Basic Informations

C.V

CURRICULUM VITAE

PERSONAL DETAILS

Title: Dr	DOB: 16/4/1971
First Name: Reem	Sex: Female
Surname : Hashem Ahmed	Nationality: Egyptian
Position: Postdoctoral Researcher
Address:  Centre for Skin Sciences                  School of Life Sciences                   University of Bradford                  BD71DP	Home address: Flat 42H, Byron Halls                            Byron Street                            Bradford                            BD3 0AR
Telephone no:   +44 (0)1274 235 917	Mobile: +44 (0)7918445550
Email: R.Ahmed29@bradford.ac.uk	Reem.samy@pharm.bsu.edu.eg

PROFFESIONAL QUALIFICATION/MEMBERSHIP

2/02- 6/05 PhD in Biochemistry, Faculty of Pharmacy, Zagazig University, Egypt

3/98- 12/01 MSc in Biochemistry, Faculty of Pharmacy, Zagazig University, Egypt

9/88- 5/93 B.Sc in Pharmaceutical Science, Faculty of Pharmacy, Zagazig University, Egypt

Membership of the Egyptian Society of Biochemistry & Molecular Biology (ESBMB).

Membership of the Egyptian Society of Clinical Biochemistry & Molecular Biology (ESCC).

DETAILS OF CURRENT  POST

Postdoctoral Researcher (05/2014-10/2016)

Employer’ name and address: Centre for Skin Science, School of Life Science, Bradford University

Brief description of duties:

• To conduct research, develop and deliver the research objectives of the project that has been agreed with the academic PI.
• To accurately record data and maintain records of research activity and progress, compile data spreadsheets, figures and reports, and perform statistical evaluation of data sets.
• To prepare SOPs and COSHH forms for all the lab procedures related to the project.
• To write up work for publication in peer-reviewed journals.
• To liaise with and build internal contacts and participate in internal networks (journal clubs, interest groups) for the exchange of information

DETAILS OF PREVIOUS  POST

Position:  Associate Professor of Biochemistry since (02/2011)                                                                                                                                           

Employers’ name and address: Biochemistry Department, Faculty of Pharmacy, Beni-Sueif                                                                                                                                     University.

Telephone of work: 0020822317958

Fax: 0020822317958

Brief description of duties:

• Teaching of Principals of Biochemistry and Molecular Biology, Metabolism of Macromolecules Nutrition and Clinical Chemistry and Biotechnology for undergraduates.                                                                                                                        
• Supervision on the practical sections of undergraduates.
• Preparation of the theoretical notebooks of different courses of the undergraduates
• Preparation of, assignments, quizzes, and theoretical exams of undergraduates.
• Supervision on practical exams and oral exams 
• Head control of the level one student (collecting and announcement of results of oral, practical, assignments, and written exams)
• Academic advisor for undergraduates
• Supervision on assignments in the summer training of the undergraduates.

• External oral examiner for different Biochemistry Departments in other Universities
• Supervision on Master and Doctorate Thesis of Postgraduates

• Developing, designing and revision of curriculum offered based on the course specification.
• Preparation of the courses reports of each final term
• Conducting research work , writing, and interpreting the obtained results 
• Publishing in peer-reviewed journals, and presenting at conferences or symposiums.
• Teaching of postgraduates (Hormones, Molecular Biology, Principles of biochemistry, Metabolism of Macromolecules, lab techniques) and preparation of theoretical exams.

Position:  Head Department of Biochemistry since (09/2011-09/2013)                                                                                                                                           

Employers’ name and address: Biochemistry Department Faculty of Pharmacy, Beni-Sueif                                                                                                                                     University.

Telephone of work: 0020822317958

Fax: 0020822317958

Brief Duties related to Head of Biochemistry Department                                                                

• Planning and contributing to ongoing research program in of the Department as well as in collaboration with other Universities and supervising the master and Ph.D. degrees
• Manage all staff within the education and research job family and other staff within the Department as delegated by the Dean, including performance management, staff development and performance review reports.

• Contribution to the  strategic development of the Faculty and University

• Participate actively as a member of the university community: on administrative committees, search committees, in university governance activities, and student advising.

Position:   Vice Dean of Social Affairs and Environmental Development since (10/2012 -09/2013)                                                                                                                                          

Employers’ name and address: Faculty of Pharmacy, Beni Suef University.

Telephone of work: 0020822317958

Fax: 0020822317958

Duties related to Vice Dean of Social Affairs and Environmental Development                                                                  

• Preparation of a study to determine the types of societal problems
• Develop a plan of activities during the year based on the needs of the community (Seminars, conferences and campaigns)
• Contribute to the provision of  health and safety and secure work environment
• Participation in the faculty increase sources of income and financial resources through the stakeholders and the services provided by the special units.

PREVIOUS EMPLOYMENT RECORD

EDUCATION AND QUALIFICATIONS

WORKSHOPS FOR QUALITY ASSURANCE:

 

• Course description A. 2006. Cairo Universty. Egypt.
• Course description B. 2006 Ain Shams University Guest House, Cairo, Egypt.
• Program description. 2006. Faculty of Pharmacy, Beni-Suef University.
• Accreditation and Research. July 2010. National Authority for Quality Assurance & Accreditation of Education-Egypt (NAQAAEA).

SHORT COURSES ATTENDED

1. Data Analysis using SPSS 2. June 1, 2015.  L24 Richmond Building. Bradford University  
2. Data Analysis using SPSS 1. June 1, 2015.  J31 Richmond Building. Bradford University  
3. Diversity in the workplace e-module.  May 15, 2015.  Bradford University  
4. Winning Grant Funding.  April 21, 2015.  D3 Richmond Building, Bradford University
5. Research Ethics Approval Process. Nov 5, 2014.  C7, Richmond Building, Bradford University
6. HTA (Human Tissue Authority). Nov 11, 2014.  Richmond Building, Bradford University
7. Laser Safety Training course. November 12, 2014.  Phoenix SA0.08, Bradford University
8. Curriculum Design. November 28-30; 2006.  Beni-Suef University
9. University Law and Regulation. November 21-23; 2006.  Beni-Suef University
10. Scientific Research Management. November 14-16; 2006.  Beni-Suef University
11. Organization of meeting and conferences. October 4-6; 2010. Zagazig university
12. Communication skills. October 11-13; 2010. Zagazig university
13. International and local competing research projects. October14-16;2010.Zagazig university
14. Credit hours. October 21-23 ;2010 Beni-Suef University

SCIENTIFIC ACTIVITIES

• Reviewer for

1. FEBS Journal
2. Cytokine
3. Molecular Biology Reports
4. Journal of  Physiology and Pathophysiology
5. Medicine and Medical Sciences

PUBLICATIONS                                

Reem Hashem Ahmed, Sonia Manaf, Haleema Sajid, Asram Munir, Stephen K. Sikkink, Rachael Sedman- Sutherland, David A, Desmond J. Tobin. Fenton.  Evidence for alopecia areata and celiac disease cross-reactive epitopes expressed by anagen hair follicle inner root sheath - implications for alopecia areata autoantigen(s) discovery.  9th World Congress for Hair Research November 18-21, 2015

Hashem RM, Hassanin KM, Rashed LA, Mahmoud MO, Hassan MG. Effect of silibinin and vitamin E on the ASK1-p38 MAPK pathway in D-galactosamine/lipopolysaccharide induced hepatotoxicity.

Exp Biol Med (Maywood). 2016 Jun;241(11):1250-7

Hashem RM*, Mohamed RH, Abo-El-matty DM, Soliman HM. Effect of curcumin on TNFR2 and TRAF2 in unilateral ureteral obstruction in rats. Nutrition. 2016 Apr;32(4):478-85.

                                                       

Hussein RM, Hashem RM, Rashed LA.  Evaluation of the amyloid beta-GFP fusion protein as a model of amyloid beta peptides-mediated aggregation: A study of DNAJB6 chaperone.  Front Mol Neurosci. 2015 Jul 27;8:40. doi: 10.3389/fnmol.2015.00040

Hashem RM.*, Rashd LA, Hashem KS, Soliman HM. Cerium oxide nanoparticles alleviate oxidative stress and decreases Nrf-2/HO-1 in D -GALN/LPS induced hepatotoxicity. Biomedicine & Pharmacotherapy, Volume 73, July 2015, Pages 80-86

 Månsson C, Arosio P, Hussein R, Kampinga HH, Hashem RM, Boelens WC, Dobson CM, Knowles TP, Linse S, Emanuelsson C. Interaction of the molecular chaperone DNAJB6 with growing amyloid-beta 42 (Aβ42) aggregates leads to sub-stoichiometric inhibition of amyloid formation. J Biol Chem. 2014 Nov 7;289(45):31066-76.

Motawi TK, Hamed MA, Hashem RM, Shabana MH, Ahmed YR. Protective and therapeutic effects of Argyreia speciosa against ethanol-induced gastric ulcer in rats. Z Naturforsch C. 2012 Jan-Feb;67(1-2):47-57.

Rashed LA, Hashem RM*, Soliman HM. Oxytocin inhibits NADPH oxidase and P38 MAPK in cisplatin-induced nephrotoxicity. Biomed Pharmacother. 2011 Oct;65(7):474-80.

Motawi TK, Hamed MA, Shabana MH, Hashem RM, Aboul Naser AF. Zingiber officinale acts as a nutraceutical agent against liver fibrosis. Nutr Metab (Lond). 2011 Jun 20;8:40.

Abd El-Aziz TA, Mohamed RH, Hashem RM. Association of lipoprotein lipase and apolipoprotein C-III genes polymorphism with acute myocardial infarction in diabetic patients. Mol Cell Biochem. 2011 Aug;354(1-2):141-50.

Motawei TM, Hashem RM*, Rashed LA, EL-Razek SM. Comparative study between the effect of the PPAR-α ligands, fenofibrate and n-3 PUFA on activation of AMPK-α 1 in high fat fed rats. J Pharm Pharmacol. 2009 Oct;61(10):1339-46

Hashem RM*, Mahmoud MF, EL-Moselhy MA, Soliman HM. Interleukin-10 to tumor necrosis factor-alpha ratio is a predictive biomarker in nonalcoholic fatty liver disease: interleukin-10 to tumor necrosis factor-alpha ratio in steatohepatitis. Eur J Gastroenterol Hepatol. 2008 Oct;20(10):995-1001.

El-Seweidy MM, Hashem RM*, Abo-El-matty DM, Mohamed RH. Frequent inadequate supply of micronutrients in fast food induces oxidative stress and inflammation in testicular tissues of weanling rats. J Pharm Pharmacol. 2008 Sep;60(9):1237-42.

Hashem RM*, Soliman HM, Shaapan SF. Turmeric based diet can delay apoptosis without modulating NF-kB in unilateral ureteral obstruction in rats. J Pharm Pharmacol. 2008 Jan;60(1):83-9.

El Seweidy MM, El-Swefy SE, Abdallah FR, Hashem RM. Dietary fatty acid unsaturation levels, lipoprotein oxidation and circulating chemokine in experimentally induced atherosclerotic rats. J Pharm Pharmacol. 2005 Nov;57(11):1467-74.                                                                                       

Master Title

“Relation of Certain Cellular Mediators to Glycation and Oxidative Stress in Experimental Diabetes Mellitus

Master Abstract

Until now the relation between advanced glycation end products (AGEs) and vascular lesion is still controversial. However, the interaction of the former with a receptor triggers the synthesis of cytokines particularly interleukin 1- beta(IL-1 beta) and tumour necrosis factor- alpha(TNFalpha ). Subsequent release of nitric oxide (NO) may in turn induce certain damage to beta cell islets. Several arguments indicated that AGEs and reactive oxygen intermediates (ROIs) could alter the function of the vessel wall. Therefore, this study was undertaken to investigate the effectiveness of aminoguanidine, AG (inhibitor of AGE formation) joined with omega -3-fatty acids, omega 3FAs (anti-inflammatory immunosuppressive drug) in STZ diabetic rats. Diabetes was induced in 48 female albino Wistar rats by a single intraperitoneal (i.p.) injection of streptozotocin (STZ, 50 mg kg (-1)). Diabetic animals were treated with AG (50 mg kg(-1) ) and/or omega 3FAs (12 mg kg (-1)) daily and orally for 4 weeks. Groups of age matched diabetic rats ( n= 10) and healthy animals ( n= 10) served as positive and negative controls. At the end of the study, plasma glucose, fructosamine, total cholesterol (TC), high density lipoprotein cholesterol (HDLC), low density lipoprotein cholesterol (LDLC), the susceptibility of LDL to copper-catalysed oxidation, catalase activity, NO, IL-1 beta, TNF alpha were measured. Histopathological assessment of pancreatic slices were also determined. Diabetes remarkably increased plasma glucose, fructosamine and dyslipidaemia (increased TC, LDLC and decreased HDLC). Oxidative markers like oxidative susceptibility of LDL, catalase activity and NO levels were greatly enhanced. Finally, it increased the synthesis and release of cytokine (IL-1beta and TNF alpha). Treatment of diabetic rats with AG and omega 3FAs markedly reduced the above mentioned parameters. Combined form therapy has a better effect regarding oxidative cell markers, specifically NO level. Finally, omega 3FAs coadministration with AG nearly restored the atrophy of islets of Langerhan's and the peripheral lymphocytic infiltration compared to diabetic and AG treated groups. In conclusion, there is a direct correlation between glycation, oxidative stress and cytokine production with increased propensity of microvascular disorder in STZ diabetic rats. omega 3FA administration with AGE receptor blocker may represent a possible avenue of research for therapeutics directed for alleviating the complication associated with diabetes.

PHD Title

Role of Certain New Factors in Modulation And Treatment of Hyperlipidemia Associated with Hyperglycemia in Experimental Atherosclerosis

PHD Abstract

Summary and Conclusion Present work aimed mainly to: 1. Study some factors dealing with induced hyperlipemia and in experimental atherosclerosis in experimental rats. This is done through long term supplementation of diet enriched with saturated fats and cholesterol then followed by alloxan administration. 2. Reflection of this on certain changes recorded for selected biochemical parameters in blood and aortic tissues. This was followed by histopathological studies for aorta using different stains. The study included two parts. First part: deal with the effect of certain drugs focused on: 1- Angiotensin converting enzyme inhibitor (ACEI) 2- Angiotensin type 1 receptor blocker 3- Role of natural antioxidant represented by green tea extract, catechin either alone or in combination with drugs (1&2). Second part: effect of dietary components having various degrees of unsaturated fatty acids. Biochemical markers selected were: Serum: Glucose, lipid profile, NO, CRP, chemokines expression (MCP-1 and RANTES). Plasma: susceptibility of non HDL-C to oxidation. Aorta tissues: Glutathione (GSH), super oxide dismutase (SOD) enzymes. Histological study: using different stains A- Hematoxylin and eosin: to illustrate any changes like cellular infiltration, foam cells, and thickening changes in arterial walls. B- Alcian blue: to study glycosaminoglycans distribution in vessel wall. C- Masson trichrome: to study collagen distribution as related to smooth muscle cells. D- Modified Taenzer-unna Orcein method: to study elasticity and corrugation of fibers. Experimental design: The study was performed in two parts The first part: Rats were fed chow diet supplemented with 3% saturated fatty acid 1%cholestrol and 0.5% cholic acid, and 0.01% thiouracil for four months. Subsequently received alloxan; 150 mg/ kg, IP(1 dose), after. Acclimatization, rats were categorized as follow: Group (1): Hypercholesterolemic hyperglycemic rats received enalapril Group (2): Hypercholesterolemic hyperglycemic rats received losartan Group (3): Hypercholesterolemi hyperglycemic rats received catechin Group (4): Hypercholesterolemic hyperglycemic rats received enalapril and losartan Group (5): Hypercholesterolemic hyperglycemic rats received enalapril, losartan and atechin Treatment regimen was continued for 4 and 8 weeks Results 1- Rats received hypercholesterolemic diet followed by alloxan injection demonstrated significant increase in glucose, lipid profile (TC, TAG, LDL), susceptibility of non-HDL-C to oxidation, atherogenic indexes (TC/HDL, LDL/HDL), inflammatory markers (CRP), chemokine expression. Moreover significant decrease was demonstrated in NO and aortic antioxidant content (GSH and SOD). 2- Histopathological examination revealed massive cellular infiltration, foam cell formation, and marked distribution of glycosaminoglycans allover the aortic layers. Increased collagen synthesis and degradation joined with loss of elastic fibers corrugation was also observed. 1-ACEI (Enalapril) Enalapril administration induced significant decrease in serum glucose levels (after 8 weeks). Significant decrease in TC, TG, LDL,atherogenic indexes, and susceptibility of non-HDL-C to oxidation was observed, while HDL-C demonstrated non significant effect. Significant increase in NO, aortic GSH and SOD was also achieved. MCP-1 and RANTES revealed significant decrease in comparison to control group. Histopathological examination illustrated moderate distribution of foam cells, cellular infiltrate, and glycosaminoglycans. Collagen fibers amount and elasticity demonstrated moderate improvement. 2 –AT1RA (Losartan): Losartan administration induced significant decrease in serum glucose (after 8 weeks). Hyperlipemia and atherogenic indexes (TC/HDL, LDL/HDL) show non significant change. While 8 weeks of continual administration significantly decreased susceptibility of non HDL–C to oxidation, MCP-1, and RANTES. However it increased NO level. CRP, aortic GSH and SOD illustrated non significant change. Histopathological examination showed massive infiltration of intima and media with foam cells, cellular infiltrate and glycosaminoglycans. Massive increase in collagen fibers and loss of elastic fibers corrugation was also observed. 3- Antioxidant (catechin): catechin administration significantly decreased serum glucose, TC, TAG, LDL and the atherogenic ratios (LDL/HDL and TC/HDL) meanwhile HDL-C recorded non significant change. Susceptibility of non HDL oxidation, CRP, MCP-1 and RANTES showed significant decrease. However aortic GSH and SOD content recorded significant increase in comparison to control group. Histopathological examination showed moderate cellular infiltration in intima, media and adventitia with marked improvement in foam cells amount, joined with minimal amount of glycosaminoglycans. Minimal amount of collagen fibers and marked improvement of fibers elasticity and corrugation were also observed. 4–Combination of enlalapril and losartan: Combined administration of these drugs induced significant decrease in serum glucose (after 8 weeks), TC, TAG, LDL, atherogenic indexes (TC/HDL and LDL/HDL), CRP, MCP-1, and RANTES. NO and aortic GSH and SOD content demonstrated significant increase in comparison to control group. Histopathological examination illustrated moderate improvement in cellular infiltration and foam cell formation. glycosaminoglycans distribution was moderate in intima, media and adventitia. Relative improvement in collagen appearance with moderate loss of corrugation of elastic fibers was also recoded. 5- Combination of enalapril, losartan and catechin: Tritherapy administration induced significant decrease in blood glucose, TC, TAG, LDL, susceptibility of non HDL-C to oxidation, atherogenic indexes (TC/HDL and LDL/HDL), CRP, MCP-1 and RANTES. NO level, aortic GSH and SOD demonstrated significant increase in comparison to control group. Histological examination showed marked improvement in both of cellular infilteration and foam cell formation. Minimal amount of glycosaminoglycans was observed in intima, media and adventitia. Collagen amount was decreased greatly and elastic fibers appeared weavy with marked improvement in corrugation. The second Part: Animals received chow diet consisted of 21% saturated fat enriched with 1% cholesterol, 0.5% cholic acid, and 0.01% thiouracil for four monthes. Atherogenic rats were randomly divided into three experimental groups. First group received chow diet supplemented with 10% v/w OO Second group received chow diet enriched with 10 % v/w of SO Last group received chow diet enriched with 10 % v/w FO This dietary regimen was continued for 8 weeks. Results: Atherogenic diet induced dyslpidemia state represented by elevated TC, LDLC, TAG and marked increase in oxidative stress markers. Increased LDL oxidation susceptibility and decreased aortic antioxidant enzymes (SOD and GSH) are examples. CRP, MCP-1, and RANTES showed significant increase and greatly exaggerated in comparison with baseline data. Histopathological examination of the atherosclerotic aorta showed thick intima with thick and irregular endothelium subendothelial basal lamina (SBL), multiple cellular infiltrations and foam cells in addition to white longitudinal streaks as compared to normal aorta. Also there was marked increase in the positively stained area of glycosaminoglycan in intima, media and adventitia compared to control rats. Massive increase in amount of collagen combined with loss of corrugation of elastic fibers was also observed. 1- Virgin olive oil (OO) : Rats received OO illustrated moderate decrease in lipogram pattern, atherogenic index, inflammatory marker (CRP) and chemokine expression (MCP-1 and RANTES). Decreased OX-LDL and increased antioxidant enzymes (SOD and GSH) in comparison to atherogenic control group was also observed. Histopathological examination showed relatively thin intima with thin endothelium. Reduced infiltration, vacuolations, white longitudinal streaks of intima and media with minimal infiltration with glycosaminoglycan. Significant amelioration of collagen amount with obvious corrugation increase. 2- Sunflower oil (SO): Rats received SO enriched diet illustrated non significant change in the lipogram pattern and atherogenic index after 4 and 8 weeks. Antioxidant effect was mild. This was manifested by decreased lipoprotein susceptibility to oxidation and aortic GSH increase after 4 and 8 weeks. Aortic SOD recorded significant increase after 8 weeks only. MCP-1 Significantly decreased after 4 and 8 weeks additionally RANTES (after 8 weeks) in comparison to atherogenic diet group. Histopathological examination showed marked cellular infiltration and foam cell formation in intima and media joined with marked increase in glycosaminoglycans amount. Obvious amount of collagen and loss of corrugation of elastic fibers was still evident. 3- Omega 3 fatty acid (FO): Supplementation with FO significantly reduced lipogram pattern and atherogenic indexes. CRP, MCP-1, and RANTES recorded significant decrease. Moderate decrease in susceptibility of lipoprotein to oxidation joined with aortic GSH and SOD increase were also observed after 4 and 8 weeks. Histopathological examination illustrated few cellular infiltrate and few white longitudinal streaks are observed in media. Additionally relative reduction in glycosaminoglycan distribution as compared to atherosclerotic group. Moderate collagen amount and corrugation of elastic fibers was also observed in the aortic stained rings. Conclusion - Atherosclerosis disease represents the net results of certain biological reactions in the blood and effectively pronounced later in the arterial walls. This does not neglect the early changes that probably occur between the two (blood and tissue). - Hyperlipemic state was induced in experimental rats though long intake of diet supplemented with cholesterol and saturated fatty acids. - Rennin angiotensin system may play vital role in induction phase of atherosclerosis. This may be through significant increase in chemokines (MCP-1 and RANTES), oxidative stress, and antioxidant enzymes decrease. - Inflammatory markers, chemokines from one side showed positive correlation with hyperlipemia, oxidative stress markers and from the other side with antioxidant enzymes. - Enalapril and losartan effects were focused clearly on MCP-1, RANRES and CRP joined with minimal effects on lipogram pattern and oxidative stress markers, this may leave an impression that angiotensin pathway was not alone in sequences leading to induced atherosclerosis. Therefore it may require the collaboration of other factors. - Catechin (green tea extract) appeared as hypolypemic antioxidants, decreased the susceptibly of non HDL-C to oxidatiation represents an example takes in consideration that it have immunogenic and chemoattractant properties (MCP-1 and RANTES) - Combined administration of enalapril, losartan, catechin induced certain improvement in biochemical markers additionally aortic tissues. - Catechin being effective and known hypolipemic agents in turn, its inclusion in such combination was additive as potential treatment. This was manifested and viewed through decreased foam cells (catechin effect), cellular infiltration degree (enalapril & losartan) and collagen amount joined with marked improvements in elastic fibers corrugation. - Diet containing higher degree of unsaturated fatty acids was greatly effective. Marked improvement produced in biochemical parameters studied (lipogram pattern, oxidative stress, inflammatory makers and chemokines expression in turn pattern of cellular aorta are confirmative. - Although omega 3 fatty acids have hypolipemic, antioxidant and anti-inflammatory properties, olive oil appeared more effective. The later have antioxidant (polyphenolic content) and moderate anti-inflammatory properties. This was clearly evident, reflected through certain improvement produced in cellular pattern of the aorta (decreased atherosclerotic lesions) and comparably more than omega 3 fatty acids and lastly sunflower oil.