Basic Informations
C.V
amr gamal fouad
Master Title
FORMULATION AND EVALUATION OF NANOCARRIER LOADED ANTICANCER DRUG"
Master Abstract
Tamoxifen citrate (TMC) stops and prevents cell multiplication and
development of breast cancer. Novel triaryl (Z)-olefin (TZO) as Tamoxifen
analog was synthesized due to development of resistance, limited activity and
toxicity of TMC. TZO is more effective than TMC by two folds. The aim of
this thesis was to describe an in-situ pH responsive niosomes as a carrier for
localized and sustained delivery of TZO. TZO and TMC loaded niosomes were
developed by Design-Expert software based on User Defined Response
Surface design using different non-ionic surfactants (NIS) [Sorbitan
monostearate (Span 60), Sorbitan monooleate (Span 80) and Polysorbate 80
(Tween 20)] and cholesterol in different molar ratios (1:1, 1:2 and 1:3). The
prepared formulae were characterized for entrapment efficiency (EE), in-vitro
release studies for 8 hours (h) and the vesicle size. TZO and TMC loaded
niosomes formulae prepared using equi-molar ratio of Span 60 and cholesterol
were selected as optimum formulae where they exhibited a distinct nanospherical
shape with EE% up to 88.90% and 91.18% respectively and slow
drug release pattern. Then, the optimized formulae were incorporated into
chitosan and Glyceryl monooleate (C/GMO) solutions as a localized in-situ pH
sensitive hydrogel delivery system. User Defined Response Surface design was
used to determine the effect of C/GMO on viscosity and in vitro release studies
for 8 h. The results displayed that the release rate was significantly antagonistic
with both of C/GMO concentrations while viscosity was significantly
synergistic with both of them. The optimum formulae were selected and
capped with gold as an ideal candidate for computed tomography to evaluate
the efficacy and tissue distribution of TZO compared with TMC using Ehrlich
carcinoma mice model. The optimum formula showed localized TZO in the
tumor and consequently significant anti-tumor efficacy. Based on these results,
the novel in situ pH sensitive TZO loaded niosomes could be a promising
formula for the efficient treatment of breast cancer.
PHD Title
PHD Abstract
PHD