Research Article

Further evaluation of locally prepared live attenuated bovineephemeral fever vaccine in cattle

Ibrahim, M.M., Amany El Zieny, Christine A. Mikhael, Soliman Y.A.
Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt. (CLEVB)

Abstract

Bovine ephemeral fever (BEF) is an acute, arthropod-borne disease of cattle. The disease is characterized by sudden onset of fever, high morbidity and very low mortality. Recovery occurs within three days of the onset of clinical signs. BEF is an important viral disease of cattle in Egypt so the live attenuated BEFV vaccine which is inactivated just before inoculation by reconstitute in PBS containing saponin. is extensive used for the prevention and control of the disease. Different assays were applied in the current study to quality control evaluate of that produce vaccine by detection of viral identity and viability before and after reconstitution by using real time quantitative reserve transcriptase polymerase chain reaction (qRT-PCR) and clinical findings (Body temperature and clinical signs) and potency by measuring the humoral immune response by serum neutralization test (SNT) and ELISA and cellular immune response by interferon gamma (IFN-γ) using ELISA kit and Quantitative real time polymerase chain reaction (qRT-PCR) and also by lymphocyte cell proliferation assay using tetrazolium salt(XTT).

Keywords

Bovine Ephemeral Fever Virus, Vaccines, Evaluation, qRT-PCR, Saponins.