metopimazine in Presence of its Oxidative Degradation Product.
In this section, metopimazine was determined by measuring its native fluorescence in presence of its oxidative degradate at ?em 505 nm upon excitation with ?ex 336 nm after applying in a finally processed equation over a concentration range 0.1 - 2 µg.ml-1 with mean percentage recovery 100.64 ± 1.349 %.
The method was further applied to the in vitro determination of metopimazine in spiked human serum. The mean % recovery (n = 4) was 98.09 ± 0.390 %.
The selectivity of the proposed method was checked using laboratory prepared mixtures and it was successfully applied to the analysis of the pharmaceutical formulation containing metopimazine with no interference from other dosage form additives.
The validity of the suggested procedure was further assessed by applying the standard addition technique.
The results of the proposed method were statistically compared with the reference method adopted by the manufacturer company. The t and F values were found to be less than the tabulated figures indicating no significant difference with respect to accuracy and precision.
Section (B): Spectrophotometric Determination of Metopimazine in Presence of its Oxidative Degradation Product.
In this section, a second derivative D2 spectrophotometric method was applied. It allows determination of metopimazine without interference of its oxidative degradate at 270.5 nm using methanol as a solvent, with obedience to Beer's law over a concentration range 1 - 16 µg.ml-1 with mean percentage recovery 100.13 ± 1.660 %.
The selectivity of the proposed method was checked using laboratory prepared mixtures and it was successfully applied to the analysis of the pharmaceutical formulation containing metopimazine with recovered interference from other dosage form additives.
The validity of the suggested procedure was further assessed by applying the standard addition technique.
The results of the proposed method were statistically compared with the reference method adopted by the manufacturer company. The t and F values were found to be less than the tabulated figures indicating no significant difference with respect to accuracy and precision.
Section (C): Spectrodensitometric Determination of Metopimazine in Presence of its Oxidative Degradation Product.
In this section, metopimazine was determined via separation from its oxidative degradate on silica gel plates using chloroform: methanol (6: 4 v/v) as a mobile phase and UV detection of the separated bands at 265 nm over a concentration range of 0.4 – 1.4 µg.band-1 with mean percentage recovery 100.18 ± 1.562 %.
The selectivity of the proposed method was checked using laboratory prepared mixtures and it was successfully applied to the analysis of the pharmaceutical formulation containing metopimazine with no interference from other dosage form additives.
The validity of the suggested procedure was further assessed by applying the standard addition technique.
The results of the proposed method were statistically compared with the reference method adopted by the manufacturer company. The t and F values were found to be less than the tabulated figures indicating no significant difference with respect to accuracy and precision.
PHD Title
Adoption of Chemometric and Instrumental Techniques for the Analysis of Drugs in Combinations
PHD Abstract
PhD Thesis Abstract
Adoption of Chemometric and Instrumental Techniques for the Analysis of Drugs in Combinations
The thesis comprises four parts:
Part 1: Introduction to Chemometrics
This part comprises a brief idea about the definition and origin of chemometrics. It also presents the different topics of chemometrics like multivariate calibration, pattern recognition, signal processing, experimental design, multiway analysis, curve resolution methods, process control and others. This introduction gives examples for applications of chemometrics in the medical and pharmaceutical fields as well.
Part 2: Development and Validation of Stability Indicating HPLC and HPTLC Methods for Determination of Sulpiride and Mebeverine Hdrochloride in Combination
This part includes a general introduction about the chemistry and mode of action of sulpiride and mebeverine hydrochloride, followed by the presentation of the reported methods used for their quantitative determination. Experimental, results and discussion are also given.
This part comprises two sections:
Section A: A Stability Study for the Simultaneous Determination of Sulpiride and Mebeverine Hydrochloride by HPLC Hethod
In this section, mixtures of the sulpiride and mebeverine hydrochloride together with the reported interferants are separated on a reversed phase cyano column (5µm ps, 250mm x 4.6 id) using acetonitrile:water (70:30 v/v) adjusted to pH=7 as a mobile phase and metopimazine (MPZ) is used as internal standard. The drugs were detected at 221 nm over a concentration range of 5 – 40 µg.ml-1 for sulpiride with mean percentage recovery = 99.75 ± 0.910 % and 5 – 60 µg.ml-1 for mebeverine hydrochloride with mean percentage recovery = 99.99 ± 0.450 %.
The selectivity of the proposed method was checked using laboratory prepared mixtures. The method was successfully applied to the analysis of the pharmaceutical formulation containing sulpiride and mebeverine hydrochloride with no interference from other dosage form additives. The percentage recoveries obtained were in accordance with those given by the reference method.
The validity and accuracy of the suggested procedure were further assessed by applying the standard addition technique.
The results of the proposed method were statistically compared with the reference HPLC method reported in the literature. The t and F values were found less than the tabulated figures indicating no significant difference with respect to accuracy and precision.
Section B: A Stability Study for the Simultaneous Determination of Sulpiride and Mebeverine Hydrochloride by HPTLC Method
In this section, both drugs are separated on an HPTLC plate coated with silica gel 60 F254 using absolute ethanol: methylene chloride: triethyl amine (7: 3: 0.2 by volume) as a mobile phase and scanning of the separated bands at 221 nm over a concentration range of 0.4 – 1.4 µg.band-1 for sulpiride with mean percentage recovery 101.01 ± 1.991 % and 0.2 – 1.6 µg.band-1 for mebeverine hydrochloride with mean percentage recovery 100.40 ± 1.868 %.
The selectivity of the proposed method was checked using laboratory prepared mixtures. The method was successfully applied to the analysis of the pharmaceutical formulation containing sulpiride and mebeverine hydrochloride with no interference from other dosage form additives. The percentage recoveries obtained were in accordance with those given by the reference method.
The validity and accuracy of the suggested procedure were further assessed by applying the standard addition technique.
The results of the proposed method were statistically compared with the reference HPLC method reported in the literature. The t and F values were found less than the tabulated figures indicating no significant difference with respect to accuracy and precision.
Part 3: Partial Least Squares Regression, Spectral Residual Augmented Classical Least Squares and Support Vector Regression: A Comparative Study for Analysis of Colona® Tablets
Partial least squares regression (PLSR), spectral residual augmented classical least squares (SRACLS) and support vector regression (SVR) are amongst the best multivariate regression methods used recently for pharmaceutical analysis, although each of them has its unique performance.
The work introduced in this part aims to compare these three different chemometric methods, showing the underlying algorithm for each and making a modest comparison amongst them to indicate the merits and demerits of each. It also helps to highlight the importance of considering robustness and ruggedness of chemometric techniques for real life routine analysis, where recalibration is not usually easy to perform, while robustifiability of certain models could make it supersede other advanced models from the point of view of industry.
To project the comparison in a sensible way, the three methods are used for the stability indicating quantitative analysis of mixtures of mebeverine hydrochloride and sulpiride in binary mixtures and in presence of their reported impurities and degradation products (summing up to 6 components) whether in raw materials or in pharmaceutical tablets via handling the UV spectral data. All the mixtures were recorded at 226-320 nm.
For proper analysis, a 6 factor 5 level experimental design was established resulting in a training set of 25 mixtures containing different ratios of the interfering species. A test set consisting of 5 mixtures was used to validate the prediction ability of the suggested models. The proposed methods were successfully applied to the analysis of pharmaceutical tablets containing mebeverine hydrochloride and sulpiride mixtures.
The methods indicate the ability of the mentioned multivariate calibration models to deconvolute the highly overlapped UV spectra of the 6 components’ mixtures and to show prediction ability that is equivalent to the hyphenated precise separation techniques like HPLC, yet using cheap and easy to handle instruments like the UV spectrophotometer. This opens the scope for easier analysis of pharmaceutical products with higher numbers of interfering components (4 and more) without the need for experience, long time and expensive complicated instruments as the case with HPLC.
The results of the proposed methods were statistically compared with the reference HPLC method reported in the literature. The t and F values were found to be less than the tabulated figures indicating no significant difference with respect to accuracy and precision (except for the SRACLS method).
Part 4: On-line HPLC for Process Monitoring: Exploratory Analysis of Campaign 1, Process GW’553, CDI Stage
GlaxoSmithKline (GSK) is an international leading company in production of pharmaceutically active ingredients and pharmaceutical dosage forms.
The data handled in this chapter are obtained through collaboration between GSK (Gunnels Wood Road, Stevenage, Hertfordshire SG1 2NY, UK) and the Centre for Chemometrics (University of Bristol, UK).
In this project, GSK is trying to develop methods for online monitoring of impurities during the process of production of one of the active pharmaceuticals. In the presented work we are concerned with the analysis of what is called “The CDI stage” as one of the stages of the process called ”GW’553”. 608 chromatograms were provided after 4 days of continuous process, where the aim of the analysis is to find out which of these 608 chromatograms are not conforming to the steady state criteria, besides finding out the reason behind the abnormality by comparing it to the parallel log book and process variables information reported by the process analyst. Signal processing methods like baseline correction, peak alignment, peak detection and integration, and peak matching were applied for proper handling of the chromatograms, then Principal Components Analysis (PCA) of the peak table data was performed coupled with implementing multivariate statistical process control charts (Q and D statistics) for finding out the outliers and determining whether they originate from systematic or non-systematic deviation reasons like the chromatographic changes and process variables changes respectively.
Keywords
Mebeverine hydrochloride, sulpiride, HPLC, HPTLC, stability indicating assay, chemometrics, PLSR, SRACLS, SVR, multivariate statistical process control, D and Q charts.