Basic Informations

C.V

Sameh AbouZid C.V. 1
C.V.
Sameh AbouZid
PERSONAL DETAILS Surname
AbouZid Other names
Sameh Fekry Salama Title
Dr. Gender
Male Address (Work)
10 Ahmed Hegazy Street, Beni-Suef 62111, Egypt Tel. No.
+002-02-38303528 Mobile
+02-01006495952 E-mail
sameh.zaid@pharm.bsu.edu.eg
wssmd3000@yahoo.com Date of birth
Day
1
Mo
10
Yr
1974 Place of birth
Giza, Egypt
EDUCATION School/College/University/Other Degree obtained Date (from-to)
The University of Tokyo, Graduate School of Pharmaceutical Sciences
Ph.D. (Pharmaceutical Chemistry)
10/2000 – 9/2003
The University of Tokyo, Graduate School of Pharmaceutical Sciences
M.Sc. (Pharmaceutical Chemistry)
9/1998 – 9/2000
Cairo University, Faculty of Pharmacy
B.Sc. (Pharmaceutical Sciences)
9/1990 – 5/1996
EMPLOYMENT HISTORY Employer Position Date (from-to)
Institute of Medicinal and Aromatic Plants, Beni-Suef University
Supervisor
6/2016 - to present time
Faculty of Pharmacy,
Beni-Suef University
Head of Pharmacognosy and Medicinal Plants Department (Acting)
10/2015 - to present time
Faculty of Pharmacy,
Beni-Suef University
Vice-Dean for Education and Students' Affairs (Acting)
10/2012 - 10/2014
Faculty of Pharmacy,
Beni-Suef University
Vice-Dean for Postgraduate Studies and Research (Acting)
9/2011 - to 9/2012
Faculty of Pharmacy,
Beni-Suef University
Assistant Professor
12/2010 – to present time
Faculty of Pharmacy,
Nahda University in Beni-Suef
Lecturer
2/2009 – to present time
Faculty of Pharmacy,
Beni-Suef University
Quality Assurance Unit - Executive manager
4/2008 - 9/2011
Faculty of Pharmacy,
Lecturer
02/2005 –
Sameh AbouZid C.V. 2
Beni-Suef University
12/2010
MEMBERSHIP OF PROFESSIONAL ASSOCIATIONS Professional body Level of membership Year of award
Pharmaceutical Society of Japan
Former member
2000
Japanese Society of Plant Cell and Molecular Biology
Former member
2003
Egyptian Botanical Society
Member
2004
Egyptian British Biological Society
Member
2004
The Egyptian Society of Environmental Toxicology
Member
2005
The Egyptian Pharmaceutical Society
Member
2005
Natural Product Research Network for Eastern and Central Africa (NAPRECA)
Member
2013
American Chemical Society (ACS)
Member
2014
Research Network Natural Products against Neglected Diseases
Member
2014
Society for Medicinal Plant and Natural Product Research
Member
2016
TRAINING Training attended / Technical skills acquired Place Date (from-to)
Ethical Conduct and Code of Ethics
Center of Human Resources Development, Beni-Suef University
24/4/2016-
University Management
Center of Human Resources Development, Beni-Suef University
5/4/2016-6/4/2016
University Legal and Financial Aspects
Center of Human Resources Development, Beni-Suef University
13/3/2016-14/3/2016
Research Ethics
Center of Human Resources Development, Beni-Suef University
17/4/2016-18/4/2016
Technology in Teaching
Center of Human Resources Development, Beni-Suef University
10/4/2016-11/4/2016
The Credit Hour System
Center of Human Resources Development, Beni-Suef University
26/3/2016-27/3/2016
Guidelines for Researchers
Bibliotheca Alexandrina, Alexandria, Egypt
27/9/2010 – 30/9/2010
TOT
Faculty and Leadership Development Project (FLDP), Beni-Sueif University
30/4/2007 –
3/5/2007
Sameh AbouZid C.V. 3
Introduction to SCORM
The American University in Cairo (AUC) – Engineering Services
2006 Summer School
How to Write a Successful Research Proposal, workshop
Bibliotheca Alexandrina, Alexandria, Egypt
8-10 December, 2005
Scientific Writing Skills course
Egyptian National Agricultural Library
13/3/2004 – 22/3/2004
E-learning Content Development
The American University in Cairo (AUC) – Engineering Services
November-December, 2005
Isolation and Identification of Plant Polyphenols
National Research Centre, Cairo, Egypt
15/5/2004 – 29/5/2004
Scientific Writing Skills course
Egyptian National Agricultural Library
13/3/2004 – 22/3/2004
Mushroom Production and Marketing training course
Food and Technology Research Institute, Agricultural Research Centre, Egypt
5/2004
University Teacher Preparatory course
Cairo University
14/2/2004 – 3/3/2004
JOURNAL PUBLICATIONS (Indexed Journals)
1. Polyacetylenes accumulation in Ambrosia maritima hairy root and cell cultures after elicitation with methyl jasmonate. Sameh AbouZid and Yutaka Orihara. Plant Cell, Tissue and Organ Culture 2005, 81: 65-75.
2. Molluscicidal Activity of Polyacetylenes from Ambrosia maritima hairy roots. Sameh AbouZid, Yutaka Orihara and Masanori Kawanaka. Natural Product Communications 2007, 2 (2): 177-180.
3. Biosynthesis of polyacetylenes in Ambrosia maritima hairy root culture. Sameh AbouZid and Yutaka Orihara. Planta medica 2007, 12 (73): 1327-1329.
4. Steroidal Glycoalkaloids from Berries of Solanum distichum. Sameh AbouZid, Nermeen Fawzy, Nebal Darwish and Yutaka Orihara. Natural Product Research 2008, 22 (2): 147-153.
5. Antioxidant Activity of Wild Plants Collected in Beni-Sueif Governorate, Upper Egypt. Sameh AbouZid, Abdelaaty Elshahaat, Sajjad Ali, Mohamed I. Choudhary. Drug Discoveries & Therapeutics, 2008, 2(5): 286-288.
6. A New Ferulic Acid Ester and Other Constituents from Tamarix nilotica Leaves. Sameh AbouZid, Sajjad Ali, Mohamed Iqbal Choudhary. Chemical & Pharmaceutical Bulletin, 2009, 57(7): 740-742.
7. Withaferin A Production by Root Cultures of Withania coagulans. Sameh AbouZid, Asma Nasib, Seif Khan, Javed Qureshi, Mohamed Iqbal Choudhary. International Journal of Applied Research in Natural Products, 2010, 2(5): 23-27.
8. A New Prenylated Flavanoid with Antibacterial Activity from Propolis Collected in Egypt. Ashraf El-Bassuony, Sameh AbouZid. Natural Product Communications, 2010, 5(1): 43-45.
Sameh AbouZid C.V. 4
9. RP-HPLC method for determination of withanolides in flowers, leaves and roots of Withania somnifera. N. W. Ali, S. AbouZid, A. Nasib, S. Khan, J. Qureshi, M. I. Choudhary. Acta Chromatographica, 2010, 22(3): 473-480.
10. Evaluation of usefulness of wild plants growing in Beni-Sueif region, Upper Egypt. Sameh AbouZid. Functional Plant Science and Biotechnology, 2010, 5(SI1): 91-93.
11. Chemical and biological investigation of some Clerodendrum species cultivated in Egypt. Haytham M. Wahba, Sameh F. AbouZid, Amany A. Sleem, Sandra Apers, Luc Pieters, Abdelaaty A. Shahat. Pharmaceutical Biology, 2011, 49(01): 66-72.
12. Hepatoprotective and Antioxidant Activities of Tamarix nilotica Flowers. Sameh F. AbouZid, Amany A. Sleem. Pharmaceutical Biology, 2011, 49(04): 392-395.
13. Survey on medicinal plants and spices used in Beni-Sueif, Upper Egypt. Sameh F. AbouZid and Abdhalim Mohammed. Journal of Ethnobiology and Ethnomedicine, 2011, 7: 18-23.
14. Studies on flavanolignans from cultured cells of Silybum marianum. Ahlam Elwekeel, Sameh AbouZid, Nadia Sokkar, and Ahlam Elfishway. Acta Physiologiae Plantarum, 2012, 34: 1445-1449.
15. Enhanced accumulation of flavonolignans in Silybum marianum cultured roots by methyl jasmonate. Ahlam Elwekeel, Ahlam Elfishway, and Sameh AbouZid. Phytochemistry Letters 2012, 5: 393-396.
16. Antimicrobial activity of some Clerodendrum species from Egypt. Sameh F. AbouZid, Haytham M. Wahba, Ali Elshamy, Paul Cos, Louis Maes, Sandra Apers, Luc Pieters and Abdelaaty A. Shahat. Natural Product Research 2012, 27(11): 1032-1036.
17. Increase in Lipoxygenase Activity in Hairy Roots of Ambrosia maritima after Elicitation with Methyl Jasmonate. Sameh AbouZid, Yutaka Orihara. Plant Tissue Culture & Biotechnology Journal 2013, 23(1): 31-38.
18. Antihyperglycemic Effect of Crude Extracts of Some Egyptian Plants and Algae. Sameh Fekry AbouZid, Osama Mohamed Ahmed, Rasha Rashad Ahmed, Ayman Mahmoud, Ehab Abdella, Mohamed Badr Ashour. Journal of Medicinal Food 2014, March, 17(3): 400-406. doi:10.1089/jmf.2013.0068.
19. Authentication of Silybum marianum Varieties Using RAPD Analysis. Sameh AbouZid. Plant Tissue Culture & Biotechnology 2014, June, 24(1): 57-63.
20. Yield improvement strategies for production of secondary metabolite in plant tissue culture. Sameh AbouZid. Natural Product Research 2014, 28(23), 2102-2110. DOI: 10.1080/14786419.2014.927465.
21. Phytochemical screening and evaluation of biological activity of Calligonum polygonoides L. subsp. comosum. Ahmed HS, Moawad AS, Owis AI, AbouZid SF, Abdel-Rahman RF. Journal of Applied Pharmaceutical Science 2015, 5(3), 22-26.
Sameh AbouZid C.V. 5
22. An Active Learning Assignment to Improve Pharmacy Student's Knowledge of Herbal Medicine. Sameh AbouZid, Journal of Applied Pharmaceutical Sciences 2015, 5(3), 106-108.
23. Use of Nuclear Magnetic Resonance Spectroscopy in Analysis of Fennel Essential Oil. Sameh AbouZid. Natural Product Sciences 2016, 22(1), 30-34. 24. Solamargine production by a fungal endophyte of Solanum nigrum. El Hawary, Seham; Mohamed, Rabab; AbouZid, Sameh; Bakeer, Walid; Ebel, Rainer; Sayed, Ahmed; Rateb, Mostafa. Journal of Applied Microbiology 2016, 120, 900-911. 25. Silymarin content in Silybum marianum populations growing in Egypt. Sameh F. AbouZid, Shao-Nong Chen, Guido F. Pauli. Industrial Crops and Products 2016, 83, 729-737.
26. Flavonoids of Calligonum polygonoides and their cytotoxicity. Hayam S. Ahmed, Abeer S. Moawad, Asmaa I. Owis, Sameh F. AbouZid, Osama M. Ahmed. 2016, Pharmaceutical Biology, in press. 27. Silybum marianum pericarp yields enhanced silymarin product. Sameh F. AbouZid, Shao-Nong Chen, James McAlpine, Brent Friesen, Guido F. Pauli. 2016, Fitoterapia, 112, 136-143. 28. Silymarin and hydroethanolic extracts of Silybum marianum leaves and fruits potentially counteract diethylnitrosamine/phenobarbital-induced hepatocarcinogenesis. Osama M. Ahmed, Ayman M. Mahmoud, Sameh F. AbouZid, Nour Y. Saber. 2016, under review. 29. Anti-arthritic activity of 11-O-(4/-O-methyl galloyl)-bergenin and Crassula capitella extract against rheumatoid arthritis in rats. Seham S. El-Hawary, Rabab Mohammed, Sameh Abou zid, Zeinab Y. Ali and Ahlam Elwekeel. Journal of Pharmacy and Pharmacology, 2016, 68, 834-844.
30. . In-vitro Cyclooxygenase Inhibitory, Antioxidant and Antimicrobial Activities of Phytochemicals Isolated from Crassula arborescens (Mill.)Willd. Seham S. El-Hawary, Rabab Mohammed, Sameh AbouZid, Zeinab Y. Ali, Ahmed O. El-Gendy, Ahlam ElwekeelInternational Journal of Applied Research in Natural Products, 2016, 9 (4), 8-14. 31. Secondary metabolites from fungal endophytes of Solanum nigrum. Mohamed, Rabab; El Hawary, Seham; Bakeer, Walid; Rateb, Mostafa; AbouZid, Sameh; Sayed, Ahmed. Submitted. 32. Biosynthesis of flavonolignans produced by Silybum marianum. Sameh F. AbouZid, Shao-Nong Chen, James McAlpine, Brent Friesen, Guido F. Pauli. In preparation.
JOURNAL PUBLICATIONS (Others)
Sameh AbouZid C.V. 6
33. Increase in flavonoid content in red onion peel by mechanical shredding. Sameh AbouZid and Gamal Elsherbeiny. Journal of Medicinal Plants Research, 2008, 2(9): 258-260.
34. Pharmacognostical study on Erythrina crista-galli grown in Egypt. Mohamd AbouElfetouh, Rabie Elsofany, Sameh AbouZid, Marwa Hassan. Bulletin of Faculty of Pharmacy, Cairo University, 2008, 46 (3): 127-138.
35. Study of Flavonoid content of Cicer arietinum L. O. A. Rashwan, M. Magd, S. AbouZid* and M. A. Zaki. Bull. Fac. Pharm. Cairo Univ., 2009, 47(3): 121-127.
36. Phytochemical and biological studies on isoflavonoids from Dalbergia paniculata cultivated in Egypt. Enas Amin, Sameh AbouZid, Ahmed Seida. Pharmacologia, 2012, 3: 84-90.
37. Lipid content and antimicrobial activity of some Egyptian Fabaceae (Leguminosae) plants. Usama W. Hawas, Sayed A. Eltomy, Sameh F. Abozaed, Ghanem A. El-Hossary and Rafik M. Nassif. Journal of Medicinal Plant Research 2012, 6(44): 5604-5608.
38. Antibacterial Effects of Isoflavones Isolated from Cicer arietinum. Mohamed Zaki , Ali Zaid , Mostafa Magd and Sameh AbouZid. International Journal of Natural Products Research 2013, 2(1): 1-5.
39. Bioinformatics Assessment of Functional Genes/Proteins Involved in Obesity-Induced Type 2 Diabetes. Ehab M Abdella, Rasha R Ahmed, Mohamed B Ashour, Osama M Ahmed, Sameh F AbouZid and Aymen M Mahmoud. International Journal of Fundamental & Applied Sciences 2013, 2(1): 23-28.
40. Silymarin content in Silybum marianum fruits at different maturity stages. Ahlam Elwekeel, Ahlam Elfishawy and Sameh AbouZid. Journal of Medicinal Plants Research 2013, 7(23): 1665-1669.
41. Studies on garlic production in Egypt using conventional and organic agricultural conditions. Mai Raslan, Sameh AbouZid, Mamdouh Abdallah, Mohamed Hifnawy. African Journal of Agricultural Research 2015, 10(13), 1631-1635.
42. Composition of the Essential Oil of the Fruits of Phyllanthus emblica Cultivated in Egypt. Dalia El Amir, Sameh F. AbouZid, Mona H. Hetta, Abdelaaty A. Shahat, Mohamed A. El-Shanawany. Journal of Pharmaceutical, Chemical and Biological Sciences 2015, 2(3): 202-207. 43. Cytotoxicity of Solanum nigrum L green fruits on breast (MCF-7) and liver (HepG-2) cancer cell lines. Seham S. El-Hawary, Rabab Mohammed, Sameh F. AbouZid, Mostafa E. Rateb and Ahmed M. Sayed. The Pharma Innovation Journal 2015, 3(11), 87-89.
44. Phenolic contents and in vitro free radical scavenging activity of alcoholic extract of the fruits of Tribulus terrestris L. M. Hifnawy, S. AbouZid, Z. Ali, M. Fouda. The Pharma Innovation Journal 2015, 4(6), 92-100. 45. Silymarin and hydroethanolic extracts of Silybum marianum leaves and fruits attenuate diehtylnitrosamine/phenobarbital-induced nephrotoxicity via their
Sameh AbouZid C.V. 7
antioxidant and anti-inflammatory action. Osama Ahmed, Ayman Mahmoud, Sameh AbouZid, Nour Saber. American Journal of Biochemistry 2016, 6(2), 21-29. 46. Chemical composition and antimicrobial activity of the lipoid extract from the Fraxinus ornus (L.) seeds, family oleaceae. Seham S. Elhawary, Rabab Mohammed, Sameh Fekry AbouZid, Hossam M. Hassan and Marwa A. Taher. World journal of pharmacy and pharmaceutical sciences 2016, 5(5), 155-162. 47. Ameliorative effects of Punica granatum juice and fruit extracts against 7,12-dimethylbenz[a]anthracene and CCl4-induced cardiorenal toxicity in Albinao rats. Mohamed B. Ashour, Hanaa I Fahim, Sameh F. AbouZid, Ahmed R. Gaber, Hammed A. AbdelGaid, Osama M. Ahmed. SM Journal of Biology 2016, 2(1), in press.
48. Punica granatum mitigates 7,12-dimethylbenz[α]anthracene and CCl4-induced oxidative stress and hepatic precancerous lesions in wistar rats. Ahmed, O.M., Ashour, M.B., Fahim, H.I., AbouZid, S.F., Gaber, A.R. and Gaid, M.A.A. Indo American Journal of Pharmaceutical Research 2016, 6(9), 6493-6510.
BOOK CHAPTERS
? Silymarin, Natural Flavonolignans from Milk Thistle. Sameh AbouZid. In “Phytochemicals – A Global Perspective of Their Role in Nutrition and Health”, ISBN 978-953-51-0296-0, edited by Venketeshwer Rao, InTech - open science | open minds, Email: info@intechopen.com, Website: http://www.intechopen.com/ Phone: +385 51 686 165, Fax: +385 51 686 166, Janeza Trdine 9, 51000 Rijeka, Croatia, 2012, pp. 255-272.
? Silymarin Flavonolignans: Structure-Activity Relationship and Biosynthesis. Sameh AbouZid, Osama M. Ahmed. In "Studies in Natural Products Chemistry", vol. 40, edited by Atta-ur-Rahman, 2013, volume 40, pp. 469-484.
BOOKLET
? "Beni-Suef Wild Plants of Medicinal Importance". Edited by: Sameh AbouZid. Distributed by: The Anglo-Egyptian Bookshop, Deposit number 21278, I.S.B.N. 978-977-05-2864-8, 2014.
SCIENTIFIC MEETINGS, Conferences & Workshops
1. Phytochemical Studies on Ambrosia maritima Hairy Root Culture. Sameh AbouZid and Yutaka Orihara. Annual meeting of the Pharmaceutical Society of Japan. Nippon Yakugakkai Nenkai Koen Yoshishu 2002, 122:151 (poster).
2. Biosynthetic Studies on Ambrosia maritima Hairy Root Culture. Sameh AbouZid and Yutaka Orihara. Annual meeting of the Pharmaceutical Society of Japan. Nippon Yakugakkai Nenkai Koen Yoshishu 2003, 123:148 (poster).
3. Biosynthesis of Polyacetylenes in hairy roots of Ambrosia maritima. Sameh AbouZid and Yutaka Orihara. Annual meeting of the Japanese Society of Plant Cell and Molecular Biology. 2003 (oral).
Sameh AbouZid C.V. 8
4. Studies on polyacetylenes biosynthesis in Ambrosia maritima hairy roots. The 2nd international conference of the Pharmaceutical and Drug Industries Division. National Research Centre, Egypt 2005 (oral).
5. Phytochemical Studies on Plants Growing in Beni-Sueif Governorate, Egypt. Sumer Ezeldin, Inas Ibrahim, Haytham Wahba, Marwa Hassan, Mohammed Zaki and Sameh AbouZid. The 30th International Conference of Pharmaceutical Science. The Egyptian Pharmaceutical Society, p. 43 – Egypt 2006 (poster).
6. Molluscicidal activity of polyacetylenes isolated from transformed roots of Ambrosia maritima. The 2nd Annual Scientific Conference of the Egyptian Society of Environmental Toxicology, Egypt 2006 (oral).
7. Elicitation of flavonoids in red onion (Allium cepa) by mechanical stress. Sameh AbouZid and Ahmed Atiah. The 3rd International Conference of Pharmaceutical and Drug Industries Division, National Research Centre, Egypt 2007 (oral).
8. Steroidal glycoalkaloids from the berries of Solanum distichum. Sameh AbouZid, Nermeen Fawzy, Nebal Darwish, Yutaka Orihara. The 3rd Annual Scientific Conference of the Egyptian Society of Environmental Toxicology, Egypt 2007 (oral).
9. Withaferin A Production by Root Cultures of Withania coagulans. Sameh AbouZid, Asma Nasib, Seif Khan, Javed Qureshi, Mohamed Iqbal Choudhary. Abstract Book of The Fourth International Conference of Pharmaceutical and Drug Industries Division, National Research Centre, Egypt 2009 (poster).
10. Antioxidant and antiviral activities of plants growing in Beni-Sueif governorate, Egypt. Sameh AbouZid and Yutaka Orihara. Abstract Book of The First EuroMed Symposium “Plant Natural Products from Biodiversity to Bioindustry” , National Research Centre, Egypt 2009 (poster).
11. The 238th American Chemical Society (ACS) National Meeting and Exposition, Agricultural and Food Chemistry Division Symposium entitled: “Bioactives: New Production Technologies” August 16-20, 2009; Washington, DC, US (Oral).
12. In Vitro Production of Flavolignans from Silybum marianum. El-fishway AM, Sokkar N, AbouZid S, El-wekeel AH. Assiut University Seventh International Pharmaceutical Science Conference, Assiut, Egypt; March 17 & 18, 2010 (poster).
13. Phytochemical studies on isoflavonoids isolated from the bark and leaves of Dalbergia paniculata. Ahmed Attia Seida, Sameh AbouZid and Enas Ebrahim. Assiut University Seventh International Pharmaceutical Science Conference, Assiut, Egypt; March 17 & 18, 2010 (poster).
14. Medicinal Plants and Their Therapeutic Effects. Symposium, Faculty of Pharmacy – Beni-Suef University 7-8th April 2010 (Organizer).
15. Recent Advances in Pharmaceutical Research. Symposium, Faculty of Pharmacy - Beni-Suef University 12th January 2011 (Listener).
16. Research-Industry Day, from Lab to Reality. Bibliotheca Alexandrina, Alexandria, Egypt; July 28 2011 (Listener).
17. International Symposium on Natural Product Chemistry, September 22-35, 2012; Karachi, Pakistan (Oral).
Sameh AbouZid C.V. 9
18. Plant Biotechnology Classical & New trends Symposium, 5 December 2013, National Research Centre, Cairo, Egypt (Listener).
19. The 15th symposium of the Natural Products Chemistry Research Network for Eastern and Central Africa (NAPRECA), Khartoum - Sudan, 7-10 December 2013 (Poster).
20. International symposium on "Recent advances in Chemistry and Biology of Natural Product", Beni-Suef, Egypt, 3-4 August 2014 (Event Coordinator).
21. Symposium titled "Biotechnology for the Emerging Economies:, Beni-Suef, Egypt, 7 April 2015 (Speaker).
22. Workshop titled "Modern Approach in Publishing Ethics", March 29, 2016, Academy of Scientific Research and Technology, Egypt.
23. Workshop titled "Improving Journal Peer Review", May 9, 2016, Academy of Scientific Research and Technology, Egypt.
24. Workshop titled "Criteria of Selection of Journals for Web of Science Indexing:, October 13, 2016, Academy of Scientific Research and Technology, Egypt.
INVITED LECTURES
? Hairy roots technique and secondary metabolites. Training course on secondary pharmaceutical metabolites, Agricultural Research Centre, Egypt, July 2009.
? American Chemical Society (ACS) Agricultural and Food Chemistry Division, symposium entitled: “Bioactives: New Production Technologies” The 238th ACS National Meeting and Exposition, August 16-20, 2009; Washington, DC, US.
? In vitro production of flavonolignans from Silybum marianum. 13th International Symposium on Natural Product Chemistry, September 22-35, 2012; Karachi, Pakistan.
? American Chemical Society (ACS) Agricultural and Food Chemistry Division, Use of Nuclear Magnetic Resonance Spectroscopy in Quality Control of Fennel Essential Oil. ACS AGFD - ACS ICSCT Symposium, March 4-5, 2014, Thailand.
? Yield Improvement Strategies for Production of Secondary Metabolites in Plant Tissue Culture. Second International Course for Plant Tissue Culture Applications, May 18-22, 2014, Agriculture Research Center, Egypt.

Master Title

Phytochemical studies on tissue culture of Ambrosia maritima: an Egyptian medicinal plant for renal diseases

Master Abstract

Successful induction and maintenance of hairy root culture of Ambrosia maritima, a system to study the biosynthetic machinery of this species on the organ level. Induction and maintenance of the static and cell cultures were also achieved. Optimization of the growth conditions, concerning the growth regulators and ammonium ion for the static culture were carried out. Still we have the following questions not answered yet: are any of the established cultures able to produce sesquiterpene lactones, the biologically active components of this species. Whether or not, the hairy root culture can be successfully elicited to produce polyacetylene phytoalexins, previously isolated from Ambrosia artemisifolia. What are the optimum media composition and other culture conditions for high production of the biologically active compounds as well as high growth rate.

PHD Title

Studies on the polyacetylenes from the tissue culture of Ambrosia maritima, an Egyptian medicinal plant

PHD Abstract

Many species of the family Asteraceae, the largest family of the higher plants, is known to produce a wide variety of structural types of polyacetylenes (PA). These compounds possess significant antibiotic, antiviral and nematicidal activities. In this research work, we have developed a transformed root culture and a callus culture systems from the plant Ambrosia maritima, a weed growing on the Nile banks. We have cleared some of the important points in the pathway of biosynthesis of PA produced by these systems. Additionally, we have discovered a new biological activity for PA as molluscicidal agents. 1- Establishment of the plant tissue culture system For the root culture 3 lines were established from the sterile plant infected with Agrobacterium rhizogenes ATCC15834. These lines, continuously subcultured on Murashige and Skoog (MS) agar basal media at 25 oC in the dark, were inoculated in MS liquid media and cultured on 50 rpm in the dark. Growth time curve showed significant difference between the three lines. Callus culture was initiated by cutting the sterile plant material and putting it on MS agar media contianing 2,4-dicholorophenoxyacetic acid. The callus was maintained on MS agar media containing naphthalene acetic acid (1 ppm) and kintein (0.1 ppm) (NK media). Cell suspension culture was initiated by inoculating callus tissues into NK liquid media and was cultured on 100 rpm in the dark. 2- Biosynthetic studies A- Determination of the optimum yield conditions An elicitation strategy was applied to maximize the yield of PA. The elicitor used was methyl jasmonate (MeJ), the common signal molecule in plant stress and development. Optimum conditions were obtained by eliciting the root tissues for 72 h in the early log phase of growth with 40µM MeJ. Metabolic profiling for the biomass obtianed after elicitation showed thiarubrine A (1), its epoxide (2) and diol (3) as well as its precursor pentaynene (4) (Fig. 1) as the main PA produced by the hairy root. Thiophene A (5) and its diol (6) (Fig. 1), the photodegradative products of their corresponding thiarubrines, were isolated from hairy root cultured under continuous light illumination. When the cell suspension culture was challenged with MeJ, only 4 could be found. B- Structure elucidation and assignment of the 13C-NMR signals The structure elucidation and assignment of the each carbon signal to its corresponding position was based on MS, 1D- and 2D-NMR spectroscopic analysis. Fig. 1. Chemical structure of the isolated polyacetylenes C- Feeding experiments Our biosynthetic study for 1 and 5, using [1-13C]-, [2-13C]- and [1,2-13C2]- acetates suggested either anabolic or catabolic pathways. The key steps leading from acetates to 4 are supposed to proceed through unsaturated fatty acid, based mainly on co-occurrence and structural similarity. However, not much is known about the order of these reactions or the specificity of the enzyme involved. When we studied the global effects of MeJ on the root tissues (Fig. 2), we found an increase in linoleic acid (LA) (peak at 120 h) along with that obsverved for 1 and its precursor 4 (peak at 72 h). Fig. 2. Effects of MeJ on metabolite biosynthesis and LOX activity Complementary to the above results, incubation of root cultures with linoleic acid-13C18 resulted in the production of labelled 1. The pattern of enrichment suggested a biosynthetic pathway by chain length reduction and desaturation of the fatty acid precursor. The involvement of LA in polyacetylene biosynthesis suggested a pathway that may include lipoxygenase (LOX), an enzyme which catalyzes the hydroperoxidation of polyunsaturated fatty acids. LOX activity was found to peak 24 h after elicitor addition and before the increase in polyacetylene production (Fig. 2). N-propyl gallate, a LOX inhibitor, had significantly reduced the polyacetylene production at 100µM. In conclusion, our results suggest a catabolic pathway for compound 1 biosynthesis from LA with a possible involvement of LOX at an early stage of the pathway (Fig. 3). Fig. 3 Hypothesis for PA biosynthesis in the root culture of A. maritima 3- The effect of PA on the viability of Biomphalaria glabrata, schistosome intermediate host Schistosomiasis is a parasitic disease representing a major health risk in the rural areas 74 tropical developing countries. It is estimated that 200 million people are infected with this parasite. One of the effective methods to control of the disease is to control the intermediate host (freshwater snail = mollusc) using molluscicidal agents. Those agents of plant origin are less toxic as they are part of the local ecosystem. The molluscicidal activities of both A. maritima transformed root extract and individual PA, were studied against schistosome intermediate host Biomphalaria glabrata. The chloroform extract of the MeJ treated root was effective in killing 100% of the snails at concentration of 50 ppm after 5 days of exposure. While compound 1 showed strong activity (killing 93% of the snails at a concentration of 5 ppm with a LC50 of 2.64 ppm), when compared to that of ambrosin, a sesquiterpene lactone that long considered as the active molluscicidal agent from the aerial parts of the plant. When the other PA were tested, the order of activity was as such 4 < 1 < 2 < 3. Compound 3, which showed the highest activity, was the only form secreted to the media from these PA. As such, we clear the ecological picture for the role of these metabolites. The plant root can synthesize them from primary metabolites, then increase their polarity by epoxide and diol formation. The diol is then secreted to the media to act as an allelopathic agent in the surrounding environment.