Basic Informations

C.V

 Curriculum Vitae

• Personal Data

• Occupational Background
  • Bachelor in Pharmaceutical Science from Faculty of pharmacy, Beni-Suef University, Egypt, general grade excellent with honor degree (May-2012)
  • Demonstrator at Pharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Egypt (2013 - until now)
  • Member in the committee of NMR Unit, Faculty of Pharmacy, Beni-Suef University (12-2016 till now)

• Education and Qualifications

Pre-master courses       (2014)

Courses include (Chromatography, Spectroscopy, Medicinal Plant and Plant Tissue Culture).

• Training and workshops attended

2010   Communication Skills Program from Dawaa Smart Pharma Company

2011  Interviewing skills, C.V writing, Patient Counseling and Pharmaceutical career path

2011       OTC problems & Patient Counseling by “CCHR”

2011        Selling Skills & C.V writing by “SIGMA”

2011       Marketing Skills by “BORG”

2015       NMR Spectroscopy “Theory and Application”

2016   The symposium of “Red sea brine pools: A factory for the identification of unique. Beni-Suef Universirty, Egypt.

2016   Training in the NMR Unite (From Sample preparation to Data processing), Faculty of Pharmacy, Beni-Suef University

2017    Workshop on using X ray crystallography in structural biology, Faculty of Pharmacy, Beni-Suef University.

                        

• Conferences

2017     Participation with Poster presentation Session in 1^st International Conference of Pharmaceutical and Medical Science, Faculty of Pharmacy, Suez Canal University, Egypt

• Achievements

2017    Best poster presentation award in 1^st International Conference of Pharmaceutical and Medical Science, Faculty of Pharmacy, Suez Canal University, Egypt

• Personal Activities

2016      Participating in Organizing Committee of First Art Gallery, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt

2017         Participating in Organizing Committee of Workshop on using X ray crystallography in structural biology, Faculty of Pharmacy, Beni-Suef University.

2017     Participating in Organizing Committee of International Fame Lab competition, Conference center, Beni-Suef University, Beni-Suef, Egypt

·        References

- References and letters of recommendation are available on request.

Master Title

Bioactive Secondary Metabolites from the River Nile Derived Microbes

Master Abstract

Objective: The main mission of this study is to enhance the River Nile resources and to investigate the unexplored River Nile habitat since we found that the literature to date showed little information about the chemistry and biology of the River Nile derived microorganisms. So it was claimed to isolate a number of microorganisms, using the modern hyphenated techniques for chemical profiling of crude extracts of some isolates which were also evaluated for their antimicrobial, antimalarial, antileishmanial and antitrypanosomal activities. also isolation of major bioactive constituents from River Nile derived fungus Aspergillus awamori. Methods: A number of microorganisms has been isolated, Ultra-pure DNA was extracted from some bacterial cultures for identification using bacterial 16S rRNA and fungal 18S rRNA gene sequences and using LC-HR-ESI-MS spectroscopy for chemical profiling of crude extracts of selected isolates which were also subjected to in-vitro antimicrobial, antimalarial, antileishmanial and antitrypanosomal assays. On the other hand different chromatographic techniques were used for isolation of bioactive metabolites from large scale fermented broth of the River Nile derived fungus Aspergillus amowri; the structure of the isolated compound was elucidated based on spectroscopic analysis. Results: Success of isolation of about 34 versatile isolates was achieved, from which seven were successfully identified. The ethyl acetate (EtOAc) extracts of one isolate (RN-008) showed activity against Methicillin-resistant Staphylococcus aureus (MRSA) (IC50 =167.2248µg/ml) and against VRE (IC50 = 93.514µg/ml). Also (RN-008 & RN-003 ) showed activity against both Plasmodium falciparum D6 and P. falciparum W2 (IC50=21.009 & 25.939 and 16.283 & 27.278 µg/ml, respectively). Some isolates showed good antitrypanosomal activity (IC50 of RN-205,RN-008 and RN-011 were <0.8, <0.8 & 0.96 µg/ml respectively). Four compounds had been isolated from the fungus Aspergillus awamori; the aromatic ester di-(2-ethylhexyl) Phthalate (DEHP), one steroid compound; ergosterol and two unsaturated fatty acids notably; a-oleic and a-linoleic acids. The major isolated metabolite, DEHP, exhibited prominent activity against Candida albicans (zone of inhibition= 20 mm) fungi and Gram positive bacteria Sarcina lutea (zone of inhibition= 23 mm). It also showed cytotoxic activity against some carcinoma cell lines (human breast carcinoma (IC50= 6.525 µg/ml), human liver carcinoma cells (IC50= 26.73 µg/ml) and human cervix carcinoma cells (IC50= 42.2958 µg/ml)). Conclusion: The River Nile could be considered as a great source for bioactive metabolites and intriguing subject for further studies. Keywords: River Nile, Aspergillus, 16S rRNA, LC-HRESIMS, antimicrobial, cytotoxic, antiprotozoal.

PHD Title

PHD Abstract