Ahmed Osama El-Gendy

Lecturer of Microbiology and Immunology

Basic Informations

C.V

Curriculum Vitae

Name:     

Ahmed Osama Mostafa El Gendy       

     

Gender

Date of Birth:

Male

January 23, 1984.

Nationality:

Egyptian.

Military service:

Exempted from military services.

Marital status:

Married, with three children.

Position:

Lecturer of Microbiology and Immunology. Faculty of Pharmacy, Beni-Suef University

Address: 

1) El-Gendy Pharmacy, Ahmed Orabi street, Beni-Suef, Egypt.

2) Faculty of Pharmacy, Beni-Suef University, El-Shahid

    Ahmed Hegazi Street, Beni-Suef, 62111, Egypt                                                                                    

Mobile:   

(002) 01223476015

E-mail:

ahmed.elgendy@pharm.bsu.edu.eg

dr.ahmed_micro@yahoo.com

Degrees:

 

1)    B.Sc. Of Pharmaceutical sciences (Excellent with honor degree), Cairo University, Beni-Suef Branch, May, 2005.

2)    Prerequisite graduate courses towards the M.Sc., degree: (with general grade very good), Faculty of Pharmacy, Cairo University, Academic year February 2006, Exams January 2007 and results appeared at April 2007.

3)    M.Sc. of Microbiology and Immunology, Beni-Suef University, March, 2010.

4)    PhD of Microbiology and Immunology, Beni-Suef University, January, 2013.

Noticing that:

1- The research unit at Beni-Suef branch of Cairo University was completed at July 2002.

2- Beni-Suef University, Egypt (was separated from Cairo University according to the Republic role No [192/2005] starting 1 August 2005).

3- All courses and exams (written, oral, and practical) during undergraduate and graduate studies were in English.

Experiences:

1)    Demonstrator of microbiology at the Faculty of Pharmacy, Beni Sueif (Teaching practical course for 2nd, and 3rd year pharmacy students including introduction to practical pharmaceutical microbiology, practical bacteriology, serology and parasitology), 2005-2010.

2)    Visiting researcher at “Laboratory of Microbial Gene Technology and Food Microbiology” at the Department of Chemistry, Biotechnology, and Food Science at the Norwegian University of Life Science during a period from August 2008 - June 2009.

3)    Assistant lecturer in the same faculty, 2010-2013.

4)    Lecturer in the same faculty, 2013 – now.

5)    Lecturer at the faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University from September 2013 – now.

Fields of research and interest:

1)    Antibiotic resistance, virulence factors and CRISPR Cas system associated with Enterococcus faecalis.

2)    Natural products from Actinomycetes isolated from different niches.

3)    Bacteriocins and antimicrobial peptides recovered from lactic acid bacteria (Characterization, Purification and Genotypic analysis)

4)    Mycology, Biofilm & Quorum sensing : Induction of Candida biofilm formation , determination of antifungal susceptibilities of biofilm-grown Candida isolates, measuring extent of penetration of antifungal agents through biofilms, Viable count of biofilm cells exposed to antifungal agents and Scanning electron microscopy (SEM) of Candida albicans biofilm before and after antifungal challenging.

5)    Performing of antimicrobial susceptibility techniques including disk diffusion method and MIC determination for bacterial clinical isolates.

6)    Bacteriology: Isolation and identification of clinical microbial microorganisms (which include E. coli, Klebsiella spp. Enterobacter spp. Staphylococcus spp., Pseudomonas spp.) using variety of media and biochemical tests.

7)    ELISA (Enzyme-linked Immuno-Sorbant Assay) applications.

8)    Molecular techniques (genomics and proteomics) related to bacteriocins encoding genes isolated from lactic acid bacteria.

9)    Bioinformatics: Bioinformatics and its applications in biomedical and pharmaceutical sciences.

Conferences and Most Recent Activities :

1)    Participating with poster in ASM Microbe-2016, which was hold from June 16 – 20, 2016, in Boston, USA.

2)    Participating with poster in FEMS 2015, the 6th Congress of European Microbiologists, which was hold from June 7 – 11, 2015, in Maastricht, the Netherlands.

3)    Participating with oral presentations in the III international conference on Antimicrobial Research – ICAR2014 which was hold from 1-3 October 2014, in Madrid (Spain).

4)    Participating in the International Conference of Nanotechnology, Biotechnology and Spectroscopy "ICNBS 2013" – Tools of Success in the Coming Era, which was hold during 29 Nov. – 01 Dec. 2013, Delta Pyramids Hotel, Egypt.

5)    Participating with oral presentations in the International Scientific Conference of Bacteriocins and Antimicrobial Peptides – BAMP 2013 which was hold during 21st – 23rd May 2013, in the university city of Kosice, Slovakia, in Doubletree Hilton Hotel.

6)    Participating in the second scientific symposium of Faculty of Pharmacy, Beni-Suef University on "Recent Advances In Pharmaceutical Research", Jan. 12th, 2011.

7)    Attendance a training course of bioinformatics and recent applications of gene technology, at faculty of pharmacy, Cairo University, September 2010.

8)    Member of the Egyptian Pharmacists Syndicate.

9)    Member of American society for microbiologists (ASM).

10)           Member of the Cairo University Staff Association.

11)    Member of the Egyptian Pharmaceutical Society.

12)    Director of Innovation, Patency, Research excellence Support Office at Beni-Suef University from the period of Oct. 2014 – until June 2016.

13)    Director of Computer center at Faculty of Pharmacy, Beni-Suef University from the period of 2009 – 2011.

14)    Assistant director of RDI Focal point and international relationship office, Beni-Suef University from the period of 2010 – 2012.

   14) Elected as a Member of the Board Directors of Beni-Suef Pharmacists 

         Syndicate from the period of 2011 – 2014.

Thesis:

Ahmed Osama El-Gendy, (2010). "Studying of Candida albicans Biofilms and Their Susceptibility to Antifungal Agents". Thesis for M. Sc. degree. Faculty of Pharmacy, Beni-Suef University.

 

Ahmed Osama El-Gendy, (2013). "Characterization of Enterococcus faecalis isolated from intensive care units and food samples, and testing their ability for bacteriocins production". Thesis for PhD degree. Faculty of Pharmacy, Beni-Suef University.

Publications:

1) Abdelgawad, M. A., Bakr, R. B., El-Gendy, A. O., Kamel, G. M., Azouz, A. A., & Bukhari, S. N. A. (2017). Discovery of a COX-2 selective inhibitor hit with anti-inflammatory activity and gastric ulcer protective effect. Future Medicinal Chemistry, 9(16), 1899-1912.


2) Fahim, H. A., El Rouby, W. M., El-Gendy, A. O., Khairalla, A. S., Naguib, I. A., & Farghali, A. A. (2017). Enhancement of the productivity of the potent bacteriocin avicin A and improvement of its stability using nanotechnology approaches. Scientific Reports, 7, 10604.


3) Ahmad, M. S., El-Gendy, A. O., Ahmed, R. R., Hassan, H. M., El-Kabbany, H. M., & Merdash, A. G. (2017). Exploring the Antimicrobial and Antitumor Potentials of Streptomyces sp. AGM12-1 Isolated from Egyptian Soil. Frontiers in microbiology, 8.

4) AbdEl-Mogheith, S., El-Gendy, A. O., Sultan, S., & El-Nesr, K. A. Exploring the Antimicrobial and Hepatoprotective Effects of Kefir; A Probiotic Fermented Milk. Journal of pure and applied microbiology 11 (2), 759-772.

5) Aly, A. M., Adel, A., El-Gendy, A. O., Essam, T. M., & Aziz, R. K. (2016). Gut microbiome alterations in patients with stage 4 hepatitis C. Gut pathogens, 8(1), 42.

6) El-Hawary, S., Mohammed, R., AbouZid, S., Ali, Z. Y., El-Gendy, A. O., & Elwekeel, A. (2016). In-vitro cyclooxygenase Inhibitory, antioxidant and antimicrobial activities of phytochemicals isolated from Crassula arborescens (Mill.) Willd. International Journal of Applied Research in Natural Products, 9(4), 8-14.

7) Hassan M H A, Mohammed R, Hetta M H, Abdelaziz T A, El-Gendy A O, Sleim M A (2016). Biological and Chemical Investigation of the Soft Coral Lobophytum pauciflorum Collected from the Egyptian Red Sea. International Journal of Pharmacognosy and Phytochemical Research 8 (6), 906-911.

8) Fahim, H. A., Khairalla, A. S., & El-Gendy, A. O. (2016). Nanotechnology: a valuable strategy to improve bacteriocin formulations. Frontiers in microbiology, 7 .‏                                                                                                                                         

9) El-Gendy AO, Gaber A., Rashad R,, Hassan H., Sayed M. (2016). Purification and characterization of potent antimicrobial agent with antitumor activity recovered from Actinomycetes in Beni-Suef, Egypt. ASM Microbe-2016, June 16 – 20, 2016, in Boston, USA.

10) Mahmoud A, Adel A, El-Gendy AO, Essam T, Aziz RK (2015). A pilot exploration of the gut microbiome of Egyptian HCV patients. The 13th Annual Rocky Mountain Bioinformatics Conference (Rocky 2015; http://www.iscb.org/rocky2015) December 10-12, 2015, in Snowmass, Colorado, USA. “Rocky” is an official conference of the International Society for Computational Biology (ISCB), and serves the worldwide bioinformatics research community.

11) Hassana GS, Awdallah FM, El-Saadi MT, El-Gendy AO, Hemeda LR (2015). Synthesis of some benzothiazole derivatives evaluated as antimicrobials and antibiofilms. Journal of Chemical and Pharmaceutical Research, 7 (7): 1125-1143.

12) Mebad ZM, Abdelghani SMM, El-Gendy AO and Amin MA (2015). Comparative Studies on the Antimycotic Activities of Commercially Available Vaginal Douches in the Egyptian Market. New Egyptian Journal of Microbiology, September 2015, ISSN; 1687-1219.

13) Lamie PF, Philoppes JN, El-Gendy AO, Rarova L, Gruz J (2015). Design, Synthesis and Evaluation of Novel Phthalimide Derivatives as in Vitro Anti-Microbial, Anti-Oxidant and Anti-Inflammatory Agents. Molecules 2015, 20(9), 16620-16642; doi:10.3390/molecules200916620

14) Mebad ZM, Abdelghani SMM, El-Gendy AO and Amin MA (2015). Commercial vaginal douches enhanced various virulence factors of Candida albicans isolated from clinical specimens of Egyptian women.  ICAAC/ICC2015 conference, San Diego, California. September 17-21.

15) El-Gendy AO, Hendawy HAM, Ibrahim AM (2015). Fabrication of DNA electrochemical biosensor based on carbon nanotubes for detection of fecal contaminated water with Enterococcus faecalis. FEMS 2015, the 6th Congress of European Microbiologists, which was hold from June 7 – 11, 2015, in Maastricht, the Netherlands.

16) Dundar H, Brede DA, La Rosa SL, El-Gendy AO, Diep DB, Nes IF (2015). Fsr quorum sensing system and cognate Gelatinase orchestrate the expression and processing of pro-protein EF1097 into mature antimicrobial peptide enterocin O16. J Bacteriol. 2015 Jul;197(13):2112-21. doi: 10.1128/JB.02513-14. Epub 2015 Mar 2.

17) El-Gendy AO (2014). Different growth kinetics and their impacts on production of enterocin OS13 following by applying different purification strategies for recovering of high yield bacteriocin. International conference on Antimicrobial Research – ICAR2014 which was hold from 1-3 October 2014, in Madrid (Spain).

18) Tarek N, Hassan HM, AbdelGhani SMM, Radwan IA, Hammouda O, El-Gendy AO (2014). Comparative chemical and antimicrobial study of nine essential oils obtained from medicinal plants growing in Egypt. Beni-Suef University Journal of Basic and Applied Sciences, Volume 3, Issue 2, June 2014, Pages 149–156.

19) El-Gendy AO, Brede DA, Essam TM, Amin MA, Ahmed SH, Holo H, Nes IF (2013). Purification and characterization of Enterocin OS13 α & β; novel bacteriocins from a food isolate Enterococcus faecalis OS13 with potent activity towards antibiotic resistant nosocomial enterococci. International Scientific Conference of Bacteriocins and Antimicrobial Peptides – BAMP 2013 which was held during 21st – 23rd May 2013, in the university city of Kosice, Slovakia.

20) Ahmed, SH, Amin, MA, Saafan, AE, El-Gendy, AO, ul Islam, M, (2013). Measuring susceptibility of Candida albicans biofilms towards antifungal agents. Der Pharmacia Lettre, 5 (1):376-383 .

21) El-Gendy AO, Essam TM, Amin MA, Ahmed SH, Nes IF, (2012). Clinical Screening for Bacteriocinogenic Enterococcus faecalis Isolated from Intensive Care Unit Inpatient in Egypt. J Microb Biochem Technol., 4: 161-167. doi:10.4172/1948- 5948.1000089.

22) Ahmed SH, Amin MA, Saafan AE, El-Gendy AO, (2011). Antifungal Susceptibilities and Penetrations of Planktonic and Sessile Candida albicans Isolated from Urinary Tract Infections and Vaginitis from Beni-Suef City, Egypt. Egy J Medical Microbiol., 20(4): 107-121

 

Projects:

  1. Principal Investigator in the collective project funded by the Beni-Suef University, entitled "Nanocomposites, a novel approach to optimize, purify and enhance the antibacterial activity of a potent bacteriocin (Enterocin OS13)", 2014. (65,000 EGP., Beni-Suef University).
  2. Researcher in the project funded by the Beni-Suef University, entitled "Prediction of the response to interferon and rebavirin treatment in Egyptian chronic hepatitis C virus infection by investigation of interleukins 10, 12 and 18 and il-28b single nucleotide polymorphism", 2014. (40,000  EGP., Beni-Suef University).
  3. Principal Investigator in the project funded by the Beni-Suef University, entitled "High throughput investigation for novel antimicrobial compounds recovered from different wild types microbiota designed for pharmaceutical applications", 2015. (50,000 EGP., Beni-Suef University).
  4. Researcher in the collective project funded by the Beni-Suef University, entitled "Target nano particles for treatment of chronic bacterial proestatitis", 2015. (75,000 EGP., Beni-Suef University).
  5. Researcher in the project funded by the Beni-Suef University, entitled "Bioactive metabolites isolated from aquatic microorganisms of Nile River", 2015. (50,000 EGP., Beni-Suef University).

Memberships:

1)    Member of American society for microbiologists (ASM).

2)    Member of the Egyptian Pharmacists Syndicate.

Patents:

Layered double hydroxide nanoparticles are a novel approach to increase stability of Avaicin A. Submitted in Egypt patent office 2017/587.

Main managerial responsibilities:

1)    Assistant director of RDI Focal point and international relationship office, Beni-Suef University from the period of 2010 – 2012.

2)    Director of Computer center at Faculty of Pharmacy, Beni-Suef University from the period of 2009 – 2011.

3)    Elected as a Member of the Board Directors of Beni-Suef  

Pharmacists Syndicate from the period of 2011 – 2014.

4)    Director of Innovation, Patency, Research excellence Support Office at Beni-Suef University from the period of Oct. 2014 – until June 2016.

5)    Co-director for clinical pharmacy program at Faculty of Pharmacy, Beni-Suef University from the period June 2016- until June 2017.

 

Master Title

Studying of Candida albicans Biofilms and Their Susceptibility to Antifungal Agents

Master Abstract

Master Thesis Abstract By: Ahmed Osama El-Gendy “Studying of Candida albicans Biofilms and Their Susceptibility to Antifungal Agents” Biofilms represent the most prevalent type of microbial growth in nature and are crucial to the development of clinical infections. They can serve as a cause for disease and are often associated with high-level antimicrobial resistance of the associated organisms. The objectives of this work were to study the effect of different antifungal drugs on the preformed C. albicans biofilm and to study the antifungal penetration pattern as a mechanism of biofilm resistance. A total of 152 isolates of Candida isolates were collected from different clinical specimens from Egyptian hospitals. They were identified as C. albicans (64); C. tropicalis (42); C. krusei (35); and C. glabrata (11). The antifungal sensitivity pattern and the antifungal`s minimum inhibitory concentrations (MICs) of the tested planktonic C. albicans was determined using the agar dilution method on RPMI-1640 medium. The obtained data showed that nystatin showed activity against all tested isolates followed by amphotericin B and clotrimazole which showed resistance by only 3 isolates. Determinations of antifungal susceptibilities and degree of adherence of biofilm-grown Candida isolates were done in 96-well microtiter plates using RPMI medium and using the semi-quantitative XTT-reduction assay. For many years, the XTT assay has been the mainstay for the estimation of biomass in yeast biofilms. The tetrazolium salt XTT is intracellularly reduced to a water soluble formazan, which is colorimetrically determined in the cell supernatant. The obtained results showed that biofilm originated from vaginal isolates were more complicated with high degree of adherence than those from catheters and urinary tract infections. C. albicans cells in biofilm conditions display dramatically increased resistance to antifungal agents compared to that of cells in planktonic conditions. This work moved then to study the different stages and kinetic of biofilm formation on the wells of microtiter plates over 48 hrs. The results showed that the biofilms were highly metabolically active after the first 12 hours but the complexity increased after (24 to 48 hrs). A marked decrease in the complexity and the cellular density of the formed biofilm when exposing C. albicans to planktonic subinhibitory levels of antifungal agents and these results may point to approaches for preventive or prophylactic treatment. A severe drop in the finally formed biofilm was obtained when adding nystatin at concentration equal to biofilm sub SMIC50 after 3 and 6 hrs. While, there was a paradoxical rise in metabolic activity of mature biofilms when adding nystatin after 12 or 24 hrs. An investigation and evaluation of the penetration of antifungal agents through 48-h biofilms (as a possible mechanism that may protect microorganisms in biofilms from antibiotics) using polycarbonate membrane filter were done. The results demonstrated that azole antifungal agents permeated all Candida biofilms more rapidly than terbinafine and polyene antifungal agents. Viability of biofilm cells after exposure to antifungal agents for 24 h reveals the failure of penetrated drug to produce complete killing of biofilm cells. Although, amphotericin B was the least penetrant through biofilms but viable count observations revealed that it caused the most damage to the biofilm cells in comparison to fluconazole. These results indicate that poor drug penetration is not a major resistance mechanism for Candida biofilms. Scanning electron microscope (SEM) revealed that the fully mature biofilms is produced after incubation for up to 48 hours and it is consisted of a dense network of yeast cells. There was a wrinkled, ruptured, and ballooning effect of the drug on yeast cells after applying nystatin in its inhibitory concentration on 24-h biofilms.

PHD Title

Characterization of Enterococcus faecalis isolated from intensive care units and food samples, and testing their ability for bacteriocins production

PHD Abstract

PhD Thesis Abstract By: Ahmed Osama El-Gendy “Characterization of Enterococcus faecalis isolated from intensive care units and food samples, and testing their ability for bacteriocins production” Enterococcus faecalis belongs to lactic acid bacteria which are commonly known to produce antimicrobial peptides called bacteriocins. In this study, 38 E. faecalis isolates were isolated from food samples (n=11) and clinical samples (n=27). These Enterococcus isolates were identified to the genus level depending on microscopical examination, culture characters of colonies on bile esculin agar, testing for lactic acid production and PYR test. Identification to the species level was based on sequencing of 16s rRNA gene. Phenotypic characterization of some virulence factors belonging to Enterococcus isolates revealed that 21 E. faecalis isolates (55.3%) were able to hydrolyse gelatin as a result of proteolytic gelatinase activity. A number of 12 E. faecalis isolates (31.5%) were able to make complete blood hemolysis (ß hemolysis) as a result of cytolysin activity. A number of 22 E. faecalis isolates (57.9%) were able to hydrolyse bile salts as a result of bile salt hydrolase activity. Three strains isolated from food samples and nine strains isolated from clinical samples showed an ability to produce bacteriocins. Continuing phenotypic characterization for bacteriocinogenic isolates was done by detection of fermentation profile using (API 50 CH) system and antibiotic resistant pattern. All bacteriocinogenic E. faecalis isolates were sensitive to Ampicillin and Vancomycin while all of them were resistant to Polymyxin B, Clindamycin and Fusidic acid. Genotypic characterization for bacteriocinogenic isolates was done by investigating the epidemiology and population structure by a mean of multi locus sequence typing (MLST) of 7 housekeeping genes and that resulted in presence of these isolates in between sequence types 116, 141 and 6. The presence of 17 common bacteriocins encoding genes were analyzed and revealed absence of any structural genes corresponding to bacteriocins production except Enterolysin A and Cytolysin genes which were only detected in one bacteriocinogenic strain OS6. The E. faecalis OS13 isolate was shown to produce a large amount of narrow spectrum and highly potent bacteriocin named enterocin OS13. Enterocin OS13 was purified and is comprised of two novel bacteriocin peptides that inhibited the growth of antibiotic resistant nosocomial E. faecalis and E. faecium isotates. The two peptides designated enterocin OS13a and enterocin OS13ß were purified to homogeneity from the culture supernatants by ammonium sulphate precipitation, cation-exchange chromatography and reverse phase chromatography. The molecular weight of enterocin OS13a and enterocin OS13ß was determined to be 8079 Da and 7859 Da, respectively. Both bacteriocins are heat labile. Enterocin OS13a is sensitive to proteinase K enzyme while enterocin OS13ß is resistant. E. faecalis OS13 belongs to the sequence type 116 (ST116) and is being ?-haemolytic, bile salt hydrolase negative, and gelatinase positive phenotypes. Antibiotic sensitivity test showed that the OS13 isolate is sensitive to ampicillin, penicillin, vancomycin, erythromycin, kanamycin and gentamicin. In conclusion, nosocomial infections caused by multiresistant Enterococci are an ever increasing problem. Antibiotic resistant clones colonize the hospitals and acquire and transmit antibiotic resistance by horizontal gene transfer. Enterococcal infections refractive to vancomycin, methicillin and daptomycin occur. Thus novel therapeutic agents for treatment of such infections are needed. Bacteriocins producing strains or just their bacteriocins have been predicted to be useful in the control of gastrointestinal microflora, and in the protection against pathogens. To the best of our knowledge, this is the first time to report and identify Enterolysin A in between pathogenic E. faecalis isolated from clinical specimen collected from human in Egypt. From our knowledge, this is the first time for E. faecalis with ST116 to be isolated from food sample. Bacteriocin peptides like the enterocinOS13 a & ß represent novel antimicrobial agents perfectly designed to combat multiresistant nosocomial Enterococcal infections, without disturbing commensal microflora. However, several safety criteria should be taken into consideration for bacteriocinogenic E. faecalis before being used as a probiotic culture to improve microbiological quality of fermented foods or medically used to fight antibiotic resistant bacteria.

All rights reserved ©Ahmed Osama El-Gendy