Basic Informations

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Contact Information

Surname: HUSSEIN

Name: Mortada Mohamed Abdel-Hamid Hussein Mohamed

Address: Fish Department, Faculty of Veterinary Medicine, Beni-Suef University,

Beni-Suef 62512, Egypt.

Office: Home Tel.: +20- 822- 331- 645

Telephone: +20- 822- 322- 066 Mobile: 20-103-781-059

Tel/Fax: +20- 822- 327- 982 E-mail: mortadahussein@hotmail.com

Personal Data

Date of birth: 4/6/1966

Place of birth: Algeria

Nationality: Egyptian

Marital status: Married and have two daughters and one son.

Educational Background

PhD: March 2002 in the Field of Veterinary Medicine (Fish Diseases), Nippon Veterinary & Life Science University (formally: Nippon Veterinary and Animal Science), JAPAN. Dissertation title “Studies on saprolegniasis in salmonids in Hokkaido.”

M.Sc.: March 1992 in the field of Veterinary Medicine (Infectious diseases, Fish), Faculty of Veterinary medicine, CAIRO University, Beni-Suef Brach, Egypt. Dissertation title “Some studies on gills affections of fresh-water fishes”

B.Sc.: May 1988 in Veterinary Medicine, Faculty of Veterinary medicine, CAIRO University, Beni-Suef Branch. Egypt. (Grade: very good, Rank: Fourth).

Current Position:

Associate Professor in the Fish Department , Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62512, EGYPT.

Administrative Positions:

· Executive Director of the web site 2009.

· Deputy Director of the Project Portal 2009-2010.

· Project Manager Portal 2011-2012.

Employment History

Employer	Position	Dates (From – to)
Faculty of Veterinary Medicine, Beni-Suef University	Associate Professor in the Fish Dep.	October, 2007-now
Faculty of Veterinary,Cairo University, Beni-Suef branch*	Lecturer. in the Fish Dep.	2002-2007
Division of Fish Diseases, Nippon Veterinary & Life Science University, Tokyo, JAPAN.	Post-Doc Researcher, Division of Fish Diseases, Nippon Veterinary & Life Science University, Tokyo, JAPAN	2004-2006
Division of Fish Diseases, Nippon Veterinary & Life Science University, Tokyo, JAPAN.	PhD student	1997-2002
Faculty of Veterinary, Cairo University, Beni-Suef branch	Ass. Lecturer. in the Fish Dep.	1992-1997
Fac. Vet. Med., Cairo Univ.Beni-Suef branch	Demonstrator of fish diseases, Dep. Of Internal Medicine &Infectious Dis.	1989-1992

* Beni Suef university has had separated from Cairo University since 2005

Membership of Professional Associations

Professional body	Level of membership	Year of award
Japan Society for the Promotion of Science (JSPS), Japan.	Member	2004
Member of the Japanese Society of Fish Pathology.	Member	1998
Member of the Japanese Mycological Society	Member	1999
Member of the European Association of Fish Pathologists.	Member	1999
Member of the Egyptian Veterinary Medical Association.	Member	2002
Member of the Egyptian Society for the Development of Fisheries Resources & Human Health	Member	2007

Attending Conferences & workshops

Satisfactory completion of self-evaluation of higher education institutions 13-12-2009 to 17-12-2009.
Satisfactory completion of Session of the external audit of institutions of higher education institutions 19-12-2009 to 23-12-2009.

The International Arab African Fish Resources Conference & Exhibition “Sustainable use and management of aquatic resources”, Cairo, Egypt, 2007.
Satisfactory completion of Training of Trainer workshop (TOT) FLDP, Assuit Uni. 2006.

6^th Symposium on Diseases in Asian Aquaculture “Aquatic animal health: facing new challenges”, Colombo, Sri Lanka, 2005.
KHV infection: Present Status and Future Prospects for Prevention, Tokyo, Japan, 2004.
11^th Scientific Congress of the Faculty of Vet. Med. Assiut Uni. 2004.
1^st Intern.Conf. Vet. Res. Div., NRC, Cairo, 2004.
12^th Scientific Congress of the Japanese Society of Antimicrobial Agents, Osaka, 2001.
11^th Conference of Research Projects of the Overseas Students, Nippon Medical School,Tokyo, JAPAN, 2000.
1^st Conference of Japanese Society of Ornamental Fish, Tokyo, Japan 2000.
The International Conference of the European Association of Fish Pathplogists (EAFP), Rhodes, Greece, 1999.

Honors

- Ph.D. Scholarship from July 1997 to March 2002 as a governmental Scholarship awarded from The Ministry of Higher Education of Egypt to study in Japan.

- Post-Doctoral Fellowship in Nippon Veterinary and Life Science University from November 2004 to November 2006 awarded from Japan Society for the Promotion of Science (JSPS), Japan.

Training

Training attended / Technical skills acquired	Place	Dates (From – to)
Molecular biology, cloning, sequence analysis, DNA vaccination, and others	Nippon Veterinary & Life Science University (formally: Nippon Veterinary and Animal Science), JAPAN.	Novmber 2004 to October 2006
Microinjection in fish embryos	Tokyo university of Marine Science and Technology	October 2005 to November 2005
Training of trainers ‘TOT’ for a research project of developing the capacity of faculty members and leaders.	Assiut University, Egypt	24-27/12/2006

Professional Activities

- Fish Diseases and Management of Aquaculture.

- Microbiological and Histopathological Techniques.

- Molecular biological and Cloning Techniques.

- Fish Microinjection and Transgenesis Techniques.

Academic Activities

A)Teaching undergraduates students from 1990 till now many courses including the following topics:

- Outline of Fish Biology.

- Bacterial, Viral, Mycotic, Parasitic, and Miscellaneous diseases of fishes.

- Strategy of Aquaculture and Fish farming techniques.

- Methods of controls and prevention of Fish diseases.

- Practical and field training through scientific trips.

- Molecular diagnostic techniques including: 

- Gel electrophoresis, DNA and RNA manipulation, PCR and Multiplex PCR, Restriction enzymes handling, Cloning in bacteria, and Protein purification

-Various techniques in Bacterial & Fungus isolation & Identification 

 Traditional techniques and advanced techniques.

- Different serological techniques including: 

 - ELISA(Enzyme Linked Immunosorbent Assay), Haemagglutination inhibition, passive 

  haemagglutination, agar gel precipitation test (AGPT), and IFA (immunofluorescent antibody technique).

B) Supervisor for postgraduate studies (Master and Ph D)

1. Master Degree:

·         Some studies on flavobacteriosis in freshwater fishes

Supervisors: Prof. Manal Adel Ahamed Essa      Head of Fish Dep., Faclty of Vet. Med.

                      Beni- suet Univeristy.

                      Dr. Mortada Mohamed A. Hussein    Associate Prof. fish diseases and

                      management,  Fish Dep., Faclty of Vet. Med., Beni- suet Univeristy.

                      Dr. Mohamed Abdel Azziz                    Associate Prof. fish diseases and

                      management, Fish Dis. Dep., Faclty of Vet. Med., Sohage University.

·         Some studies on mycotic  affections in freshwater fishes

Supervisors: Dr. Walead Hamdy Hassan      Associate Prof , microbiology Dep., Faclty

                         of Vet. Med., Beni- suet Univeristy.

                      Dr. Mortada Mohamed A. Hussein    Associate Prof. fish diseases and

                      management,  Fish Dep., Faclty of Vet. Med., Beni- suet Univeristy.

2. Ph D Degree:

Application of recent techniques for detection of some pathogenic bacteria in fresh and marine water fishes

Supervisors: Dr. Walead Hamdy Hassan      Associate Prof , microbiology Dep., Faclty

                         of Vet. Med., Beni- suet Univeristy.

                      Dr. Mortada Mohamed A. Hussein    Associate Prof. fish diseases and

                      management,  Fish Dep., Faclty of Vet. Med., Beni- suet Univeristy.

·         Some studies on enterosepticamia caused by Edwarseilla tarda  infwctions in freshwater fishes

Supervisors: Prof. Manal Adel Ahamed Essa      Head of Fish Dep., Faclty of Vet. Med.

                      Beni- suef Univeristy.

                      Dr. Mortada Mohamed A. Hussein    Associate Prof. fish diseases and

                      management,  Fish Dep., Faclty of Vet. Med., Beni- suet Univeristy.

                      Dr. Mohamed Abdel Azziz                    Associate Prof. fish diseases and

                      management, Fish Dis. Dep., Faclty of Vet. Med., Sohage University.

Current research interests

Molecular diagnostic tools in field of fish diseases and vaccine development.

Current research Activity

Deposition of gab C gene sequences for Streptococcus iniae, Streptococcus dysgalactiae, and Lactococcus garvieae in GenBank with access numbers as following:

SI GQ912305                        Streptococcus iniae gab C gene sequence Published
SD GQ912306                     Streptococcus dysgalactiae gab C gene sequence Published
LG GQ912307                     Lactococcus garvieae gab C gene sequence Published

Deposition of Aeromonas hydrophila strain Egypt/BSN-15614 cytotoxic enterotoxin gene, Aeromonas veronii strain Egypt/BSN-23514 cytotoxic enterotoxin gene, Aeromonas veronii strain Egypt/BSN-19714 cytotoxic enterotoxin gene, partial cds, GenBank with access numbers as following:

KU582045 Aeromonas hydrophila strain Egypt/BSN-15614.

KU258046 Aeromonas veronii strain Egypt/BSN-23514.

KU258047 Aeromonas veronii strain Egypt/BSN-19714.

Other Skills

Languages:

Arabic: as a mother tongue.

English: (read, speak and writing "Excellent")

Japanese: (speak "good", read and writing “fair”)

Computer: Using of several microcomputer applications and software i.e. Microsoft (Windows and Office), Statistics, internet (web design), Graphics (photoshop), and Multimedia software.

Reference:

Name	Contact address	E-mail/Tel
Prof. Dr. Kishio Hatai Laboratory of Fish Diseases, School of Veterinary Medicine,	Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan	k-hatai.0111@scan-net.ne.jp or khatai0111@nvlu.ac.jp Tel:(+81) 422-31-4151 Ex. 250
Prof. Dr. Manal A.A. Essa Depatment of Pathology	Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef 62511, Egypt	essa_manal@yahoo.com Tel. :(+20) 0822-32-7982 Mobile:(+20) 1031-09-954
Prof. Dr. Mahmoud El-Begawy Depatment of Pathology	Faculty of Veterinary Medicine, Beni-Suef Uni., Beni Suef 62511, Egypt	Badway35@yahoo.com Tel. :(+20) 0822-32-7982 Mobile:(+20) 1113-99-114

List of Publications

A) BOOKS:

Studies on saprolegniasis in salmonids in Hokkaido

Mortada Hussein, Kishio Hatai

7 Dec. 20011; Edition: 1st Edition, Publisher: Lambert Academic Publishing, Germany, ISBN: 978-3-8465-9911-2

https://www.lap-publishing.com/catalog/details/store/gb/book/978-3-8465-9911-2/studies-on-saprolegniasis-in-salmonids-in-hokkaido

B) Scientific papers:

1. Hussein, M. M. A. and K. Hatai. Saprolegnia salmonis sp. nov. isolated from sockeye salmon, Oncorhynchus nerka. Mycoscience, 40, 385-389, 1999.

2. Hussein, M. M. A.; S. Wada and K. Hatai, A. Yamamoto. Antimycotic activity of eugenol against selected water molds. Aquatic Animal Health 12, 224-229, 2000.

3. Hussein, M. M. A.; K. Hatai and T. Nomura. Saprolegniosis in salmonids and their eggs in Japan. Journal of Wildlife Diseases 37 (1), 204-207, 2001.

4. Hussein, M. M. A. and K. Hatai. In vitro inhibition of Saprolegnia by bacteria isolated from lesions of Salmonids with saprolegniasis. Fish Pathology 36 (2), 73-78. 2001.

5. Hussein, M. M. A.; M. A. El-Feki; K. Hatai and A. Yamamoto. Inhibitory effects of thymoqinone from Nigella sativa on pathogenic Saprolegnia in fish. Biocontrol Science, 7(1), 31-35, 2002.

6. Hussein, M. M. A. and K. Hatai. Pathogenicity of Saprolegnia species associated with outbreaks of salmonid saprolegniosis in Japan. Fisheries Science 68, 1067-1072, 2002.

7. El-Feki, M.; K. Hatai and M. M. A. Hussein. Chemotactic and chemokinetic of Saprolegnia parasitica toward different metabolites and fish tissue extracts. Mycoscience, 44, 159-162, 2003.

8. Hussein, M. M. A. and W. H. Hassan. Antibacterial activity of eugenol against selected fish pathogenic bacteria. Proc. The 1^st Intern. Conf. Vet. Res. Div., NRC, Cairo, Egypt. pp. 356-364, February, 2004.

9. Hussein, M. M. A. Contribution towards introduction of eugenol as a fish anesthetics in Carp, Cyprinus carpio, fish hatcheries in Egypt: Field trials. Proc. 11^th Sci.Cong. Fac. Vet. Med., Assiut Univ., Egypt. 2004

10. Hussein, M. M. A. and K. Hatai. Multiplex PCR for detection of Lactococcus garvieae, Streptococcus iniae and S. dysgalactiae in cultured Yellowtail. Aquaculture Science 54 (3), 269-274. 2006

11. Hussein, M. M. A. and K. Hatai. Chronic mortalities in cultured yellowtail, Seriola quinqueradiata (Temminck and Schlegel) and amberjack, Seriola dumerili (Risso), during winter due to streptococcosis in southern Japan. Egyptian journal of Aquatic Biology and Fisheries 11 (3), 817-832. 2007.

12. Abd El Rahman, A. H.; Abd El Galil, M. A.; Kamel, H. H. and Hussein, M. M. A. Effect of saprolegniosis on Oreochromis niloticus. Egyptian journal of Aquatic Biology and Fisheries 11 (3), 749-761. 2007.

13. M. M. A. Hussein, W. H. Hassan. Efficacy of fosfomycin in controlling streptococcosis in Nile tilapia (Oreochromis niloticus). Beni Suef Veterinary Medical Journal 21 (1). 59-66. 2011

14. M. M. A. Hussein, W. H. Hassan, I. M. I. Moussa. Potential use of allicin (garlic, Allium sativum Linn, essential oil) against fish pathogenic bacteria and its safety for monosex Nile tilapia (Oreochromis niloticus). Journal of Food, Agriculture & Environment. 11 (1): 696-699 . 2 0 1 3.

15. Hussein, M. M. A., Hassan W. H. Maha A. M. Pathogenicity of Achlya proliferoides and Saprolegnia diclina (Saprolegniaceae) Associated with Saprolegniosis Outbreaks in Cultured Nile Tilapia (Oreochromis niloticus). World Journal of Fish and Marine Sciences 5 (2), 188-193. 2013.

16. Ebied, S. K. M., Ali, H. A., Hussein, M. M. A. Studies on some pathogenic bacteria in cultured Nile tilapia (Oreochromis niloticus) with special reference to Aeromonas hydrophila and its sensitivity to different antimicrobial agents and some herbal extracts. Animal Health Research Journal 4 (1), 340-355. 2016.

17. Hussein, M. M. A.. DNA vaccination against streptococcosis. Under the process of submission.

C.V PDF

Master Title

Some studies on gills affections of fresh-water fishes

Master Abstract

The present study was carried out on 112 Tilapias and 43 Armout catfishes collected from Nile resources and Ibrahimia branch at Beni Seuf governorate during different seasons. All fishes were subjected for clinical examination. The results of clinical examinations revealed the presence of clinical abnormalities in 78 Tllapia and 21 Armout catfishes- These abnormalities includes sluggish movements, loss of condition, signs of dullness, loss of scales balance, detachment of scales, paler or darker color than normal, erosions, ulceration in different parts of: the body. Sometimes there were signs of asphyxia, accumulation of mucous in gill pouches and sometimes yellowish creamy cysts were present in the gills. All employed fishes were subjected for parasitological examination of the gills. The results revealed detection of different types of parasites including, Chilodenella species, Trichodina species, Ichthyophithirus species, Epistylls species. Mongenetic trematodes and lambroglena species in Tilapia fishes. Armout catfishes were infested with Chilodenella species, Trichodina species, Ichthyophithirus species, Henneguya species and Monogenetic trematodes. Concerning the seasonal variation of the investigated parasites, the results revealed highest incidence during winter, followed by spring and autumn, while the lowest incidence was recorded in summer. In this study the gills of all employed fishes were subjected for bacteriological examination. The results revealed isolation of different types of bacteria including: Aeromonas hydrophila, Pseudomonas fluoresces, E.Coli, Citrobacter, Staph, Staph aureus. Tmonots, A .hydrophila and Pseudomonas fluorescence predominate in diseased fishes than in a apparently healthy fishes. Application of antibiotic sensitivity teat to Aeromonas hydrophila revealed high sensitivity to "oxytetracyclin (1007.> and Chloraniphenlcol (88.87.), while Pseudomonas fluorescence was <100X) sensitive to Oxytetracyclin and Chloramphenicol. Mycological examination of gills of all employed fishes revealed the isolation of several types of moulds including Aspergilttis flavus, Aspergillus' niger, Aspergilttis flavus. Aspsrgillus ochoracious, Aspeirgiltus terreus, Penicillium species, Mucor species, Cladosporium species, Alternaria species, Absidia species and Fusariuro species. Concerning yeasts IS isolates were isolated 2 of them were identified as Candida albicans and 2 as Rhoduterul1ae species and 11 isolates were un typed. Application of antibiotics sensitivity test revealed that both types of yeasts were only sensitive to Nystatin and Clotrimazol. Concerning the relationship between different gill affections (parasitic, bacterial and mycotic). The results indicate the prevalence of parasitic affections as compared with bacterial and mycotic affection. Moreover, parasitic affections seems to predispose for other affections as incidence of parasitic - bacterial affections, parasitic - mycotic affections and parasitic - bacterial - mycotic affections were (1.6X),- (33%3, ie <16.87.) respectively. While The incidence of bacterial affections alone was (5.230 and the incidence of mycotic affections alone was (0.07.). In this study histopathological examination of gills infested with different types of parasites were carried out. The results indicate the severe damage caused by these parasites and different types of tissue alterations which is considered also as predisposing factor for other affections.

Master Abstract PDF

PHD Title

Studies on saprolegniasis in salmonids in Hokkaido

PHD Abstract

Outbreaks of water-borne fungal infections of fish, amphibians, and reptiles are a common problem, especially in fish farms and hatcheries. Of particular concern is saprolegniasis, which is an infectious fungal disease that is widespread in all stages of the life cycle of fish. The disease causes serious losses in fish farms and hatcheries, especially among salmonid species. The overall aims of this study are to gain a clearer understanding of the incidence and prevalence of saprolegniasis especially among salmonid fishes in Japan. Therefore, the author attempted to work on the objectives to investigate the Saprolegnia species associated with fungal infection in salmonids, with the goal of finding additional taxonomic criteria for such pathogenic species. Moreover, special efforts were done to develop devices for control of such infection either by using antifungal agents as substitute for malachite green and formalin or by biocontrol technique. In chapter 2, the prevalence of Saprolenia species associated with the epizootics of saprolegniasis that occurred in freshwater-cultured salmons and their eggs at some hatcheries in Hokkaido (Japan) were investigated. In almost all cases, the initial clinical sign was characterized by the growth of cotton-like mycelia on the body surface, especially the head, adipose fin, and caudal fin, but the mycelia were not visible to the naked eye in the internal organs. As a result 42 isolates, all of the genus Saprolegnia, were isolated from lesions and classified in the genus Saprolegnia according to their morphological and biological characteristics on hemp seed cultures at various temperatures. Saprolegnia salmonis was a new species added to the genus as well as the most widely and dominant species in all sites investigated in Hokkaido. Other Saprolegnia species were found to be involved in saprolegniasis incidence (in Hokkaido) and identified as Saprolegnia parasitica and as S. australis. Chapter 3, describes the pathogenicity and the pathology of of Saprolegnia salomonis NJM 9851 and S. parasitica NJM 9868, isolated from saprolegniasis outbreaks, against immature stages of five salmonids species belonging to three genera. The investigation was carried out on fingerlings of rainbow trout, Oncorhynchus mykiss (Walbaum), masuo salmon, O. masou (Brevoort), sockeye salmon, O. nerka (Walbaum), brown trout, Salmo trutta (Linnaeus), and Japanese char, Salvelinus pluvius (Hilgendorf). The cumulative mortality rates of the tested fish groups that exposed to high zoospore concentrations of S. salomonis NJM 9851 were 90, 93.3 and 100% for brown trout, sockeye salmon and the other tested salmonid species, respectively. On the other hand, all salmonid species exposed to high zoospore concentrations of S. parasitica NJM 9868 experienced cumulative mortality of 100%. The histopathological changes of the saprolegniasis lesions found in all sites of infection were loss of the epidermis, edema of the hypodermis and different degrees of degenerative changes in the underling musculature. It is clear from these results that S. salmonis NJM 9851 and S. parasitica NJM 9868 are highly pathogenic Saprolegnia species to three genera of salmonid fishes. Chapter 4, aims to examine the possibility of using alternative antifungal agents of natural origin that may have no impact either on fish or on ecosystem as substitutes for other harmful ones. Eugenol, a major essential oil of clove, and thymoquinone, one of the major carbonaceous compounds of Nigella sativa oil, were investigated for their antimycotic activity against some fish pathogenic species of Saprolegniaceae as well as the toxic effect of both compounds on selected fishes. Two eugenol solutions, eugenol 10% v/v solution in dimethyl sulfoxide (DMSO) and FA 100®, which contains 10 % v/v eugenol were used. On the other hand, thymoquinone solution was prepared in tween distillated water (TDW, 25µL/mL) (v/v). The fungal species investigated for the antimycotic effect of eugenol were Saprolegnia parasitica, S. diclina, S. ferax, S. salmonis, Aphanomyces piscicida, and Achlya klebsianna while the antimycotic effect of thymoquinone was investigated only on Saprolegnia parasitica H 2 and S. salmonis. The minimum inhibitory concentrations (MICs) of eugenol in DMSO against Saprolegnia spp., A. klebsiana and A. piscicida were 500, 250, and 125 µg/mL, respectively, while the fungicidal effects (complete killing) were 1,000, 500, and 250 µg/mL respectively. In contrast, the MICs of FA100® against S. parasitica, other Saprolegnia spp., A. klebsiana, and A. piscicida, were 250, 125, 250, and 63 µg/mL, respectively, while the fungicidal effects were 1,000, 1,000, 1,000, and 63 µg/mL, respectively. On other hand, The MICs of thymoquinone against Saprolegnia parasitica H2 and S. salmonis were 125 and 63 µg/mL, respectively, while the fungicidal effect for both species was 160 µg/mL. Zoospores of the Saprolegnia spp. tested and of A. klebsiana could not germinate in the presence of 250 µg/mL of both eugenol solutions, while those of A. piscicida were killed by 125 µg/mL of both eugenol solutions. Tested zoospores of S. parasitica H 2 and S. salmonis could not germinate in 4 µg/mL of thymoquinone when they exposed for 30 minutes. The toxicity of eugenol was higher than that of thymoquinone to salmonids but both compounds were less toxic to cyprinids. Chapter 5, deals with selecting of some natural competitors (bacteria) of Saprolegnia that may be used for biocontrol of Saprolegnia infection in fisheries field. In this study 5 of 47 bacterial isolates were found to be have the capability to inhibit the growth of Saprolegnia parasitica H2 and S. salmonis NJM 9851 on solid media. The author also examined culture media for these bacteria that were effective in enhancing this activity. Among 8 media examined, BHI, HI, and to a lesser extent NA agar were suitable for demonstrating the antifungal activity. The inhibitory activity against the tested Saprolegnia spp. did not reside in supernatant of bacterial broth culture of the selected strains. The strains, which produce antifungal substances, were found to be belong to the genera Alteromonas, Pseudomonas, and Aeromonas. These results suggest that the selected bacteria are natural competitors of Saprolegia, and may be used for biocontrol of Saprolegnia in aquaculture.

PHD Abstract PDF

Mortada Mohamed Abdel-Hamid Hussein

Basic Informations

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Contact Information

Studies on saprolegniasis in salmonids in Hokkaido

4. Hussein, M. M. A. and K. Hatai. In vitro inhibition of Saprolegnia by bacteria isolated from lesions of Salmonids with saprolegniasis. Fish Pathology 36 (2), 73-78. 2001.

Master Title

Master Abstract

PHD Title

PHD Abstract