Basic Informations
C.V
CURRICULUM VITAE
Asmaa Nady Mohammed, PhD
Basic Information
Name: Asmaa Nady Mohammed Mohammed
Surname: Mohammed
Nationality: Egyptian
Birth Data: August 29, 1982, Nasser, Beni-Suef, Egypt.
Phone: +2 01227525459
Fax: +2 082 2327982
E-mail: asmadel82@yahoo.com asmaa.mohamed2@vet.bsu.edu.eg
Work Address: Department of Hygiene, Zoonoses and Epidemiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef , Egypt .
Marital Status: Married
Biography
Education (academic background):
1- PhD in veterinary science, Animal, Poultry and Environmental Hygiene, (24/4/2012), Beni-Suef University, Egypt.
2- Master in veterinary medicine, Animal, Poultry and Environmental Hygiene ( (29 /6 /2008 Beni-Suef University, Egypt.
3- Bachelor of Veterinary Medical Science (May 2004), Cairo University, Beni-Suef branch, Egypt (very good).
Professional experiences (occupations):
From January 2005 to May 2008:
Demonstrator of Animal, Poultry and Environmental Hygiene, Department of Hygiene, Zoonoses and Epidemiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef , Egypt .
From August 2008 to March 2012:
Assistant lecturer of Animal, Poultry and Environmental Hygiene, Department of Hygiene, Zoonoses and Epidemiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef , Egypt .
From April 2012 until now:
Lecturer of Animal, Poultry and Environmental Hygiene, Department of Hygiene, Zoonoses and Epidemiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef , Egypt .
Academic teaching & responsibilities:
1- Teaching and demonstrating practical classes of Animal, Poultry and Environmental Hygiene for 5th year students from May 2012 until now.
2- A staff member in summer training program for academic undergraduate students, from July 2012 until now.
3- Teaching theoretical lectures and practical class of Animal Poultry and Environmental Hygiene for 5th years students from May 2012 until now.
4- Teaching theoretical lectures and practical class of Animal Poultry and Environmental Hygiene for postgraduate students from May 2014 until now.
5- External examiner for under-graduate students in other faculties of Veterinary Medicine in Egypt.
Scientific activities:
1- Co-author of Animal, Poultry and Environmental Hygiene, 5th year students book (four parts).
2- Supervisor of students at diploma, master and PhD degree (Animal, Poultry and Environmental Hygiene).
3- Assessment and peer review of papers submitted to some local and international journals:
-Journal of Veterinary Medicine and Animal Health
- Beni-Suef University journal of Basic and applied science.
- Journal of Parasitology and Vector Biology.
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4- Contributing in the committee of the department of Hygiene, Zoonoses and Epidemiology to upgrade the courses (course specification) of Animal, Poultry and Environmental Hygiene for under-graduate and post–graduate students in Beni-Suef University.
5- Concerning published papers and international book project, look to the list of publications.
Projects:
1- PI of: Risk assessment of hygienic quality and sanitizer's efficacy of drinking water in livestock farms in Beni-Suef Governorate. Projects Funding and Granting Unit at Beni-Suef University, Egypt.
2- Program for mastitis resistance and raise the quality level of milk in Beni-Suef province as a model to reduce waste and improve the safety of the production rates. Financing for the project (Academy of Scientific Research and Technology implementation of innovative initiatives).
3- Assessment of cost effective techniques for brood detection and treatment of waterborne pathogens in Drinking water using adsorptive method on Kaolin clay and its modified forms. Financing for the project: Projects Funding and Granting Unit at Beni-Suef University, Egypt.
Awards and shields:
1- University award for obtaining the competitive university project entitle" Risk assessment of hygienic quality and sanitizer's efficacy of drinking water in livestock farms in Beni-Suef Governorate.
2- University award for international scientific publication entitle" Field study on evaluation of the efficacy and usability of two disinfectants for drinking water treatment at small cattle breeders and dairy cattle farms".
3- University award for international scientific publication entitle" Ecological study on antimicrobial-resistant zoonotic bacteria transmitted by flies in cattle farms".
4- University award for international scientific publication entitle" Impact of Seasonal Conditions on Quality and Pathogens Content of Milk in Friesian Cows".
5- Award from Faculty of veterinary Medicine, Beni-Suef University (2014) for obtaining the university project entitle" Risk assessment of hygienic quality and sanitizer's efficacy of drinking water in livestock farms in Beni-Suef Governorate.
6- Award of distinction from Faculty of Veterinary Medicine, Beni-Suef University for obtaining the degree of PhD entitle" Bovine mastitis epidemiology; practical approaches and applications.
7- Award of distinction from Faculty of Veterinary Medicine, Beni-Suef University for obtaining the degree of Master entitle "Hygienic studies on water quality in fish farms and its effect on performance.
8- Award of distinction from Faculty of Veterinary Medicine, Beni-Suef University for obtaining the degree of Bachelor in Veterinary Medicine (very good).
9- Shield Association from Faculty of Veterinary Medicine, Beni-Suef University for obtaining the faculty on "first center in festival of student activities at the Beni-Suef University level.
10- Award of distinction from Faculty of Veterinary Medicine, Beni-Suef University for contributing in the development of "The Research Plan (2016-2020)".
11- Award of distinction from Faculty of Veterinary Medicine, Beni-Suef University for contributing in the design and foundation of "The website of “Journal of Veterinary Medical Research". The official veterinary journal of Beni-Suef University during the period of Oct 2015- Jan 2016.
12- Award of the 8th scientific conference of faculty of veterinary Medicine, Beni-Suef University "Shield of the scientific conference" for the conference organization during the period of 25-29 October 2016.
Membership
1- Egyptian Society for Animal Welfare.
2- Egyptian Society of Veterinary Parasitology.
Conferences, seminar, symposium, training courses and workshops
Conferences
- African Forum for Asia II and Egyptian. The Egyptian Association for OIE, Cairo conference hall of Al-Azhar University held from 20 to 21 November 2012.
- Participation in the University Conference (towards building strategies research of the university in the next decade), held from 12 to 13 November 2013, paper entitled: "Obstacles and challenges of community participation in the field of scientific research in the Arab Republic of Egypt."
- Participation in the First International Conference of distance education and its role in the development of university education system of the Arab world "in conjunction with the Association of Arab Universities research entitled" between the constraints and the future of e-learning in Egypt "in the period 17-18 December 2013.
- Participation in the Fourth International Conference of Beni-Suef University in conjunction with the Association of Arab Universities about the role of private businesses and civil society in the elevation people, paper entitled "Towards achieving an effective partnership between the Egyptian universities and the private sector held from 18 to 19 February 2014.
- "Research endowment of the Beni-Suef University conference: its controls, its resources and its banks" held on Tuesday, 4 March, 2014.
- Participation in the organization and attend the First Scientific Conference of the Department of Hygiene, Management and zoonoses lecture entitled " Hygiene, Management and zoonoses department between reality and expectations" Tuesday, April 8, 2014.
- The University Conference: Excellence: standards and social tasks and return. Tuesday, 17 June 2014 centers.
- Training and development second national Conference on Higher Education: "fired your talent and possessed the future." held on November 18, 2014 at Beni-Suef University.
- The 2th conference of people with disability entitled: our youth are not energy held on Tuesday, December 2, 2014 University
10. The 2th international conference on "Distance education in Arab universities' applications and future prospects" held in the period from December 16 to 17, 2014.
11. International Conference of Beni-Suef University in collaboration with the Union of inventors Egyptians titled "Egypt invent" held on March 8, 2015 .
12. Plagiarism in academic research (phenomenon and its treatment) held on November 17, 2015 at Beni-Suef University .
13. Tenth Conference of the Faculty of Arts "Towards a strategy for sustainable development in Upper Egypt in light of the humanities," held in the period from March 13 to 14, 2016 at Beni –Suef University.
14. Fifth International Conference of the Arab Federation for Sustainable Development and the Environment, entitled: The future of energy and water system, food and climate change in the Arab region in the period from March 15 to 16, 2015.
15. The Conference first to students of the Arts Faculty, "student activities and their role in the development of creativity and pioneering thinking held on Sunday, March 27, 2016 at Beni-Suef University.
16. First Scientific Conference of the education sector and Student Affairs at Beni-Suef University, "the development of educational and student services for universities in the light of the challenges of the labor market (the experiences and future visions) Learn trained and competed complex during the period from 18-19 April 2016.
17. Scientific Conference towards building a strategy to transform the student to the researcher, held on 8 November 2016.
- Participation in the organization and presenter in the eighth scientific conference of faculty of Veterinary Medicine Beni-Suef University entitled "The health risks that threaten human and animal health Challenges and Opportunities " held in the period from 25 to 29 October, Hurghada.
Seminar and symposium
1. Modern tools of nutrition held on 6th December 2015 in Faculty of Veterinary Medicine, Beni-Suef University.
2. Ethics and guidelines of animal use in scientific research held on 8th December 2015 in Faculty of Veterinary Medicine, Beni-Suef University.
3. Seminar on how to write research proposals, held at the Faculty of Pharmacy, Beni-Suef University 2013.
2. Seminar "Evaluation of FMD combating measures in Egypt" held on Tuesday, 27 November 2013 at the Faculty of Veterinary Medicine, Beni-Suef University.
3. Seminar "The current epidemiological situation of Avian Flu in Egypt, held on Tuesday, December 17 2013 at the Faculty of Veterinary Medicine.
4. Seminar " Biodiversity in the Sinai, which was organized by the National Committee of Biological Sciences held in 23, 2014 November 2014 at the National Research Center.
5. Seminar postgraduate research sector entitled "mechanisms to encourage international publication" held in Tuesday, 5August 2014.
6. Participate in a symposium postgraduate research sector entitled "The effectiveness of subsidized university" held on Sunday, 24th August 2014.
7. Participate in postgraduate research sector, a seminar entitled "Promoting Patents: standards and evaluation" held in Tuesday, September 2, 2014.
8. Seminar mechanisms for international publication held on August 5, 2014 at Beni-Suef. University.
9. Participation in postgraduate sector research seminar entitled "Competitiveness projects: effective sources of funding for scientific research from the outside." held on Tuesday, 16 September 2014 at Beni -Suef University
10. Biological Diversity in the Sinai, which was organized by the National Committee of Biological Sciences, which was held on Sunday, November 23, 2014.
11. Discovery and development of creative thinking held on Tuesday, December 1, 2015 at Beni-Suef University.
12. Towards a strategy for effective management of the university students on scholarships and held on Tuesday, August 18, 2015 at Beni- Suef University.
13. Seminar of the research and scientific cooperation between the dimensions of the Korean Cultural Center and the University of Beni Suef, held on Tuesday, 8 September 2015.
14. Seminar research capacity of the university and its role in solving industrial production and service institutions and the problems of the community held on Sunday, 13 September 2015.
15. Symposium rules upgrades professors and assistant professors held on Thursday, 14 May 2015.
16. Seminar programs and awarded the German Academic Exchange held on Tuesday 26 protection of 2015.
17. Mechanism to attract professors and Zairian held on Sunday, November 8, 2015 at Beni-Suef University.
18. Seminar Alexander von Foundation Hombldt and access to research grants for post-doctoral studies in Germany, held on Tuesday, May 24, 2016.
19. Seminar Information Technology: a step to upgrade the educational process held on 28 August 2016 at Beni-Suef University.
20. Seminar for the deployment of scientific research bases in the Journal of Veterinary Medicine, held on Sunday, September 4, 2016 at Beni-Suef University.
21. Good researcher and publication of the international symposium held on September 18, 2016 at at Beni-Suef University.
22. Seminar how to prepare the research project proposals writing steps and finance held on Tuesday, October 4, 2016 at Beni-Suef University.
Training courses & Workshops
- Literature and Ethics (15 hours) held at Beni-Suef University 2006.
- Effective Presentation Skills (15 hours) held at Beni-Suef University 2006.
- Systems of credit hours (15 hours) held at Beni-Suef University 2007.
- Using of technology in teaching (15 hours) held at Beni-Suef University 2007.
- Systems of examinations and student assessment (15 hours) held at Beni-Suef University, July 2011.
- Organization of scientific conferences (15 hours) held on July 2011 at Beni-Suef University.
- Research projects and competitive (15 hours) held at Beni-Suef University, July 2011.
- Financial and legal aspects (15 hours) held at Beni-Suef University, July 2011.
- University administration (15 hours) held at Beni-Suef University, February 2012.
- Courses specification (5 hours) held at Beni-Suef University, December 2012.
- Self-evaluation (5 hours) held at Beni-Suef University, December 2012.
- Quality standards in the teaching process (15 hours) held at Beni-Suef University, March 2012.
- Socioeconomic impact of climatic change on sea level rise held from 17 to 18 October 2012, in Alexandria Research Centre for Adaptation.
- Relationship between scientific research and agriculture sector in Egypt “problems and solution” held on 17April 2013, at Faculty of Agriculture, Fayoum university.
- Team building for team leader (8 hours) held on April 2013, DAAD Kairo Akademie.
- Recent techniques in Molecular Biology held on 7&8 May 2014, in Faculty of Veterinary Medicine, Beni-Suef University.
- Nano- biotechnology training course held from 3th to 5th March 2015 at the faculty of postgraduate studies for advanced science, Beni-Suef University.
- Biostatistics and data analysis held for 3 days on April 2015 at faculty of science, Beni-Suef University.
- Water analysis in animal, poultry and fish farms held from 26 April to 5 May 2015 at Faculty of veterinary medicine, Beni-Suef University.
- Scientific thinking and argumentation skills for problem solving held on 22 December 2015, DAAD Kairo Akademie.
- Introductory course on Egyptian knowledge bank at the University of Beni-Suef, held on 13 March 2016.
- Proposal writing post docs held on 26 April 2016, DAAD Kairo Akademie.
- International publishing held on 6th of September 2016, DAAD Kairo Akademie.
- Scientific writing and publishing, parasites of farm animals, impact of parasitic disease for the veterinarian held on 11 to 14 July, 2016 at faculty of veterinary medicine, Beni-Suef University.
- Workshop clinical key database, digital library, digital communication windows of the sources of scientific and global information work held on 16 October 2016 at Beni-Suef University.
- International publishing scientific research. Center of Human resource Development, Beni-Suef University, held in the period from August 23 to 24, 2015.
- Communication skills in the education process. Center of Human Resources Development, Beni-Suef University, held in the period from August 25 to 26, 2015. (15hours).
- The research team management. HRD Beni-Suef University, held in the period from August 9 to 10, 2015. (15 hours).
- Strategic planning. Center of Human Resources Development, Beni-Suef University, held in the period from November 8 to 9, 2016. (15 hours).
- Quality standards in the teaching process. Center of Human Resources Development Beni-Suef University, held on November 15 -16, 2016. (15 hours).
- University administration. Center of Human Resources Development Beni-Suef University, held in the period from November 27 to 28, 2016. (15 hours).
List of Publications
1. Asmaa, N. Mohammed (2008): Hygienic studies on water quality in fish farms and its effect on fish performance. Master Degree Thesis . Faculty of Veterinary Medicine, Beni-Suef University, Egypt
- ELBably, M.A.; Emeash, H.H.; Asmaa, N.M. and Nahla.R. (2008): Influence of water quality on fish productivity. Beni-Suef- Vet.Med.J.
- Emeash, H.H.; ElBably.M.A.; Asmaa, N.M. (2008): Effect of seasonal variation on performance and parasitic infestation of cultured fish in Fayoum governorate . Beni-Suef- Vet.Med.J.
- Asmaa, N. Mohammed (2012): Bovine mastitis epidemiology practical approaches and applications. PhD degree Thesis, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.
- El Bably, M.A.; Emeash, H.H. ; Asmaa, N.M. (2013) : Risk factors associated with mastitis occurrence in dairy herds in Beni-Suef , Egypt. World's Veterinary Journal (WVJ). 3 (1), 5-10.
- Asmaa, N.M.; El Bably, M.A.; Shokier, Kh. A (2014): Studies on prevalence, risk indicators and control options for tick infestation in ruminants. Beni-Suef Univerisity Journal of basic and applied science XXX (1-6).
- Asmaa N. Mohammed (2014): A potential approach for monitoring and evaluation of drinking water sources and quality for livestock animals at Beni-Suef province. Global Veterinaria 13 (4): 534-543.
- Asmaa N. Mohammed (2014): Assessing the Antimicrobial Resistance Patterns and in Vitro Efficacy of three Disinfectants as teat dip against Iodine (0.5%) Resistant Mastitogenic Pathogens. International Journal of Advanced Research 2 1)2( 946-953.
9. Asmaa N. Mohammed; R.L. Abdel Aziz; M.M.A. Zeinhom (2015): Exploitation of multiple approaches to adapt and mitigate the negative effects of heat stress on milk production and fertility of Friesian cows under field conditions. Assuit Vet. Med. J. 61 (1 47 ): 3 3 -4 2 .
10. Asmaa N. Mohammed (2016): Field study on evaluation of the efficacy and usability of two disinfectants for drinking water treatment at small cattle breeders and dairy cattle farms. Environ. Monit. Assess. (188):151.
11. Asmaa N. Mohammed1; Gihan K. Abdel-Latef1; Naglaa M. Abdel-Azeem1; Khaled Mohamed El-Dakhly2 (2016): Ecological study on antimicrobial-resistant zoonotic bacteria transmitted by flies in cattle farms. Parasitol Res; 2016, 115:3889–3896.
12. Hassouna1 M.E.M.; ElBably2 M.A.; Asmaa N. Mohammed2*; Nasser1 M.A.G. (2016): Assessment of carbon nanotubes and silver nanoparticles loaded clays as adsorbents for removal of bacterial contaminants from water sources. Journal of water and health. DOI: 10.2166/wh.2016.304.
13. Mohamed M. A. Zeinhom*1; Rabie L. Abdel Aziz2; Asmaa N. Mohammed3; ; Umberto Bernabucci4 (2016): Impact of seasonal conditions on quality and pathogens content of milk in Friesian cows. Asian-Australasian Journal of animal sciences; 2016, 29(8): 1207-1213.
14. Asmaa N. Mohammed; Naglaa M. Abdel Azeem ; Gehan K. Abdel Latef (2016): Field Trial using Combined Treatment of Garlic and Organic Spray Formula for File's Control and Animal Defensive Behavior Alleviation in Cattle Farm. Asian Journal of Animal Sciences.10 (6) 280-289.
15. Abdou Kh. A., Walaa A. Moselhy, Asmaa N. Mohammed, Abulgassm M.A., Khadiga I. Ahmed (2016): Monitoring the hygienic quality of underground water in different localities in Egypt and Libya. Journal of Veterinary Medical Research 2016, 23 (1): 25-34.
16. Asmaa N. Mohammed1* and Hassan El Sayed Helal2 (2016): Current situation assessment of biosecurity measures of some poultry sectors and hatcheries in Egypt. Journal of Veterinary Medical Research 2016, 23 (2):
Review Articles:
1- El Bably, M.A. and Asmaa, N.M. (2013): An assessment of the likelihood of the introduction of new strain of foot and mouth disease virus to Egypt. 117th USAHA and 56th AAVLD Annual Meeting. http://aavld2013.abstract central.com.
2- Asmaa N. Mohammed* and Mohamed A. ElBably (2016): Technologies of Domestic Wastewater Treatment and Reuse: Options of Application in Developing Countries. JSM Environmental Science and Ecology 4(3): 1033.
Book project:
1- Asmaa N. Mohammed (2014): Bovine mastitis epidemiology practical approaches and applications. International publishing in (Lambert academic publishing). ISBN: 978-3-659-57026-1.
2- Asmaa N. Mohammed (2016): Evaluation of water quality in fish farms and its effect on performance .International publishing in (Lambert academic publishing). ISBN: 978-3-659-87245-7.
3- E. Learning books: (Higher education Ministry, Higher Supreme of Universities, Egypt .Theoretical notes and Practical notes on Animal, Poultry and Environmental Hygiene (four parts). cms.nelc.edu.eg
List of Thesis supervision:
1. Abulgassm Massud Abulgassm (2013): Monitoring and evaluation of ground water environmental pollution in Egypt and Libya. MVSC Thesis. Faculty of Veterinary Medicine, Beni-Suef University.
2. Manar Bahaa Eldin Mohammed (2013): Ecological studies on some calf enteropathogens resistant to antibiotic and antiseptic agents. PhD Thesis. Faculty of Veterinary Medicine, Beni-Suef University.
3. Aya Mohamed El Sayed (2015): Evaluation of Environmental Hygiene and Microbiological Status of food service facilities. MVSC Thesis. Faculty of Veterinary Medicine, Beni-Suef University.
4. Islam Samy Abdel Zaher (2016): Assessment of different methods for hygienic treatment of some waste types in poultry farms. MVSC Thesis. Faculty of Veterinary Medicine, Beni-Suef University.
5. Yara Mahmoud El Sayed (2016): Epidemiological study of some pathogens of pets in veterinary clinics. PhD Thesis. Faculty of Veterinary Medicine, Beni-Suef University.
Master Title
Hygienic Studies on Water Quality in Fish Farms and its Effect on Performance
Master Abstract
A field study was carried out during the period from April 2006 till the end of October 2007 in four fish farms in different localities in Egypt to investigate the water quality of fish farms with different water sources (fresh, brackish, agriculture and ground water) and its effect on fish performance (growth & survival rate) beside parasite infestation of cultured fish. Results revealed that although the average values of physio-chemical and bacteriological parameters were within the acceptable limits at water inlets in examined farms; a great variation were recorded among both Farms and ponds within the same farms as water in ponds of farm (IV) showed a significant increase in the measurable parameters P <0.05 with a significant decrease in dissolved oxygen followed by pond 2 in farms (III) and (I) respectively (3.6±0.7, 4.9± 0.61 and 6.5±0.31 mg/l). Meanwhile farm (II) showed a significant decrease at (P<0.01) compared with other examined farms. Faecal coliforms were isolated from water ponds and fish in farms I, III and IV (1.0±0.12, 5.02±0.5, 14.0±3.6 and 23.0± 4.3/ 100ml) & (0.4±1.5, 1.6±1.5, 4.0 ±1.0, 10.8±1.2, 10.6±3.0 and 12.0±3.2). On the other hand, a significant increase P <0.01 in water temperature in accompanied by other physio–chemical and faecal coliform during summer months (June to August) subsequently resulting in a significant increase in parasite intensities and retardation in growth rate comparing with those in other months.
PHD Title
Bovine Mastitis Epidemiology; Practical Approaches and Applications
PHD Abstract
Epidemiological study was carried out in two private dairy farms of totally 350 lactating cows of different breeds, ages and distributed in Beni-Suef governorate during the period from April, 2009 till the end of November, 2011 through field screening survey by using of California mastitis test (CMT) for each quarter milk samples followed by Somatic cell count (SCC) and bacteriological examination to identify the major causative agents of intramammary infection (IMI), determining the prevalence of mastitis and related risk indicators, evaluation the in vitro sensitivity of bacterial isolates to certain antibiotics and antiseptics commonly used in veterinary practices and finally establishing a new practical approach for prevention and control of mastitis in dairy herds. Results revealed that the highest percentage of subclinical and clinical mastitis at cow level was 41.88% and 13.67% in farm (I) compared with 33.05% and 9.87% in farm (II) respectively based on bacteriological results. It was found that the most frequently major causative agents isolated were Coagulase negative staphylococci (CNS) was the prevalent organisms isolated from individual milk samples 32.21% followed by Staph.aureus 26.84% , E. coli (o157&o103) 21.48% , Strept.agalactiae 12.75% , Strept.uberis 3.35% and Klepsiella spp. 3.35% respectively .in relation to score (-) ; CNS was represented the highest percentage (83.33%) followed by Strept.agalactiae (16.67%). Meanwhile in farm (II), Staph.aureus was the second predominant isolates (26.27%) next to CNS (36.86%) compared to others (E. coli 19.49%, Strept.agalactiae 11.0%, Klepsiella spp. 3.4% and Strept.uberis 2.97%). The sensitivity of CMT was high in farm II (98.9%) compared to (98.0%) in farm II. Meanwhile specificity of test was nearly similar in both farms (98.1%) in relation to causative agent. There was a significant different (P<0.001) amongst mean SCC in all studied groups in both examined farms. when studying the seasons of year, age, lactation stage and parity factor, it was found that the highest percentage of subclinical and clinical mastitis was occurred in summer in both farms at 44 (36.06%) &18 (14.75%) in farm (I) while in farm (II) was 66 (27.5%) &21 (8.75%) . in relation to age susceptibility, younger cows (3-5 years) were more affected by mastitis in both farm than adults (6-8 years ) and older cows (>9 years) .Moreover in lactation stage, occurrence of mastitis was higher in early lactation stage in farm (I&II) 60.68% and 45.5% respectively; occurrence of mastitis in farm (I) was high in cows with parities (>4) 65.79% compared with farm (II) the highest occurrence of mastitis at cows with parities (2-4) 17.78 % respectively. Enrofloxacin was the most effective antibiotic where 96.3 % of all isolates were susceptible except Strept.dysgalactiae was intermediate. Prevention and control mastitis Program was concluded that dry cow therapy , hygienic milking procedure and post milking teat dipping have an important role in eliminating the existing and decrease new intra mammary infection from 17 (42.5 %) to 5 (12.5%) and from 19 (47.5) to 9 (22.5) in both treated groups.