Fatma Mostafa Mohamed Korni

lectuere

Basic Informations

C.V

1. Personal details

Name: Fatma Mostafa Mohamed Korni

Date of birth: 8-11­ 1981                                                     Place of birth: Beni­Suef

Sex: Female                                                                           Nationality: Egyptian

Marital status: Married                                                       Children: 2

Address in Egypt: 6th of October, Giza.                              Telephone/ Fax: 0 020822327982.

 Mobile: 01100921799-01001154552                                 E­mail: naglaamostafa1@yahoo.com

Orcid ID: 0000-0002-1236-400X

2. Scientific degrees

­ Bachelor of Veterinary Medicine Sciences, (2004); Faculty of Veterinary Medicine, Cairo University, Beni–Suef  branch, Egypt.

­ Master of Science in Fish Diseases and Management (2008); thesis title: Lernaeosis and its control in hatchery reared cyprinids­ Faculty of Veterinary Medicine, Cairo University, Beni-Suef branch, Egypt.

­Ph.D. in Fish Diseases and Management (2012); thesis title: Edwardsiellosis in Some Freshwater Fishes, Beni­Suef University, Egypt, 2012.

3. PROFESSIONAL EMPLOYMENT

*Demonstrator of fish diseases and management from 2005­2007, Beni­Suef University, Beni­Suef, Egypt (teaching the practical courses to undergraduate students).

* Assistant lecturer in fish diseases and management from 2008­2011, Beni­ Suef University, Beni­Suef, Egypt (teaching the practical courses to undergraduate students).

* Lecturer in fish diseases and management from 2012­till now, Beni­Suef University, Beni­Suef, Egypt (teaching the practical & theoretical courses to undergraduate students).

4. Teaching experience and research interest

a- Teaching undergraduates students the practical and theoretic courses.

b- Postgraduate studies

5. Thesis supervision

a. Master Degree:

Phenotypic and genotypic characterization of Aeromonas species recovered from different hosts.

b. Ph. D. Degree:

Nano technological approach in prevention and control of some fish bacterial diseases.

6. Reviewer in Turkish Journal of Fisheries and Aquaculture Research.

Publisher: Central fisheries research institute of Turkey and Japan international cooperation agency (JICA). ISSN. 1303-2712.

7.  Quality assurance activities

Coordinator of students program of fish diseases and management.

 Member of standard credibility and descendants.

8. Projects

-Title: Lernaeosis and its control in hatchery reared cyprinids (2014), Beni-Suef University. Egypt.

- P.I.: Prof. Dr. Manal Adel Ahmed Essa. Professor  of fish diseases and management. Department of fish diseases and management. Beni-Suef University. Egypt.

- Co. P.I. Dr. Fatma Mostafa Mohamed Korni. Lecturer of fish diseases and management. Department of fish diseases and management. Beni-Suef University. Egypt.

-Duration: from 2015-2016.                  -Fund: 40000 L.E.

9. Conferences

#The 7th Global conference of fisheries and aquaculture  research. 17-18/11/2014.

#Hazards facing animal and poultry health. (Challenges and opportunities). 25-29/10/2016

#The 9th  international scientific conference of the faculty of veterinary medicine, Suez Canal university, Ismailia, Egypt. 23-26/11/2016

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10. Voluntarily and obligatory training courses which were passing

1- Quality standards in teaching, from 16-17/7/2017

2- Time and meeting management from 18-19/7/2017

3- Presentation skills from 18-19/7/2017

4- Competitive research projects at 24/7/2017

5- International publishing of research from25-26/7/2017

6- University legal and financial aspects from 25-26/7/2017

 

11. Workshops

1- Aquaculture, principles and practices training program in the period of 26-30/8/2007.

2- Application methods pathobiology field in the period  of 17-20/5/ 2009 (included morphological image analysis, semiquantitative measurements for biomarkers, histochemistry, microdissection, pcr, tissue microarray and comet assay).

 

3- Nanotechnology training course in the period  of 3-5 March- 2015 (included fundamentals of nanoscience, fundamental of Biotechnology,  preparation & characterization of nanomaterials , biosynthesis of Nanoparticles, applications of nanoscience in biotechnology everyday life, medicine, industry, environmental … etc.).

 

12. Training courses

1- ????????? ?????????: ????? ????? ?????? ????? ?????? ?? ???? ???????. 16-9-2014

2- ?????? ????????? ??? ??? ??????? ??????. 13-3-2016

3- -??? ??? ????? ?????? ?????? ??????? ?????? ????? ?????? 2015-2016 ?? ????? ??????? ??????? 2012-2017.?? ???????? ?????? 23-6-2016.

4- ????????? ?????????: ???? ???????? ???????? ?????????. 28-8-2016

5- ??????? ?????? ???? ??????? ??????? ?? ???? ???? ???????. 4-9-2016

6- ?????? ????? ?????? ??????. 18-9-2016

7- ???? ????? ???????? ??????? ???????? ?? ????? ??????. 23-10-2016

8- ?????? ????? ?? ????? ?????? ?????? ???????? ???????. 25-10-2016

9- ????? ????? ???? ??? ???? ??? ??????? ??????. 26-3-2017

10-  ??????? ??????? ??????? ??? ???????? ???????. 14-16 ???? 2017

11 - nano-biosensors: Overview, application and prospects. 9-5-2017

 

12-The Chevening information session. 25-september-2016

 

13.  Computer skills

1­ Knowledge and ability to use most of windows software applications including Microsoft Offices preparation. Screen presentations using PowerPoint, photo shop in addition to statistical analysis using computer statistical programs.

2­ International Computer Driving License (ICDL)

14.Language skills

Toefl

Native Arabic speaker

Very good knowledge of spoken as well as written English

15. List of publications

-Control of Lernaeosis in hatchery reared Cyprinus carpio fingerlings by colophony and its steamed oil. M.A.A. Abd El-Galil, Manal A.A. Essa, Fatma M.M. Korni. Global Aquaculture & Fisheries Research Conference & Exhibition. Cairo, Egypt, October 24-26 (2009) 353-365.

 

 -Diagnosis and safe prevention of Edwardsiellosis in Oreochromis niloticus. Manal A.A. Essa, M.M.A. Hussein, M.A.A. Abd El-Galil, and Fatma M.M. Korni. Global Proc. of The 5th Global Fisheries & Aqua. Research Conf., Egypt, (2012) (5) pp. 147 - 159.

 

-Lernaeosis affecting hatchery reared common carp (Cyprinus carpio) fries and a novel approach for its treatment. Fatma  M. M Korni. Global Journal of Fisheries and Aquaculture Res., (2014).1, (2): pp. 173 -189.

 

- Experimental infection of atypical Aeromonas salmonicida in Nile tilapia Oreochromis niloticus and its treatment with carvacrol and cymene mixture. Fatma Mostafa Mohamed Korni. J. Fish Pathol., (2015).28 (3) : 145-155.

 

- Diagnosis of motile aeromonas septicemia in Catfish, Clarias gariepinus and its trial for prevention in Nile tilapia Oreochromis niloticus. Fatma  M. M. Korni. Global Journal of Fisheries and Aquaculture Res., (2015).2, (3): pp. 47 -63.

 

-Effect of Ginger and its nanoparticles on growth performance, cognition capability, immunity and prevention of Motile Aeromonas Septicemia in Cyprinus carpio fingerlings. Fatma M.M. Korni and Fatma Khalil. Aquaculture nutrition, 2017. pp.1-8. DOI: 10.1111/anu.12526.

 

-Evaluation of Moringa oleifera leaves and their aqueous extract in improving growth, immunity and mitigating effect of stress on common carp (Cyprinus carpio) fingerlings. Fatma Khalil and Fatma M.M. Korni. Turkish journal of aquatic sciences, (2017).  32(3): 170-177.

 

-An outbreak of Motile Aeromonas Septicemia in cultured Nile tilapia, Oreochromis niloticus with reference to hematological, biochemical and histopathological alterations. Fatma M. M. Korni, EL-Shaymaa EL-Nahass  and Walaa M. S. Ahmed. J. Fish Pathol., 2017.  30(1): 11-24.

 

-Safety and effectiveness of trichlorfon in prevention of lernaeosis and its comparison with plant extracts in lernaeosis control. Manal A. A. Essa & Fatma M. M. korni. Aquatic sciences and engineering, 2018; 33(2): 32-38.

 

- The impact of leechiosis on semi-artificial spawning performance and hematological parameters of silver carp (Hypophthalmichthys molitrix) brooders and common carp (Cyprinus carpio) fingerlings  with a reference to its stress response and treatment.  Manal A. A. Essa, Fatma M. M. korni & Walaa M. S. Ahmed. Accepted for publication in Aquatic sciences and engineering, 2018; 33(2): 53-60.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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5-????? ????????? ????????? ?????? ??????  13/3/2012 ??? 15/3/2012.

6-??????? ???????? ?? ?????? ?? 21/2/2012 ??? 23/2/2012.

7- ?????? ?????? ?? ??????? ?????????   3/4/2012 ??? 5/4/2012

8-???? ????? ?????? ?? ????? ???????23/4/2012 ??? 26/4/2012

9- ??????? ??????? ?????????? ?? ??????? ???????? ?? ?????? ??2/5/2012 ??? 3/5/2012

10- ????? ?????? ?????? ???????  8/5/2012 ??? 10/5/2012

1 1- ?????? ????????? ??? ??? ??????? ?????? ?????? ??? ????. 13-3-2016

12- ?????? ?????? ?? ??????? ????????? ?? ?????? ?? 16 ??? 17/ 7 /2017

13- ????? ????? ??????????? ?? ?????? ?? 18 ??? 19/ 7 /2017

14- ?????? ????? ?????? ?? ?????? ?? 18 ??? 19/ 7 /2017

15- ????? ????????? ????????? ?????? ?????? ??????  24/ 7 /2017

16- ??????????? ?????? ??????? ?? ?????? ??  25 ??? 26/ 7 /2017

17- ??????? ??????? ?????????? ?? ??????? ?? ?????? ??  25 ??? 26/ 7 /2017

 

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1-????? ????????? ?? ???? ????????????? ?? ?????? ??17  /5/2009 ??? 20/5/2009

Aquaculture, principles and practices. 26-30 August 2007.

Morphological image analysis, semiquantitative measures for biomarkers, histochemistry, microdissection, PCR, tissue microarray and comet assay. 17-20 may 2009.

4- Nanobiotechnology training course. 3-5 march 2015.

 

 

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1- ????????? ?????????: ????? ????? ?????? ????? ?????? ?? ???? ???????. 16-9-2014

2- ?????? ????????? ??? ??? ??????? ??????. 13-3-2016

3- -??? ??? ????? ?????? ?????? ??????? ?????? ????? ?????? 2015-2016 ?? ????? ??????? ??????? 2012-2017.?? ???????? ?????? 23-6-2016 .

4- ????????? ?????????: ???? ???????? ???????? ?????????. 28-8-2016

5- ??????? ?????? ???? ??????? ??????? ?? ???? ???? ???????. 4-9-2016

6- ?????? ????? ?????? ??????. 18-9-2016

7- ???? ????? ???????? ??????? ???????? ?? ????? ??????. 23-10-2016

8- ?????? ????? ?? ????? ?????? ?????? ???????? ???????. 25-10-2016

9- ????? ????? ???? ??? ???? ??? ??????? ??????. 26-3-2017

10-  ??????? ??????? ??????? ??? ???????? ???????. 14-16 ???? 2017

11- nano-biosensors: Overview, application and prospects. 9-5-2017

12-The Chevening information session. 25-september-2016

 

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-Control of Lernaeosis in Hatchery Reared Cyprinus carpio Fingerlings by Colophony and its Steamed Oil. M.A.A. Abd El-Galil, Manal A.A. Essa, Fatma M.M. Korni. Global Aquaculture & Fisheries Research Conference & Exhibition. Cairo, Egypt, October 24-26 (2009).

 

 -Diagnosis and safe prevention of Edwardsiellosis in Oreochromis niloticus. Manal A.A. Essa, M.M.A. Hussein, M.A.A. Abd El-Galil, and Fatma M.M. Korni. Proceedings of the 5th Global Fisheries and Aquaculture Research Conference. Cairo, Egypt, October 1-3 (2012).

 

-Lernaeosis affecting hatchery reared common carp (Cyprinus carpio) fries and a novel approach for its treatment. Fatma  M. M. Fish Department, Faculty of Veterinary Medecine, Beni-Suef  Univ. Egypt. Global Journal of Fisheries and Aquaculture Res., Vol.1, (2): pp. 173 -189.

-Experimental infection of atypical Aeromonas salmonicida in Nile tilapia Oreochromis niloticus and its treatment with carvacrol and cymene mixture. Fatma Mostafa Mohamed Korni. J. Fish Pathol., (2015).28 (3) : 145-155.

 

- Diagnosis of motile aeromonas septicemia in Catfish Clarias gariepinus and its trial for prevention in Nile tilapia Oreochromis niloticus. Fatma  M. M. Korni. Global Journal of Fisheries and Aquaculture Res., (2015).2, (3): pp. 47 -63.

 

-Effect of Ginger and its nanoparticles on growth performance, cognition capability, immunity and prevention of Motile Aeromonas Septicemia in Cyprinus carpio fingerlings. Fatma M.M. Korni and Fatma Khalil. Aquaculture nutrition, (2017). pp.1-8. DOI: 10.1111/anu.12526.

 

-Evaluation of Moringa oleifera leaves and their aqueous extract in improving growth, immunity and mitigating effect of stress on common carp (Cyprinus carpio) fingerlings. Fatma M.M. Korni and Fatma Khalil. Turkish journal of aquatic sciences, (2017).  32(3): 170-177.

 

-An outbreak of Motile Aeromonas Septicemia in cultured Nile tilapia, Oreochromis niloticus with reference to hematological, biochemical and histopathological alterations. Fatma M. M. Korni, EL-Shaymaa EL-Nahass  and Walaa M. S. Ahmed. J. Fish Pathol., 2017.  30(1) : 11-24.

-Safety and effectiveness of trichlorfon in prevention of lernaeosis and its comparison with plant extracts in lernaeosis control. Manal A. A. Essa & Fatma M. M. korni. Aquatic sciences and engineering, 2018; 33(2): 32-38.

 

- The impact of leechiosis on semi-artificial spawning performance and hematological parameters of silver carp (Hypophthalmichthys molitrix) brooders and common carp (Cyprinus carpio) fingerlings  with a reference to its stress response and treatment.  Manal A. A. Essa, Fatma M. M. korni & Walaa M. S. Ahmed. Accepted for publication in Aquatic sciences and engineering,

 

Master Title

LERNAEOSIS & ITS CONTROL IN HATCHERY REARED CYPRINIDS

Master Abstract

SUMMARY A total number of 10987 cyprinid fish from Abo- saleh fish hatchery visually examined in situ for parasitic copepod. The specimens represented 99 brooders and 3056 fingerlings of C. carpio, 92 brooders and 4246 fingerlings of C. idella and 77 brooders and 3417 fingerlings of H. Molitrix. Random sampling was made monthly from brood and fingerlings stocks of Abo- saleh fish hatchery from the first week of April 2007 till the end week of October 2007. The results showed that the prevalence among brooders and fingerlings stocks of examined cyprinids varied according to the species and fish size, the prevalence among brood stocks of C. carpio, C. idella and H. molitrix was 16.2 %, 14.1 % and 11.7 % respectively and it was 26.5 %, 32 % and 33.1 % among C. Carpio, C. idella and H. molitrix fingerlings respectively. The highest lernaea infestation rates in C. carpio and H. molitrix brooders were detected in June while in their fingerlings were detected in October, in C. idella brooders and fingerlings it was detected in May. The infested fingerlings showed the attachment of worm-like grey to greenish colored copepods with or without paired appendages. L. cyprinacea was distributed along the both sides of the body and peduncle region especially at the base of caudal fin in C. carpio, the parasite attached mostly to the base of the dorsal fin in C. idella and along both sides of the body and the buccal region in H. molitrix. The sites of attachment were characterized by macroscopic reddening and swollen margins (hemorrhagic nodules or tumor-like masses) and these lesions were very clear and healed slowly in the infested C. carpio fingerlings. On the other hands, the sites of lernaea attachment in C. idella and H. molitrix fingerlings were associated with reddening without swollen margins and some times no visible inflammatory signs were detected at these sites especially in C. idella . In additional several lernaea free lesions that were similar to copepod attachment sites were detected. The postmortem examination revealed embedding of the anchors and part of the trunk in the skin and underlying muscles and no changes could be detected by the naked eye in the internal organs of the infested fish. The lernaea infestation significantly decreased the body weight of diseased C. carpio fingerlings where the infested fish were 4.3% up to 71.7% lighter than lernaea free fingerlings and the decrease in the fish s body weight was directly correlated with the intensity of lernaea infestation The histopathological study of skin of C. carpio showed that the epidermis, dermis and hypodermis were infiltrated with massive number of inflammatory cells associated with edema and congested blood vessels, while the C. idella tissue reactions against lernaea infestation were reported as infiltration of massive number of the inflammatory cells in the dermis and hypodermis, acidophilic granular cells in the skeletal muscles as well as subcutaneous tissue and formation of fibrous connective tissue capsule around the embedded part of the parasite. Dipterex did not completely eliminate lernaea infestation from the infested C. carpio fingerlings and the best result obtained was 76.6% reduction in the infestation rate after the 1st treatment, the lowest lernaea infestation rates among the treated C. carpio fingerlings were 10.6% post the 1st treatment and 19.4% post the 2nd treatment The use of colophony either steamed oil or crude resin as drug for control of Lernaeosis in C. carpio fingerlings gave promising results where 5ppm steamed oil of colophony (24 and 48hrs. water bath) was more effective than the crude resin, since the infested fish became lernaea free at 96hrs post bath treatment (24 and 48hrs) comparing with 15.3% of lernaea parasite dropped from the control fish at the same time, while in case of crude colophony the infested fish became lernaea free at 120hrs post 24 and 48hrs direct bath comparing with 14.4% of the parasite dropped from the control fish at the same time. Other advantages of steamed oil included that it was safe than the crude colophony as 100ppm of steamed oil did not cause death for any fish in 24 hrs LC50, while 60ppm crude resin of colophony killed all tested fish and the median lethal concentration (LC50) of crude resin colophony was 46.7ppm for 24 hrs.

PHD Title

Edwardsiellosis in Some Freshwater Fishes

PHD Abstract

Summary A total number of 240 cultured and wild O. niloticus ((75± 5 gms) and 200 wild C. gariepinus (100 ± 10gms) were collected alive from Abo-Saleh fish hatchery (cultured) and khor Abo-Sleem (wild) and fish markets of Beni-suef governorate during spring and summer seasons for determination of edwardsiellosis prevalence. Edwardsiellosis prevalence in spring season was 13.33, 3.33 and 3% among cultured and wild O. niloticus and wild C. gariepinus respectively. On the other hand, in summer season, edwardsiellosis prevalence was 6.7, 0 and 0% among cultured and wild O. niloticus and wild C. gariepinus respectively. At the same time of O. niloticus collections, water samples were collected and examined for studying the effect of water quality on edwardsiellosis prevalence among cultured and wild O. niloticus. In spring season, there was fluctuation in water temperatures (20-30 ºC) and they were some what stable (28.3-29 ºC) in summer season in cultured habitat. DO. levels were high either in spring (8.2-9.4 mg/l) or in summer (6.56-7.4 mg/l). In addition, there was excessive unionized ammonia (NH3) either in spring season (0.3-0.4 mg/l) or in summer (0.5-0.7 mg/l). Highest prevalence of edwardsiellosis (30%) was detected in mid spring, while in the beginning and at the end of spring season and at the beginning and mid of summer season edwardsielosis prevalence was 10%. Similarly, in spring season, there was fluctuation in water temperatures (21-29 ºC) and they were some what stable in summer season (29-30.3 ºC) in wild habitat. DO. levels were low (3.13-4.5 mg/l) in spring season than that of summer season (5.0-6.04 mg/l). In addition, there was excessive unionized ammonia (NH3) either in spring season (0.2-0.3 mg/l) or in summer season (0.3-0.4 mg/l). At the beginning of the spring season edwardsielosis prevalence was 10%. Oppositely, there was no edwardsiellosis among wild O. niloticus at the mid and end of the spring season and all over the period of summer season. Edwardsiellosis naturally infected O. niloticus showed variety of clinical signs including scale-loss, skin darkening, congestion and haemorrhages allover the body. Also, fin rot, exophthalmia, opacity or hemorrhages of the eyes and protruded congested vent were seen. Moreover, abdominal distention, pale or congested liver, distended gall bladder and congested and enlarged spleen were detected. On the other hand, edwardsiellosis naturally infected C. gariepinus showed ulceration of the head, peduncle region and dorsal musculature. In addition, swellings in the dorsal musculature when punctured emit a foul odor, disintegration of dorsal fin and fin rot were seen. The post-mortem lesions were congestion and enlargement of kidney and pale liver and the both organs were soft. Isolation of edwardsiellosis causative agent (E. tarda), from diseased O. niloticus and C. gariepinus was done using SS agar medium. Identification of the pure isolates of E. tarda was carried out on the basis of conventional biochemical tests and confirmed by API 20E biochemical system and PCR technique. The morphological characters of E. tarda colonies on SS agar their variation in size and characterization by black centers to predominantly black colonies. In addition, the results of manual biochemical tests were analogues to that of API 20E biochemical system. The isolates of E. tarda in this study exhibited variation only in citrate utilization test, where, 2 only out of 17 isolates were failed in citrate utilization. By the PCR technique, type 1 fimbrial gene was detected in citrate negative and citrate positive E. tarda isolates which is specific for identification and pathogenicity of E. tarda isolates. The pathogenicity test was carried out on citrate negative & citrate positive E. tarda isolates in O. niloticus by I/P injection of 0.3ml of 3 x 108 CFU/ml (McFarland 1 standard turbidity) and 1.5× 108 CFU/ml (McFarland 0.5 standard turbidity). The results showed that citrate negative isolate was more pathogenic than citrate positive isolate especially at lower concentration (1.5× 108 CFU/ml) as mortality rates were 80 % matching with 20% mortality in the citrate positive isolate. The experimentally infected O. niloticus manifested similar clinical signs of the naturally edwardsiellosis infected fish. The LD50 of citrate negative E. tarda isolate for O. niloticus was 1.5 × 106 CFU/ml. In this study, trials for protection of O. niloticus against edwardsiellosis were conducted by using probiotic, E. faecium in feed. The mortalities were 40% and RLP% was 43% in fish fed E. faecium supplemented diet for one week. Oppositely, no mortalities and 100% RLP% were detected in fish fed E. faecium supplemented diet for two weeks, fish fed E. faecium supplemented diet for two weeks and challenged one week after feeding stop and fish fed E. faecium supplemented diet for two weeks and challenged two weeks after feeding stop. On the other hand, the control group showed 70% mortalities and zero% RLP%. Differential leucocytic counts were characterized by predominance of lymphocytes & monocytes in the blood of O. niloticus fed E. faecium supplemented diet for two weeks, fish fed E. faecium supplemented diet for two weeks and challenged one week after feeding stop and fish fed E. faecium supplemented diet for two weeks and challenged two weeks after feeding stop. Contrary, number of lymphocytes & monocytes decreased in fish fed E. faecium supplemented diet for one week. Granulated leucocytic neutrophiles significantly decreased in the blood of fish fed E. faecium supplemented diet for two weeks and challenged one week after feeding stop and fish fed E. faecium supplemented diet for two weeks and challenged two weeks after feeding stop. Eosinophiles were found only in the blood of fish fed E. faecium supplemented diet for two weeks & fish fed E. faecium supplemented diet for two weeks and challenged two weeks after feeding stop. On the other hand, the basophiles did not found in all groups including the control. Regarding, total protein, albumin, globulin and A/G ratio, there was a significant increase in the total protein and globulin in the fish fed E. faecium supplemented diet for two weeks, fish fed E. faecium supplemented diet for two weeks and challenged one week after feeding stop and fish fed E. faecium supplemented diet for two weeks and challenged two weeks after feeding stop. On the other hand, there was a significant increase in albumin in the fish fed E. faecium supplemented diet for one week, fish fed E. faecium supplemented diet for two weeks, fish fed E. faecium supplemented diet for two weeks and challenged one week after feeding stop and fish fed E. faecium supplemented diet for two weeks and challenged two weeks after feeding stop. A/G ratio in all fish groups did not show significant difference in comparison with control group. Concerning effect of probiotic E. faecium on the level of lysozyme and complement 3, it was found that the level of lysozymes of fish fed E. faecium supplemented diet for one week and fish fed E. faecium supplemented diet for two weeks and challenged two weeks after feeding stop were increased insignificant compared to control group. Lysozymes levels in fish fed E. faecium supplemented diet for two weeks & fish fed E. faecium supplemented diet for two weeks and challenged one week after feeding stop showed significant increase than that of control group. On the other hand, the complement 3 of all experimental groups was insignificantly differed than that of control. The total viable intestinal bacterial counts of O. niloticus fed E. faecium supplemented diet for one week, fish fed E. faecium supplemented diet for two weeks, fish fed E. faecium supplemented diet for two weeks and challenged one week after feeding stop and fish fed E. faecium supplemented diet for two weeks and challenged two weeks after feeding stop were 7×108, 2.2×108, 3×108 & 4×108 CFU g-1 respectively in comparison with 6.1 ± 0.1×109 CFU g-1 in the control group. Another trial for prevention and control of edwardsiellosis in O. niloticus by using plant extracts carvacrol & its biological precursor cymene was done. The synergistic effect of the plant extracts carvacrol and its biological precursor cymene at concentration of 100 ppm and 200 ppm could be able to prevent and control edwardsiellosis in O. niloticus, as there was no mortality in compared to 50% mortality of control group. In addition, fish fed plant extracts supplemented diet appeared silvery bright, active, alert and had good appetite. On the contrary, fish fed non supplemented diet exhibited typical edwardsiellosis clinical signs.

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